In vitro micropropagation and flowering of the endemic plant Linaria genistifolia (L.) Miller ssp. praealta (Boiss.) Davis

Year 2022, Volume: 9 Issue: 3, 628 - 637, 23.07.2022

Esra Bulunuz Palaz Selay Doğan Gülat Çağlar

Abstract

Linaria known as “nevruz otu” has a wide natural distribution area in Anatolia. The Linaria plant, which has been widely used in folk medicine in Anatolia for many years, has the potential to be used as ornamental plant. Biotechnological methods are valuable tools in the mass propagation of the plants grown in the nature. Tissue culture is widely used to propagation of medicinal plants for active compounds. Also, in vitro techniques serves protect to genetic resources especially endemic species. The aim of this study was to make rapid propagation of endemic plant of Linaria genistifolia (L.) Miller ssp. praealta (Boiss.) Davis, native to Anatolia. Plant growth regulators TDZ and BAP were added into ½ MS medium alone or in combination with GA3 to improve shoot propagation. The effect of plant growth regulators on shoot induction, rate of shoot, shoot number per explant and shoot length were determined. Considering both the shoot length and the number of shoots of in vitro propagation of Linaria genistifolia (L.) Miller ssp. praealta (Boiss.) Davis plants, it was concluded that the best results (32.6 shoots per explant) for obtaining plantlets could be achieved by adding 0.2 mg/l TDZ into the ½ MS medium. The highest shoot length was 41.4 mm on the media containing 0.5 mg/l BA which was closely followed by control treatment (34.6 mm). Healthy root development with shoot growth was observed in all media tested. Plantlets were grown on a mixture of peat:perlite in a 3:1 ratio. Rooted plants were successfully transferred to a greenhouse. 80 % of the plants survived and acclimatized in ex vitro conditions.

Keywords

in vitro propagation, endemic plants, Linaria genistifolia

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