Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Year 2020, Volume: 7 Issue: 4, 855 - 866, 20.10.2020

Abdullah Güller Mustafa Usta

https://doi.org/10.30910/turkjans.727892

Cited By: 6

Abstract

Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch’s broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the “Canditatus Phytoplasma solani” (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the “Canditatus Phytoplasma trifolii” (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as ‘Bingöl D11’ and ‘Bingöl D90’ isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong.
This current paper is documented in the first record of “Ca. P. solani” (16SrXII-A) and “Ca. P. trifolii” (16SrVI-A) in naturally diseased tomato in Bingol of Turkey.

Keywords

“Ca. P. solani”,, “Ca. P. trifolii”,, Virtual RFLP,, 16S rRNA gene,, tomato

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Abstract

Bingöl ilinde yetiştiriciliği yapılan domates (Lycopersicum esculentum L.) tarlalarında fitoplazma benzeri belirtiler görülmüştür. Dikkat çeken belirtiler arasında cadı süpürgesi, rozetleme, mor renkli kıvrılmış ve gevrek yapraklar, üst dallarda küçük yapraklılık ve aşırı uzamış kaliks yer almaktadır. Toplanan 8 simptomlu ve simptomsuz bitkinin yapraklarından muhtemel patojeni belirleyebilmek için genomic DNA izolasyonu yapıldı. Universal primer setleri kullanılarak gerçekleştirilen Nested-PCR testinde, 8 örneğin 4’ünde beklenen yaklaşık 1200 bp büyüklüğünde DNA bandları elde edilmiştir. R16F2n/R16R2 primerleri ile band veren örneklerden rastgele ikisi seçilerek uygun bir plasmid vektörde klonlanmıştır. Saflaştırılan rekombinant plasmid DNA’sı çift yönlü olarak dizilenmiştir. 16S rDNA dizisinin moleküler analizleri, infekteli domates örneklerinde ‘Canditatus Phytoplasma solani’ (16SrXII-A grup) (benzerlik katsayısı 1.00) (Ulaşım no: MT279680) ve ‘Canditatus Phytoplasma trifolii’(16SrVI-A grup) (benzerlik katsayısı 1.00) (Ulaşım no: MT279852) doğrulamıştır. Domates fitoplazma ile ilişkili izolatlar ‘Bingöl D11’ diğerine ise ‘Bingöl D90’ izolatı olarak isimlendirilmiştir. Oluşturulan filogenetik dendrogram da her iki patojenin ait olduğu yeri doğrulamıştır.
Mevcut bu çalışma Bingöl ilinde domatesi doğal olarak enfekte eden "Ca. P. solani" ve "Ca. P. trifolii" nin ilk raporudur.

Keywords

“Ca. P. solani”,, “Ca. P. trifolii”,, Virtual RFLP,, 16S rRNA geni,, Domates

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