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Siirt ve Şanlıurfa’da Bulunan Bal arılarında Nosemosis Etkenlerinin Moleküler Teşhisi

Year 2021, Volume: 8 Issue: 4, 995 - 1000, 24.10.2021
https://doi.org/10.30910/turkjans.927169

Abstract

Nosemosis bal arılarında (Epis mellifera) sıkça görülen küresel bir hastalıktır. Hastalığın etkeni fungal mikroorganizmalar olan Nosema apis ve Nosema ceranae’dir. Bu çalışmada Siirt ve Şanlıurfa’da bal verimi düşük ve zayıf (popülasyonu düşük) arı kolonilerinde Nosema spp. sporlarının varlığının mikroskobik olarak muayene edilmesi ve pozitif bulunan örneklerden, multipleks PCR (Polimeraz zincir reaksiyonu) ile etkenin tür düzeyinde tespiti amaçlandı. Her iki ilde toplam 8 arılıkta 44 kovandan alınan 880 arı numunesi mikroskobik olarak spor varlığı yönünden incelenmiş ve 19’unda (%43,18) nosema sporları tespit edilmiştir. Multipleks PCR ile bu sporların tamamının N. ceranae olduğu belirlendi.
Sonuç olarak, nosemosis’in bölgede arıcılık işletmelerinde önemli bir problem olduğu, bu nedenle nosemosis yönünden gerekli tedbirlerin alınması gerektiği kanısına varıldı.

Supporting Institution

Harran Üniversitesi Bilimsel Araştırma Projeleri (BAP) Komisyonu

Project Number

18079

References

  • Abacı, N.İ., Abacı, S.H., Bıyık, S. 2020. Forecast for the Number of Colonies and Honey Yield in Turkey. Turkish Journal of Agriculture-Food Science and Technology, 8(2), 464-470.
  • Anonim, 2021. Türkiye İstatistik Kurumu (TÜİK),Hayvansal Üretim İstatistikleri, Aralık 2020. https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-Aralik-2020-37207 (erişim tarihi: 22.04.2021).
  • Büyük, M., Tunca, R.İ., Taşkın, A. 2014. Türkiye’de Nosema spp. Varlığına Yönelik Yapılmış Çalışmalar. Türk Tarım ve Doğa Bilimleri Dergisi, 1(2), 234-238.
  • Büyük, M., Tunca, R.İ., Taşkın, A. 2017. Kırşehir İlindeki Arılıklarda Nosema Hastalığının Belirlenmesi. Türk Tarım-Gıda Bilim ve Teknoloji dergisi, 5(1), 1-5.
  • Chemurot, M., De Smet, L., Brunain, M., De Rycke, R., de Graaf, D.C. 2017. Nosema neumanni n. sp.(Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda. European journal of protistology, 61, 13-19.
  • Duquesne, V., Gastaldi, C., Del Cont, A., Cougoule, N., Bober, A., Brunain, M., ... & Franco, S. 2021. An international inter-laboratory study on Nosema spp. spore detection and quantification through microscopic examination of crushed honey bee abdomens. Journal of Microbiological Methods, 184, 106183.
  • Forsgren, E., Fries, I. 2010. Comparative virulence of Nosema ceranae and Nosema apis in individual European honey bees. Veterinary parasitology, 170(3-4), 212-217.
  • Fries, I., Martin, R., Meana, A., García-Palencia, P., Higes, M. 2006. Natural infections of Nosema ceranae in European honey bees. Journal of Apicultural Research, 45(4), 230-233.
  • Grupe, A.C., Quandt, C.A. 2020. A growing pandemic: A review of Nosema parasites in globally distributed domesticated and native bees. PLoS pathogens, 16(6), e1008580.
  • Hamiduzzaman, M.M., Guzman-Novoa, E., Goodwin, P.H. 2010. A multiplex PCR assay to diagnose and quantify Nosema infections in honey bees (Apis mellifera). Journal of Invertebrate Pathology, 105(2), 151-155.
  • Han, B., Weiss, L. M. (2017). Microsporidia: obligate intracellular pathogens within the fungal kingdom. The fungal kingdom, 97-113.
  • Hges, M., Martín-Hernández, R., Meana, A. 2010. Nosema ceranae in Europe: an emergent type C nosemosis. Apidologie, 41(3), 375-392.
  • Higes, M., Martín, R., Meana, A. (2006). Nosema ceranae, a new microsporidian parasite in honeybees in Europe. Journal of invertebrate pathology, 92(2), 93-95.
  • Huang, W.F., Solter, L.F. 2013. Comparative development and tissue tropism of Nosema apis and Nosema ceranae. Journal of invertebrate pathology, 113(1), 35-41.
  • Kartal, S., Tunca, R. İ., Özgül, O., Karabağ, K., Koç, H. 2021. Microscopic and molecular detection of nosema sp. in the Southwest Aegean Region. Uludağ Arıcılık Dergisi, 21(1), 8-20.
  • Martín‐Hernández, R., Bartolomé, C., Chejanovsky, N., Le Conte, Y., Dalmon, A., Dussaubat, C., Garcia-Palencia, P., Meana, A., Pinto, M.A., Soroker, V., Higes, M. 2018. Nosema ceranae in Apis mellifera: a 12 years postdetection perspective. Environmental microbiology, 20(4), 1302-1329.7.
  • Martín-Hernández, R., Meana, A., Prieto, L., Salvador, A.M., Garrido-Bailón, E., Higes, M. 2007. Outcome of colonization of Apis mellifera by Nosema ceranae. Applied and environmental microbiology, 73(20), 6331-6338.
  • Mohammadian, B., Bokaie, S., Moharrami, M., Nabian, S., Forsi, M. 2018. Distribution of Nosema Spp. in climatic regions of Iran. In Veterinary Research Forum (Vol. 9, No. 3, p. 259). Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
  • OIE, Terrestrial Manual 2008. Nosemosis of honey bees. In: Manual of Diagnostic Tests and Vacines for Terrestrial Animals, Chapter 2.2.4, pp. 410-414.
  • Shumkova, R., Georgieva, A., Radoslavov, G., Sirakova, D., Dzhebir, G., Neov, B., Bouga M., Hristov, P. 2018. The first report of the prevalence of Nosema ceranae in Bulgaria. PeerJ, 6, e4252.
  • Sinpoo, C., Paxton, R.J., Disayathanoowat, T., Krongdang, S., Chantawannakul, P. 2018. Impact of Nosema ceranae and Nosema apis on individual worker bees of the two host species (Apis cerana and Apis mellifera) and regulation of host immune response. Journal of insect physiology, 105, 1-8.
  • Szalanski, A.L., Tripodi, A.D., Trammel, C.E. 2014. Molecular detection of Nosema apis and N. ceranae from southwestern and south central USA feral Africanized and European honey bees, Apis mellifera (Hymenoptera: Apidae). Florida Entomologist, 97(2), 585-589.
  • Tosun, O., Bekircan, Ç., Yıldırım, H.B. 2019. The Presence and Distribution of Nosemosis Disease in Turkey. Apiterapi ve Doğa Dergisi, 2(2), 71-84.
  • Tunca, R.I., Oskay, D., Gosterit, A., Tekin, O.K. 2016. Does Nosema ceranae wipe out Nosema apis in Turkey?. Iranian journal of parasitology, 11(2), 259.
  • Ütük, A.E., Aliyeva, R., Girisgin, A.O., Gökmen, T.G., Özüiçli, M., Aydın, L. 2019. First molecular detection of Nosema ceranae in Azerbaijan. Journal of Apicultural Research, 58(4), 559-561.
  • Ütük, A.E., Pişkin, F.Ç., Kurt, M. 2010. Türkiye’de Nosema ceranae’nın ilk moleküler tanısı. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 57, 275-278, 2010.
  • Whitaker, J., Szalanski, A.L., Kence, M. 2011. Molecular detection of Nosema ceranae and N. apis from Turkish honey bees. Apidologie, 42(2), 174-180.
  • Yaman, M., Yarılgaç, E.Ş., Güner, B.G., Ertürk, Ö. 2015. Ordu yöresi bal arılarında Nosemosis hastalığının varlığı ve dağılımı. Turkiye Parazitoloji Dergisi, 39, 47-51.

Molecular Identification of Nosemosis Agents in Honeybees Found in Siirt and Sanliurfa

Year 2021, Volume: 8 Issue: 4, 995 - 1000, 24.10.2021
https://doi.org/10.30910/turkjans.927169

Abstract

disease are Nosema apis and Nosema ceranae, which are fungal microorganisms. In this study, we aimed to detect the disease agent at species level in bee colonies in which honey production was poor ( low population) in Siirt and Şanlıurfa. For this purpose, the presence of spores of Nosema spp. was examined microscopically and positive samples were tested by multiplex polymerase reaction. A total of 880 bee samples from 8 apiaries and 44 hives in both provinces were examined microscopically and 19 out of 44 hives (43,18%) were found as positive for the presence of spores of Nosema. All the spores were belong to N. ceranae by multiplex PCR.
As conclusion, we thought that nosemosis is an important problem in honey bees apiaries in Siirt and Şanlıurfa and in this context, required prevention measures should be taken for nosemosis.

Project Number

18079

References

  • Abacı, N.İ., Abacı, S.H., Bıyık, S. 2020. Forecast for the Number of Colonies and Honey Yield in Turkey. Turkish Journal of Agriculture-Food Science and Technology, 8(2), 464-470.
  • Anonim, 2021. Türkiye İstatistik Kurumu (TÜİK),Hayvansal Üretim İstatistikleri, Aralık 2020. https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-Aralik-2020-37207 (erişim tarihi: 22.04.2021).
  • Büyük, M., Tunca, R.İ., Taşkın, A. 2014. Türkiye’de Nosema spp. Varlığına Yönelik Yapılmış Çalışmalar. Türk Tarım ve Doğa Bilimleri Dergisi, 1(2), 234-238.
  • Büyük, M., Tunca, R.İ., Taşkın, A. 2017. Kırşehir İlindeki Arılıklarda Nosema Hastalığının Belirlenmesi. Türk Tarım-Gıda Bilim ve Teknoloji dergisi, 5(1), 1-5.
  • Chemurot, M., De Smet, L., Brunain, M., De Rycke, R., de Graaf, D.C. 2017. Nosema neumanni n. sp.(Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda. European journal of protistology, 61, 13-19.
  • Duquesne, V., Gastaldi, C., Del Cont, A., Cougoule, N., Bober, A., Brunain, M., ... & Franco, S. 2021. An international inter-laboratory study on Nosema spp. spore detection and quantification through microscopic examination of crushed honey bee abdomens. Journal of Microbiological Methods, 184, 106183.
  • Forsgren, E., Fries, I. 2010. Comparative virulence of Nosema ceranae and Nosema apis in individual European honey bees. Veterinary parasitology, 170(3-4), 212-217.
  • Fries, I., Martin, R., Meana, A., García-Palencia, P., Higes, M. 2006. Natural infections of Nosema ceranae in European honey bees. Journal of Apicultural Research, 45(4), 230-233.
  • Grupe, A.C., Quandt, C.A. 2020. A growing pandemic: A review of Nosema parasites in globally distributed domesticated and native bees. PLoS pathogens, 16(6), e1008580.
  • Hamiduzzaman, M.M., Guzman-Novoa, E., Goodwin, P.H. 2010. A multiplex PCR assay to diagnose and quantify Nosema infections in honey bees (Apis mellifera). Journal of Invertebrate Pathology, 105(2), 151-155.
  • Han, B., Weiss, L. M. (2017). Microsporidia: obligate intracellular pathogens within the fungal kingdom. The fungal kingdom, 97-113.
  • Hges, M., Martín-Hernández, R., Meana, A. 2010. Nosema ceranae in Europe: an emergent type C nosemosis. Apidologie, 41(3), 375-392.
  • Higes, M., Martín, R., Meana, A. (2006). Nosema ceranae, a new microsporidian parasite in honeybees in Europe. Journal of invertebrate pathology, 92(2), 93-95.
  • Huang, W.F., Solter, L.F. 2013. Comparative development and tissue tropism of Nosema apis and Nosema ceranae. Journal of invertebrate pathology, 113(1), 35-41.
  • Kartal, S., Tunca, R. İ., Özgül, O., Karabağ, K., Koç, H. 2021. Microscopic and molecular detection of nosema sp. in the Southwest Aegean Region. Uludağ Arıcılık Dergisi, 21(1), 8-20.
  • Martín‐Hernández, R., Bartolomé, C., Chejanovsky, N., Le Conte, Y., Dalmon, A., Dussaubat, C., Garcia-Palencia, P., Meana, A., Pinto, M.A., Soroker, V., Higes, M. 2018. Nosema ceranae in Apis mellifera: a 12 years postdetection perspective. Environmental microbiology, 20(4), 1302-1329.7.
  • Martín-Hernández, R., Meana, A., Prieto, L., Salvador, A.M., Garrido-Bailón, E., Higes, M. 2007. Outcome of colonization of Apis mellifera by Nosema ceranae. Applied and environmental microbiology, 73(20), 6331-6338.
  • Mohammadian, B., Bokaie, S., Moharrami, M., Nabian, S., Forsi, M. 2018. Distribution of Nosema Spp. in climatic regions of Iran. In Veterinary Research Forum (Vol. 9, No. 3, p. 259). Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
  • OIE, Terrestrial Manual 2008. Nosemosis of honey bees. In: Manual of Diagnostic Tests and Vacines for Terrestrial Animals, Chapter 2.2.4, pp. 410-414.
  • Shumkova, R., Georgieva, A., Radoslavov, G., Sirakova, D., Dzhebir, G., Neov, B., Bouga M., Hristov, P. 2018. The first report of the prevalence of Nosema ceranae in Bulgaria. PeerJ, 6, e4252.
  • Sinpoo, C., Paxton, R.J., Disayathanoowat, T., Krongdang, S., Chantawannakul, P. 2018. Impact of Nosema ceranae and Nosema apis on individual worker bees of the two host species (Apis cerana and Apis mellifera) and regulation of host immune response. Journal of insect physiology, 105, 1-8.
  • Szalanski, A.L., Tripodi, A.D., Trammel, C.E. 2014. Molecular detection of Nosema apis and N. ceranae from southwestern and south central USA feral Africanized and European honey bees, Apis mellifera (Hymenoptera: Apidae). Florida Entomologist, 97(2), 585-589.
  • Tosun, O., Bekircan, Ç., Yıldırım, H.B. 2019. The Presence and Distribution of Nosemosis Disease in Turkey. Apiterapi ve Doğa Dergisi, 2(2), 71-84.
  • Tunca, R.I., Oskay, D., Gosterit, A., Tekin, O.K. 2016. Does Nosema ceranae wipe out Nosema apis in Turkey?. Iranian journal of parasitology, 11(2), 259.
  • Ütük, A.E., Aliyeva, R., Girisgin, A.O., Gökmen, T.G., Özüiçli, M., Aydın, L. 2019. First molecular detection of Nosema ceranae in Azerbaijan. Journal of Apicultural Research, 58(4), 559-561.
  • Ütük, A.E., Pişkin, F.Ç., Kurt, M. 2010. Türkiye’de Nosema ceranae’nın ilk moleküler tanısı. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 57, 275-278, 2010.
  • Whitaker, J., Szalanski, A.L., Kence, M. 2011. Molecular detection of Nosema ceranae and N. apis from Turkish honey bees. Apidologie, 42(2), 174-180.
  • Yaman, M., Yarılgaç, E.Ş., Güner, B.G., Ertürk, Ö. 2015. Ordu yöresi bal arılarında Nosemosis hastalığının varlığı ve dağılımı. Turkiye Parazitoloji Dergisi, 39, 47-51.
There are 28 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Osman Yaşar Tel 0000-0001-7848-3899

Songül Ötkün 0000-0003-2736-953X

Ayfer Güllü Yücetepe 0000-0002-9842-3305

Sevil Erdenliğ Gürbilek 0000-0002-0377-2650

Oktay Keskin 0000-0002-5977-7872

Project Number 18079
Publication Date October 24, 2021
Submission Date April 24, 2021
Published in Issue Year 2021 Volume: 8 Issue: 4

Cite

APA Tel, O. Y., Ötkün, S., Güllü Yücetepe, A., Erdenliğ Gürbilek, S., et al. (2021). Siirt ve Şanlıurfa’da Bulunan Bal arılarında Nosemosis Etkenlerinin Moleküler Teşhisi. Türk Tarım Ve Doğa Bilimleri Dergisi, 8(4), 995-1000. https://doi.org/10.30910/turkjans.927169