Farklı Sıcaklık ve pH’ın Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Aksillar Sürgün Rejenerasyonu Üzerine Etkisi

Year 2019, Volume: 6 Issue: 4, 826 - 834, 16.10.2019

Muhammet Dogan

https://doi.org/10.30910/turkjans.633612

Abstract

Bitki doku kültürü çalışmalarında sıcaklık ve pH
önemli faktörlerdir. Bu faktörlerin optimizasyonu çoklu bitki üretimi için
önemlidir. Bu çalışmada, farklı sıcaklık (15-30°C) ve pH (5.5-6.6) koşullarında
Rotala rotundifolia (Buch-Ham. ex
Roxb) Koehne’nın etkili ve hızlı üretimi için bir optimizasyon çalışması
amaçlanmıştır. Sıcaklık çalışmalarında, ilk sürgün rejenerasyonları 25°C’de 12.
günde gözlenmiştir. En yüksek sürgün rejenerasyon frekansları (%100) 20 ve
25°C’de elde edilmiştir. Maksimum eksplant başına sürgün sayısı (23.38 adet) ve
en uzun sürgünler (1.74 cm) 25°C’de elde edilmiştir. Farklı pH uygulamalarında,
en yüksek sürgün rejenerasyon oranları (%100) pH 5.5 ve 6’da tespit edilmiştir.
En fazla eksplant başına sürgün sayısı (20.44 adet) ve en uzun sürgünler (1.71
cm) pH 6’da kaydedilmiştir. Eksplantlar genellikle yüksek pH değerlerinde daha
uzun sürgünler vermiştir. Kültür ortamında üretilen sürgünler köklendirildikten
sonra akvaryum ortamına başarıyla alıştırılmıştır. Sonuç olarak, R. rotundifolia’nın in vitro üretimi için optimum değerler 25°C sıcaklık ve pH 6 olarak
bulunmuştur.

Keywords

Doku kültürü, pH, sıcaklık, sürgün rejenerasyonu

The Effect of Different Temperature and pH on Axillary Shoot Regeneration of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Abstract

Temperature and pH are important factors in plant tissue culture
studies. The optimization of these factors is important for multiple production
of plants. In this study, an optimization study for the efficient and rapid
production of Rotala rotundifolia (Buch-Ham.
ex Roxb) Koehne in different temperature (15-30°C) and pH (5.5-6.6) conditions
was aimed. In temperature studies, the first shoot regeneration was observed at
25°C on the 12th day. The highest shoot regeneration frequencies (100%) were
obtained at 20 and 25°C. The maximum number of shoots per explant (23.38) and
the longest shoots (1.74 cm) were obtained at 25 °C. In different pH
applications, the highest shoot regeneration rate (100%) was determined at pH
5.5 and 6. The maximum number of shoots per explant (20.44) and the longest
shoot (1.71 cm) were recorded at pH 6. The explants usually gave longer shoots
at higher pH values. After the shoots produced in the culture medium were
rooted, they were successfully used in the aquarium environment. As a result,
the optimum values for in vitro
production of R. rotundifolia were
found to be 25°C temperature and pH 6.0.

Keywords

Tissue culture, pH, temperature, shoot regeneration

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