Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Kloramin-T Uygulaması Sonucu Solungaçlarda Meydana Gelen Oksidatif Stresin Beyin Dokusundaki c-Fos ve BDNF Gen Ekspresyonu Üzerine Etkisinin Araştırılması

Year 2019, Volume: 6 Issue: 1, 78 - 83, 21.01.2019

Harun Arslan , Selçuk Özdemir

https://doi.org/10.30910/turkjans.515353

Cited By: 1

Abstract

Kloramin-T, balıklarda
solungaç hastalıklarını önlemek ve/veya bu hastalıklardan korunmak için
kullanılan bir kimyasaldır. Birçok balık türünde solungaçlarda oluşan
bakteriyel hastalıklar kloramin-T uygulanarak kontrol altına alınmaktadır.
Ancak yanlış kloramin-T uygulaması sucul canlılarda oksidatif strese neden
olmaktadır. Bu çalışmada, gökkuşağı alabalıklarına (Oncorhynchus mykiss) uygulanan kloramin-T’nin solungaçlarda
oluşturacağı oksidatif stres düzeyinin araştırılması ve bu stresin beyindeki
nöronal aktiviteyi etkileyip etkilemediğinin araştırılması amaçlanmaktadır. Bu
amaç için gökkuşağı alabalıklarına sub-lethal dozda (2.8 mg/L) 0-1-24-48-72 ve
96 saat kloramin-T uygulaması yapıldı. Daha
sonra antioksidant enzimleri kodlayan genlerin ( süperoksit dismütaz 1 (SOD1), süperoksit dismütaz 2 (SOD2), katalaz (CAT), glutathion peroksidaz 1 (GPX1),
glutathion peroksidaz 4 (GPX4)) mRNA
transkript seviyeleri qRT-PCR ile ölçüldü. Son olarak beyin dokularında nöronal
aktiviteyi ölçmek amacıyla c-Fos ve BDNF genlerine ait ekspresyon seviyeleri
yine qRT-PCR ile belirlendi. Kloramin-T uygulanan balıkların solungaçlarında SOD1, SOD2, CAT ve GPX1 genlerinin ekspresyon seviyelerinin kontrol grubuna göre
önemli derecede arttığı gözlemlendi. Ancak GPX4
geninde önemli bir artış gözlemlenmedi. Aynı balıkların beyin dokuları
incelendiğinde c-fos ve BDNF ekspresyonlarının benzer şekilde
kontrol grubuna göre artış gösterdiği belirlendi. Çalışmadan elde edilen bu
sonuçlar, aşırı ve/veya yanlış kloramin-T uygulamasının gökkuşağı
alabalıklarının solungaç dokularında oksidatif strese neden olduğunu ve oksidatif
strese maruz kalan balıkların beyin dokularında nöronal aktivitenin negatif
yönde etkilendiğini göstermektedir.

Keywords

Kloramin-T, oksidatif stres, gökkuşağı alabalığı, c-Fos, BDNF.

Investigation of the Effect of Oxidative Stress in Gill Tissue on the Brain Tissue c-Fos and BDNF Gene Expression after Chloramine-T Application of Rainbow Trout (Oncorhynchus mykiss)

Abstract

Chloramine-T is a chemical used to prevent and/or protect gill diseases
in fish. In many fish species, bacterial diseases that occur in the gills are
controlled by chloramine-T application. However, the wrong application of
chloramine-T causes oxidative stress in aquatic organisms. In this study, it is
aimed to investigate the oxidative stress level of chloramine-T applied to
rainbow trout (Oncorhynchus mykiss)
in gills and to investigate whether this stress affects neuronal activity in
brain. For this purpose, rainbow trout were used sub-lethal dose (2.8 mg / L)
0-1-24-48-72 and 96 hours of chloramine-T. The levels of mRNA transcripts of
genes encoding antioxidant enzymes (superoxide dismutase 1 (SOD1), superoxide
dismutase 2 (SOD2), catalase (CAT), glutathione peroxidase 1 (GPX1), glutathione
peroxidase 4 (GPX4)) were measured by qRT-PCR. Finally, expression levels of
c-Fos and BDNF genes were determined by qRT-PCR to measure neuronal activity in
brain tissues. It was observed that the expression levels of SOD1, SOD2, CAT
and GPX1 genes increased significantly in the gills of fish treated with
chloramine-T. However, there was no significant increase in GPX4 gene. When the
brain tissues of the same fish were examined, it was determined that c-fos and
BDNF expressions increased in comparison to the control group. The results of
the study show that excessive and/or incorrect chloramine-T application causes
oxidative stress in the gill tissues of rainbow trout and negative effects of
neuronal activity on brain tissues of fish exposed to oxidative stress.

Keywords

Chloramine-T, oxidative stress, rainbow trout, c-Fos, BDNF

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