Effects of Supplementation With Iron, Selenium and Magnesium on Galleria mellonella Cell-Mediated Immune Responses

Year 2021, Volume: 8 Issue: 3, 605 - 614, 26.07.2021

Serhat Kaya , Bülent Gündüz

https://doi.org/10.30910/turkjans.911913

Abstract

Immune responses play a key role in the survival of all living things. Intake of inorganic materials is of critical importance in living organisms because of their participation in metabolism and some component of cells. Iron (Fe), magnesium (Mg) and selenium (Se) were selected for this study because of their participation in critical constituents of cells, such as nucleic acids and enzymes in living organisms, and their indirect and direct effects on immunity. Although the separate effects of these inorganic materials are known, there is no study on their combined effects. Galleria mellonella, which is used as a model organism, is frequently used in evaluating of effects of human pathogens due to its various properties. Total hemocyte counts and encapsulation-melanization responses are the most commonly observed parameters when examining the immune responses of this model organism. In our study, the effects of the selected inorganic materials added in the ratio of 0.1%, 0.25%, 0.5% and 1% in the nutrient content of Galleria mellonella on the immune responses of this organism were investigated. As a result of the study, it was found that only 0.25% of the doses increased the hemocyte count and that the other doses significantly decreased compared to the control group. However, it was determined that 0.5% and 1% feeding caused a significant decrease in both hemocyte count and encapsulation-melanization responses. According to our findings, the nutrients which have over a certain dose by inorganic substances have negative effects on immunity.

Keywords

Galleria mellonella, Fe, Se, Mg, encapsulation-melanization

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi BAP birimi

Project Number

FBA-2018-2521

Thanks

This work was supported by The Scientific Research Coordination Unit of Çanakkale Onsekiz Mart University, Turkey (Project number: FBA-2018-2521).

Demir, Selenyum ve Magnezyum ile Takviyenin Galleria mellonella Hücre Aracılı Bağışıklık Tepkileri Üzerindeki Etkileri

Abstract

Bağışıklık tepkileri, tüm canlıların hayatta kalmasında önemli bir rol oynar. İnorganik materyallerin bünyeye alımı; canlı organizmalarda metabolizmaya ve bazı hücrelerin bileşenlerine katılımları nedeniyle kritik öneme sahiptir. Bu çalışmada demir (Fe), magnezyum (Mg) ve selenyum (Se) canlı organizmalardaki nükleik asitler ve enzimler gibi hücrelerin kritik bileşenlerine katılımları ve bağışıklık üzerindeki dolaylı ve doğrudan etkileri nedeniyle seçilmiştir. Bu inorganik maddelerin ayrı ayrı etkileri bilinmesine rağmen, bunların birleşik etkileri üzerine bir çalışma bulunmamaktadır. Model organizma olarak kullanılan Galleria mellonella, çeşitli özelliklerinden dolayı insan patojenlerinin etkilerinin değerlendirilmesinde sıklıkla kullanılmaktadır. Toplam hemosit sayımları, kapsülleme-melanizasyon tepkileri, bu model organizmanın bağışıklık tepkilerini incelerken en sık gözlemlenen parametrelerdir. Çalışmamızda; Galleria mellonella'nın besin içeriğine% 0.1,% 0.25,% 0.5 ve% 1 oranında eklenen seçilmiş inorganik maddelerin bu organizmanın immün yanıtları üzerindeki etkileri araştırılmıştır. Çalışma sonucunda kontrol grubuna göre dozların sadece% 0.25'inin hemosit sayısını artırdığı, diğer dozların ise önemli ölçüde azaldığı tespit edildi. Bununla birlikte,% 0.5 ve% 1 beslemenin hem hemosit sayısında hem de kapsülleme-melanizasyon yanıtlarında önemli bir düşüşe neden olduğu belirlendi. Bu çalışmanın sonuçlarından biri, bu inorganik maddelerin besin içeriğinin% 2'sinin üzerine eklendiğinde gelişimini durdurması ve daha yüksek dozların larvaların ölümüne neden olmasıdır. Bulgularımıza göre inorganik maddeler tarafından belirli bir dozun üzerinde bulunan besinler bağışıklık üzerinde olumsuz etkilere sahiptir.

Keywords

Galleria mellonella, Fe, Se, Mg, enkapsülasyon-melanizasyon

Project Number

FBA-2018-2521

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