In vitro Suppression Effects of Rhizobacteria against Pseudomonas syringae pv. tomato the Agent of Bacterial Speck Disease of Tomato

Year 2020, Volume: 7 Issue: 1, 283 - 289, 25.01.2020

Gül İmriz

https://doi.org/10.30910/turkjans.680101

Abstract

Bacterial speck caused by Pseudomonas syringae pv. tomato is significant pathogen of tomato causing economical losses in yield and quality. Bactericides for the control of such plant diseases are insufficient in some cases. Plant Promote Growth Promoting Rhizobacteria is recently considered as a potential alternative approach to the control of plant pathogens. Here, a collection of bacteria (thirty-eight in total) obtained from rhizospheric soil of different plants were examined for in vitro antagonistic effects on the Pseudomonas syringae pv. tomato growth on agar plates through dual culture assay. Seven isolates out of thirty-eight exhibited effects with varying ranges of inhibition zones from 0.1 to 0.867 cm on the pathogen isolate obtained from Çumra district of Konya. Based on MALDI Biotyper classification results, all of the rhizobacterial isolates showing in vitro antagonistic actions were identified as Bacillus genera, excluding an isolate that was determined as belonged to Paenibacillus genera.

Keywords

Antagonism, Bacteria, Disease, In vitro

Domates Bakteriyel Benek Hastalığı Etmeni Pseudomonas syringae pv. tomato’nun Üzerinde Rhizobakterilerin In vitro’ da Baskılayıcı Etkileri

Abstract

Pseudomonas syringae pv. tomato’ nun neden olduğu bakteriyel benek domateste verim ve kalitede ekonomik olarak kayıplara neden olan önemli bir patojendir. Bazı durumlarda bu tür bitki hastalıklarının kontrolünde bakterisit uygulamaları yetersiz kalmaktadır. Son zamanlarda bitki patojenlerinin kontrolünde Bitki Büyümesini Destekleyen Rizobakteriler potansiyel bir alternatif yaklaşım olarak görülmektedir. Bu çalışmada, farklı bitkilerin rizosfer toprağından elde edilen bir bakteri koleksiyonunun (otuz sekiz adet), patojen bakterinin besi ortamında büyümesi üzerinde in vitro antagonistik etkileri ikili kültür yöntemi ile belirlenmiştir. Çalışmada kullanılan Pseudomonas syringae pv. tomato izolatı Konya-Çumra’da açık alan üretimi yapılan domates bitkilerinden izole edilmiştir. Otuz sekiz rizobakteriyel izolattan yedi tanesi, besi üzerinde 0.1 ila 0.867 cm arasında değişen inhibisyon zonları oluşturarak Pseudomonas syringae pv. tomato’ ya karşı etkinlik göstermişlerdir. MALDI Biotyper sınıflandırma sonuçlarına göre, Paenibacillus cinsine ait olduğu belirlenen bir izolat dışında in vitro’ da antagonistik etki gösteren rizobakteriyel izolatların tümünün Bacillus cinsine olduğu tespit edilmiştir.

Keywords

Antagonizm, Bakteri, Domates, Hastalık, In vitro

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