Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis)

Year 2019, Volume: 6 Issue: 1, 97 - 105, 21.01.2019

Hülya Torun

https://doi.org/10.30910/turkjans.515361

Cited By: 1

Abstract

Plants acclimation to stress is a complex process and this complexity increase with simultaneous
subjection to two or more abiotic stress. Salinity and excess accumulation of nickel are growth and development
restricted abiotic stress agents. The aim of the present study was to determine the antioxidant defense system
responses of Melissa officinalis (lemon balm) to salinity, nickel and their combinations. Growth, leaf osmotic
potential, chlorophyll fluorescence, relative water content (RWC), lipid peroxidation, H2O2 and proline content
and antioxidant enzyme activities were determined under these stress conditions. Growth and leaf osmotic
potential negatively affected due to the combined effect of salt and nickel. All three stress treatments did not
show any significant change on RWC and chlorophyll fluorescence in lemon balm. H2O2 content and lipid
peroxidation of combined stress had greater than salt and nickel alone. Total activities of ascorbate peroxidase
(APX) and glutathione reductase (GR) were increased with salt, nickel and their combinations. Moreover, the
reduction in superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) activities resulted in higher lipid
peroxidation and H2O2 content under the combined stress. To the best of our knowledge, this is the first study
conducted on the activities of antioxidant defense enzymes of lemon balm under salinity and nickel
combinations.

Keywords

Melissa officinalis, Abiotic stress combination, antioxidant enzyme, lemon balm, nickel

Kombine Tuz ve Nikel Stresinin Limon Otu (Melissa officinalis)'nun ROS Üretimi ve Antioksidan Enzim Aktiviteleri Üzerine Etkisi

Abstract

Bitkilerin strese alışması karmaşık bir süreçtir ve bu karmaşıklık iki veya daha fazla abiyotik strese aynı anda maruz kalındığında artar. Tuzluluk ve aşırı nikel birikimi büyüme ve gelişimi sınırlandıran abiyotik ajanlardır. Bu çalışmanın amacı, Melissa officinalis (limon otu) bitkisinde tuzluluk, nikel ve bunların kombinasyonlarına karşı antioksidan savunma sistem cevaplarını belirlemektir. Bu stres koşulları altında, büyüme, yaprak ozmotik potansiyeli, klorofil floresansı, nisbi su içeriği (RWC), lipid peroksidasyonu, H₂O₂ ve prolin içeriği ve antioksidan savunma enzimleri belirlenmiştir. Büyüme ve yaprak osmotik potansiyeli tuz ve nikel kombinasyonları nedeniyle olumsuz etkilenmiştir. Her üç stres uygulaması, limon otunun RWC ve klorofil floresansında önemli değişiklik göstermemiştir. Birleşik stres altındaki H₂O₂ içeriği ve lipid peroksidasyonu, tuz ve nikelin tekli uygulamalarından daha yüksektir. Askorbat peroksidaz (APX) ve glutatyon redüktaz (GR)’ın toplam aktiviteleri tuz, nikel ve kombinasyonları ile artmıştır. Dahası, süperoksit dismutaz (SOD), peroksidaz (POX) ve katalaz (CAT) aktivitelerindeki düşüş, birleşik stres altında yüksek lipid peroksidasyonu ve H₂O₂ içeriğinin artması ile sonuçlanmıştır. Bildiğimiz en iyi şekilde, bu çalışma tuzluluk ve nikel stresleri kombinasyonları altında limon otunun antioksidan savunma enzim aktivitelerinin yürütüldüğü ilk çalışmadır.

Keywords

Melissa officinalis, Abiyotik stres kombinasyonu, antioksidan enzim, limon otu, nikel

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