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Origanum onites L. ve Rosmarinus officinalis L. Uçucu Yağlarının Kimyasal İçerikleri ve Fusarium solani’ ye Karşı Antifungal Aktivitelerinin Belirlenmesi

Year 2021, Volume: 8 Issue: 2, 329 - 335, 23.04.2021
https://doi.org/10.30910/turkjans.825090

Abstract

Bu çalışma, kekik (Origanum onites L.) ve biberiye (Rosmarinus officinalis L.) bitkilerinden elde edilen uçucu yağların Fusarium solani‘ye karşı antifungal aktivitesini tespit etmek amacıyla laboratuvar ortamında yürütülmüştür. Bitkilerden elde edilen uçucu yağlar 1, 2 ve 4 μl dozunda otoklav edilen Patates Dekstroz Agar (PDA) besi yerine ilave edilmiştir. Patojenlere ait 8 mm çapında miselyum diskleri PDA besi yerlerine ekilmiştir. Kontrol grubu olarak uçucu yağlardan ari PDA besi yeri kullanılmıştır. PDA’lı petriler 24±1oC’da 7 gün inkübasyona bırakılmıştır. İnkübasyon sonrasında fungusların koloni çapları ölçülerek, kontrol grubuna göre bitki uçucu yağlarının % engelleme oranları hesaplanmıştır. Çalışmadan elde edilen verilere göre, Rosmarinus officinalis L. uçucu yağının yüksek dozu (4 μl) test edilen Fusarium solani’ye karşı %71,72 etki göstermiş ve doz seviyesi arttıkça antifungal etkinin de arttığı belirlenmiştir. Origanum onites L. uçucu yağı, uygulanan üç dozda Fusarium solani’nin misel gelişiminde %78,91-%82,70 oranları arasında engelleme göstermiştir. Çalışmada kekik ve biberiye uçucu yağları kontrol grubu ile karşılaştırıldığında Fusarium solani’ye karşı farklı seviyelerde engelleyici etkiler gösterdikleri tespit edilmiş ve patojenlerin koloni gelişimine en yüksek antifungal etki (%82,70) kekik uçucu yağının 2 μl ve 4 μl doz uygulamalarında saptanmıştır. Bu sonuçlar bitkilerden elde edilen uçucu yağların sentetik pestisitlere alternatif olabileceğini göstermiştir.

References

  • Alvarez-Castellanos, P.P., Bishop, C.D. ve Pascual-Villalobos, M.J. 2001. Antifungal activity of essential oil of flowerheads of garland chrysanthemum (Chrysanthemum coronarium) against agricultural pathogens. Phytochemistry, 57: 99-102.
  • Araniti, F., Mancuso, R., Lupini, A., Giofrè, S., Sunseri, F., Gabriele, B., Abenavoli, M. 2015. Phytotoxic potential and biological activity of three synthetic coumarin derivatives as new natural-like herbicides. Molecules, 20: 17883-17902. https:// doi.org/10.3390/molecules201017883
  • Baghloul, F., Mansori, R., Djahoudi, A. 2017. In vitro antifungal effect of Rosmarinus officinalis essential oil on Aspergillus niger. National Journal of Physiology, Pharmacy and Pharmacology, 7(3): 285-289.
  • Baruah, P., Sharma, R.K., Singh, R.S., Ghosh, A.C. 1996. Fungicidal Activity of Some Naturally Occurring Essential Oils Against Fusarium moniliforme. Journal of Essential Oil Research, 8(4): 411-412.
  • Bayar, Y., Yılar, M., Onaran, A. 2018. Antifungal activity of Origanum onites L. essential oil against some plant pathogenic fungi. IX International Scientific Agriculture Symposium "AGROSYM 2018", 4-7 October, Jahorina, Bosnia and Herzegovina, Book of Proceedings. pp.1265-1269.
  • Bayrak, A. ve Akgül, A., 1987. Composition of essential oils from Turkish Salvia species. Phytochemistry, 26: 846-847.
  • Ben Kaab, S., Rebey, I. B., Hanafi, M., Berhal, C., Fauconnier, M. L., Clerck, C. De, Ksouri, R., Jijakli, H. 2019. Rosmarinus officinalis essential oil as an effective antifungal and herbicidal agent. Spanish Journal of Agricultural Research, 17(2): 41.
  • Benjilali, B., Tantadui-Elaraki, A., Ayadi, A. ve Ihlal, M. 1984. Method to Study Antimicrobial Effects of Essential Oils: Application to the Antifungal Activity of Six Moroccan Essences. Journal of Food Protection, 47: 748-752.
  • Bhaskara Reddy, M.V., Angers, P., Gosselin, A., Arul, J. 1998. Characterization and use of essential oil from Thymus vulgaris against Botrytis cinerea and Rhizopus stolonifer in strawberry fruits. Phytochemistry, 47 (8): 1515-1520.
  • Bomfim, N. da S., Kohiyama, C.Y., Nakasugi, L.P., Nerilo, S.B., Mossini, S.A.G. ,Romoli, J.C.Z., Mikcha, J.M.G., Abreu Filho, B.A. de, Machinski, M. Jr. 2020. Antifungal and antiaflatoxigenic activity of rosemary essential oil (Rosmarinus officinalis L.) against Aspergillus flavus. Food Additives and Contaminants, 37(1): 153-161.
  • Boyraz, N. ve Özcan, M., 1997. Bitki patojeni funguslara bazı yerli baharat ekstrakt ve uçucu yağlarının antifungal etkileri. Gıda, 22(6): 457-462.
  • Boyraz, N., Koçak, R. 2006. Bazı bitki ekstraktlarının in vitro antifungal etkileri. Selçuk Üniversitesi Ziraat Fakültesi Dergisi, 20 (38): 82-87.
  • Centeno, S., Calvo M.A., Adelantado C. and Figueroa S. 2010 Antifungal Activity of Extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus. Pakistan Journal of Biological Sciences, 13(9): 452-5. DOI: 10.3923/pjbs.2010.452.455
  • Çakır, C., Yeğen, O. 1991. Antalya ve çevresindeki bazı bitkilerin ve uçucu yağlarının fungitoksik potansiyellerinin araştırılması. VI. Türkiye Fitopatoloji Kongresi, 7-11 Ekim, İzmir, s. 213-218.
  • Çalıkoğlu, E., Kıralan, M. ve Bayrak, A., 2006. Uçucu Yağ Nedir, Nasıl Üretilir ve Türkiye’deki Durumuna Genel Bir Bakış. Türkiye 9. Gıda Kongresi, 24-26 Mayıs, Bolu, s. 569-570.
  • Daferera, D.J., Ziogas, B.N., Polissiou, M.G. 2000. MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. J. Agric. Food Chem., 48 (6): 2576-2581.
  • Deans, S.G. ve Svoboda, K.P., 1990. The Antimicrobial Properties of Marjoram (Origanum majorana L.) Volatile Oil. Flavour Fragr. Journal, 5: 187-190.
  • Del Castillo, J.M.S., 2007. Micotoxinas en Alimentos. Madrid Press, Spain, pp 396. ISBN: 978-8479788087.
  • Duke, S.O., Dayan, F.E., Romagni, J.G. ve Rimando, A.M., 2000. Natural products as sources of herbicides: current status and future trends. Weed Research, 40: 99-111.
  • El-Sherbieny, S.N., Zakey, W.H., Abdel Ghafor, S.M. 2002. Antifungal action of some essential oils against fungi causing cotton seedling damping-off disease. Annals Agric. Sci., 47(3): 1009-1020.
  • Erdoğan, O., Çelik, A., Yıldız, Ş., Kökten, K. 2014. Pamukta fide kök çürüklüğü etmenlerine karşı bazı bitki ekstrakt ve uçucu yağlarının antifungal etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 1(3): 398-404.
  • Grover, R.K. and Moore, J.D. 1962. Toximetric studies of fungicides against brown rot organism, Sclerotina fruticola. Phytopathology, 52: 876-880.
  • Hashem, M., Moharama, A.M., Zaied, A.A. ve Saleh, F.E.M., 2010. Efficacy of essential oils in the control of cumin root rot disease caused by Fusarium spp. Crop Protection, 29: 1111-1117.
  • Kaçar, Ö. ve Özer, N. 2000. Soğanda Tohumla ve Toprakla Taşınan Funguslar Üzerine Bazı Bitki Ekstraktları ve Kompost Ekstraktları Uygulamalarının Etkinliği. Yüksek Lisans Tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Tekirdağ, s. 68.
  • Kordali, S., Usanmaz, A., Cakir, A., Komaki, A., Ercisli, S. 2016. Antifungal and herbicidal effects of fruit essential oils of four Myrtus communis genotypes. Chem Biodivers, 13 (1): 77-84. https://doi.org/10.1002/cbdv.201500018
  • Koul, O., Walia, S., Dhaliwal, G.S. 2008. Essential oils as green pesticides: Potential and constraints. Biopestic Int., 4: 63-84.
  • Lee, S.O., Choi, G.J., Jang, K.S., Lim, H.K., Cho, K.Y., Kim, J.C. 2007. Antifungal activity of five plant essential oils as fumigant against post-harvest and soilborne plant pathogenic fungi. The Plant Pathology Journal, 23:97-102.
  • Lehtijarvi-Doğmuş, H.T., 2006. Antifungal effect of essential oils from some Turkish herbs against Rhizoctonia solani Kühn. Phytopathology Mediterranean, 45: 261– 265.
  • Macias, F.A., Castellano, D., Oliva, R.M., Cross, P. ve Torres, A., 1997. Potential use of allelopathic agents as natural agrochemicals. Brighton Crop Protection Conference Weeds, p. 33-38.
  • Marotti, M., and Piccaglia, R. 1992. The Influence of Distillation Conditions on the Essential Oil Composition of Three Varieties of Foeniculum vulgare Mill. Journal of Essential Oil Research, 4: 569-576.
  • Qasem, J.R. ve Abu-Blan, H.A., 1995. Antifungal Activity of Aqueous Extract From Some Common Weed Species. Annals of Applied Biology, 127: 215-219.
  • Rathee, P.S., Mishra, S.H. ve Kaushal, R., 1982. Antimicrobial activity of essential oil, fixed oil and unsaponifiable matter of Nigella sativa Linn. Indian Journal of Pharmacy Science, 44: 8-10.
  • Schütte, G., Eckerstorfer, M., Rastelli, V., Reichenbecher, W., Restrepo-Vassalli, S., Ruohonen-Lehto, M., Saucy, A.G.W., Mertens, M. 2017. Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants. Environ.Sci.Eur. 29(1): 5. https://doi.org/10.1186/s12302-016-0100-y
  • Shelef, L.A., 1983. Antimicrobial effects of spices. Journal of Food Safety, 6: 29-44.
  • Singh, A.K., Dikshit, A., Sharma, M.L. ve Dixit, S.N., 1980. Fungitoxic Activity of Some Essential Oils. Economic Botany, 34:186-190.
  • Sevindik, E., Aydin, S., Kurtoglu, C., Tin, B. 2019.Evaluation of essential oil composition of Origanum onites L. (Lamiaceae) plant and antifungal activity on some strong pathogen fungi. Advances in Food Sciences, 41(2): 32-35.
  • Stein, S.E. 1990. National Institute of Standardsand Technology (NIST) Mass Spectral Database and Software, Version 3.02, Juen USA.
  • Thompson, C. and Henke, S.E. 2000. Effect of climate and type of storage container on aflatoxin production in corn and its associated risk to wildlife species. J. Wildl. Dis., 36: 172-179.
  • Walter, M., Jaspers, M.V., Eade, K., Frampton, C.M., Stewart, A. 2001. Control of Botrytis cinerea in grape using thyme oil. Australasian Plant Pathology, 30, 21-25.
  • Wilson, C.L., Solar, J.M., El Ghaouth, A., Wisniewski, M.E. 1997. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Diseases, 81(2): 204-210.
  • Zambonelli, A., Zechini D’Aulerio, A., Bianchi, A., Albasini, A. 1996. Effects of essential oils on phytopathogenic fungi in vitro. J. Phytopathol., 144(9/10): 491-494.
Year 2021, Volume: 8 Issue: 2, 329 - 335, 23.04.2021
https://doi.org/10.30910/turkjans.825090

Abstract

References

  • Alvarez-Castellanos, P.P., Bishop, C.D. ve Pascual-Villalobos, M.J. 2001. Antifungal activity of essential oil of flowerheads of garland chrysanthemum (Chrysanthemum coronarium) against agricultural pathogens. Phytochemistry, 57: 99-102.
  • Araniti, F., Mancuso, R., Lupini, A., Giofrè, S., Sunseri, F., Gabriele, B., Abenavoli, M. 2015. Phytotoxic potential and biological activity of three synthetic coumarin derivatives as new natural-like herbicides. Molecules, 20: 17883-17902. https:// doi.org/10.3390/molecules201017883
  • Baghloul, F., Mansori, R., Djahoudi, A. 2017. In vitro antifungal effect of Rosmarinus officinalis essential oil on Aspergillus niger. National Journal of Physiology, Pharmacy and Pharmacology, 7(3): 285-289.
  • Baruah, P., Sharma, R.K., Singh, R.S., Ghosh, A.C. 1996. Fungicidal Activity of Some Naturally Occurring Essential Oils Against Fusarium moniliforme. Journal of Essential Oil Research, 8(4): 411-412.
  • Bayar, Y., Yılar, M., Onaran, A. 2018. Antifungal activity of Origanum onites L. essential oil against some plant pathogenic fungi. IX International Scientific Agriculture Symposium "AGROSYM 2018", 4-7 October, Jahorina, Bosnia and Herzegovina, Book of Proceedings. pp.1265-1269.
  • Bayrak, A. ve Akgül, A., 1987. Composition of essential oils from Turkish Salvia species. Phytochemistry, 26: 846-847.
  • Ben Kaab, S., Rebey, I. B., Hanafi, M., Berhal, C., Fauconnier, M. L., Clerck, C. De, Ksouri, R., Jijakli, H. 2019. Rosmarinus officinalis essential oil as an effective antifungal and herbicidal agent. Spanish Journal of Agricultural Research, 17(2): 41.
  • Benjilali, B., Tantadui-Elaraki, A., Ayadi, A. ve Ihlal, M. 1984. Method to Study Antimicrobial Effects of Essential Oils: Application to the Antifungal Activity of Six Moroccan Essences. Journal of Food Protection, 47: 748-752.
  • Bhaskara Reddy, M.V., Angers, P., Gosselin, A., Arul, J. 1998. Characterization and use of essential oil from Thymus vulgaris against Botrytis cinerea and Rhizopus stolonifer in strawberry fruits. Phytochemistry, 47 (8): 1515-1520.
  • Bomfim, N. da S., Kohiyama, C.Y., Nakasugi, L.P., Nerilo, S.B., Mossini, S.A.G. ,Romoli, J.C.Z., Mikcha, J.M.G., Abreu Filho, B.A. de, Machinski, M. Jr. 2020. Antifungal and antiaflatoxigenic activity of rosemary essential oil (Rosmarinus officinalis L.) against Aspergillus flavus. Food Additives and Contaminants, 37(1): 153-161.
  • Boyraz, N. ve Özcan, M., 1997. Bitki patojeni funguslara bazı yerli baharat ekstrakt ve uçucu yağlarının antifungal etkileri. Gıda, 22(6): 457-462.
  • Boyraz, N., Koçak, R. 2006. Bazı bitki ekstraktlarının in vitro antifungal etkileri. Selçuk Üniversitesi Ziraat Fakültesi Dergisi, 20 (38): 82-87.
  • Centeno, S., Calvo M.A., Adelantado C. and Figueroa S. 2010 Antifungal Activity of Extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus. Pakistan Journal of Biological Sciences, 13(9): 452-5. DOI: 10.3923/pjbs.2010.452.455
  • Çakır, C., Yeğen, O. 1991. Antalya ve çevresindeki bazı bitkilerin ve uçucu yağlarının fungitoksik potansiyellerinin araştırılması. VI. Türkiye Fitopatoloji Kongresi, 7-11 Ekim, İzmir, s. 213-218.
  • Çalıkoğlu, E., Kıralan, M. ve Bayrak, A., 2006. Uçucu Yağ Nedir, Nasıl Üretilir ve Türkiye’deki Durumuna Genel Bir Bakış. Türkiye 9. Gıda Kongresi, 24-26 Mayıs, Bolu, s. 569-570.
  • Daferera, D.J., Ziogas, B.N., Polissiou, M.G. 2000. MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. J. Agric. Food Chem., 48 (6): 2576-2581.
  • Deans, S.G. ve Svoboda, K.P., 1990. The Antimicrobial Properties of Marjoram (Origanum majorana L.) Volatile Oil. Flavour Fragr. Journal, 5: 187-190.
  • Del Castillo, J.M.S., 2007. Micotoxinas en Alimentos. Madrid Press, Spain, pp 396. ISBN: 978-8479788087.
  • Duke, S.O., Dayan, F.E., Romagni, J.G. ve Rimando, A.M., 2000. Natural products as sources of herbicides: current status and future trends. Weed Research, 40: 99-111.
  • El-Sherbieny, S.N., Zakey, W.H., Abdel Ghafor, S.M. 2002. Antifungal action of some essential oils against fungi causing cotton seedling damping-off disease. Annals Agric. Sci., 47(3): 1009-1020.
  • Erdoğan, O., Çelik, A., Yıldız, Ş., Kökten, K. 2014. Pamukta fide kök çürüklüğü etmenlerine karşı bazı bitki ekstrakt ve uçucu yağlarının antifungal etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 1(3): 398-404.
  • Grover, R.K. and Moore, J.D. 1962. Toximetric studies of fungicides against brown rot organism, Sclerotina fruticola. Phytopathology, 52: 876-880.
  • Hashem, M., Moharama, A.M., Zaied, A.A. ve Saleh, F.E.M., 2010. Efficacy of essential oils in the control of cumin root rot disease caused by Fusarium spp. Crop Protection, 29: 1111-1117.
  • Kaçar, Ö. ve Özer, N. 2000. Soğanda Tohumla ve Toprakla Taşınan Funguslar Üzerine Bazı Bitki Ekstraktları ve Kompost Ekstraktları Uygulamalarının Etkinliği. Yüksek Lisans Tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Tekirdağ, s. 68.
  • Kordali, S., Usanmaz, A., Cakir, A., Komaki, A., Ercisli, S. 2016. Antifungal and herbicidal effects of fruit essential oils of four Myrtus communis genotypes. Chem Biodivers, 13 (1): 77-84. https://doi.org/10.1002/cbdv.201500018
  • Koul, O., Walia, S., Dhaliwal, G.S. 2008. Essential oils as green pesticides: Potential and constraints. Biopestic Int., 4: 63-84.
  • Lee, S.O., Choi, G.J., Jang, K.S., Lim, H.K., Cho, K.Y., Kim, J.C. 2007. Antifungal activity of five plant essential oils as fumigant against post-harvest and soilborne plant pathogenic fungi. The Plant Pathology Journal, 23:97-102.
  • Lehtijarvi-Doğmuş, H.T., 2006. Antifungal effect of essential oils from some Turkish herbs against Rhizoctonia solani Kühn. Phytopathology Mediterranean, 45: 261– 265.
  • Macias, F.A., Castellano, D., Oliva, R.M., Cross, P. ve Torres, A., 1997. Potential use of allelopathic agents as natural agrochemicals. Brighton Crop Protection Conference Weeds, p. 33-38.
  • Marotti, M., and Piccaglia, R. 1992. The Influence of Distillation Conditions on the Essential Oil Composition of Three Varieties of Foeniculum vulgare Mill. Journal of Essential Oil Research, 4: 569-576.
  • Qasem, J.R. ve Abu-Blan, H.A., 1995. Antifungal Activity of Aqueous Extract From Some Common Weed Species. Annals of Applied Biology, 127: 215-219.
  • Rathee, P.S., Mishra, S.H. ve Kaushal, R., 1982. Antimicrobial activity of essential oil, fixed oil and unsaponifiable matter of Nigella sativa Linn. Indian Journal of Pharmacy Science, 44: 8-10.
  • Schütte, G., Eckerstorfer, M., Rastelli, V., Reichenbecher, W., Restrepo-Vassalli, S., Ruohonen-Lehto, M., Saucy, A.G.W., Mertens, M. 2017. Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants. Environ.Sci.Eur. 29(1): 5. https://doi.org/10.1186/s12302-016-0100-y
  • Shelef, L.A., 1983. Antimicrobial effects of spices. Journal of Food Safety, 6: 29-44.
  • Singh, A.K., Dikshit, A., Sharma, M.L. ve Dixit, S.N., 1980. Fungitoxic Activity of Some Essential Oils. Economic Botany, 34:186-190.
  • Sevindik, E., Aydin, S., Kurtoglu, C., Tin, B. 2019.Evaluation of essential oil composition of Origanum onites L. (Lamiaceae) plant and antifungal activity on some strong pathogen fungi. Advances in Food Sciences, 41(2): 32-35.
  • Stein, S.E. 1990. National Institute of Standardsand Technology (NIST) Mass Spectral Database and Software, Version 3.02, Juen USA.
  • Thompson, C. and Henke, S.E. 2000. Effect of climate and type of storage container on aflatoxin production in corn and its associated risk to wildlife species. J. Wildl. Dis., 36: 172-179.
  • Walter, M., Jaspers, M.V., Eade, K., Frampton, C.M., Stewart, A. 2001. Control of Botrytis cinerea in grape using thyme oil. Australasian Plant Pathology, 30, 21-25.
  • Wilson, C.L., Solar, J.M., El Ghaouth, A., Wisniewski, M.E. 1997. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Diseases, 81(2): 204-210.
  • Zambonelli, A., Zechini D’Aulerio, A., Bianchi, A., Albasini, A. 1996. Effects of essential oils on phytopathogenic fungi in vitro. J. Phytopathol., 144(9/10): 491-494.
There are 41 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Işıl Saraç Sivrikaya 0000-0002-5991-2173

Bekir Tosun 0000-0002-2470-3865

Ersin Karakaya 0000-0002-6734-4962

Publication Date April 23, 2021
Submission Date November 13, 2020
Published in Issue Year 2021 Volume: 8 Issue: 2

Cite

APA Saraç Sivrikaya, I., Tosun, B., & Karakaya, E. (2021). Origanum onites L. ve Rosmarinus officinalis L. Uçucu Yağlarının Kimyasal İçerikleri ve Fusarium solani’ ye Karşı Antifungal Aktivitelerinin Belirlenmesi. Türk Tarım Ve Doğa Bilimleri Dergisi, 8(2), 329-335. https://doi.org/10.30910/turkjans.825090