Muscari armeniacum Bitkisinin Farklı Ekstraktlarının Antioksidan, Antimikrobiyal ve Sitotoksik Aktivitelerinin İncelenmesi

Year 2025, Volume: 12 Issue: 1, 187 - 196

Turgut Taşkın , Esra Avcı , Erkan Rayaman , Beyza Nur Yılmaz , Saliha Aydın , Ayşe Mine Yılmaz , Gizem Emre

Abstract

Bu çalışmanın amacı Muscari armeniacum bitkisinin toprak üstü ve soğanlarının maserasyon yöntemiyle elde edilen farklı ekstraktların antioksidan ve antimikrobiyal aktivitelerini karşılaştırmalı olarak değerlendirmektir. Ayrıca çalışma, etanol ekstraktlarının insan fibroblastlarının ve MCF-7 meme kanseri hücre hatlarının çoğalması üzerindeki etkisini belirlemeyi amaçlamaktadır. Muscari armeniacum'un toprak üstü kısımlarından ve soğanlarından elde edilen petrol eteri, kloroform ve etanol ekstraktlarının antioksidan aktiviteleri DPPH, CUPRAC ve FRAP yöntemleriyle incelenmiştir. Ekstraktların içerdiği toplam fenolik bileşik miktarı FCR yöntemi kullanılarak belirlendi. Antimikrobiyal aktivite agar kuyusu difüzyon yöntemiyle belirlendi. Bitkinin etanol ekstraktlarının sitotoksik aktiviteye yeteneği MTT tahlili kullanılarak belirlendi. Soğanların kloroform ekstraktının diğer ekstraktlara göre daha yüksek DPPH (IC50:0,056 mg/mL), FRAP (2,738 mM FeSO4/mg ekstrakt) ve CUPRAC (3,640 mM trolox eşdeğeri/mg ekstrakt) aktivite potansiyeline sahip olduğu belirlendi. Ayrıca toprak üstü kısım kloroform ekstraktının en yüksek DPPH (IC50: 0,889 mg/mL), CUPRAC (1,166 mM trolox eşdeğeri/mg ekstrakt) ve FRAP (0,823 mM FeSO4/mg ekstrakt aktivitesi) antioksidan aktivitesini gösterdiği belirlendi. Soğanlardan elde edilen kloroform ekstraktının toprak üstü kısımlara göre daha yüksek miktarda fenolik içerik içerdiği ve buna bağlı olarak en yüksek antioksidan aktiviteyi gösterdiği belirlendi. Hava parçaları petrol eteri ekstraktının S. aureus ATCC 25923 ve S. epidermidis ATCC 11228'e karşı antimikrobiyal aktiviteye sahip olduğu, soğanların etanol ekstraktının ise yalnızca S. epidermidis ATCC 11228'e karşı antimikrobiyal aktiviteye sahip olduğu bulundu.
Çalışma, 500 µg/mL dozundaki hava parçaları ve soğan etanol ekstraktlarının insan fibroblastları ve MCF-7 meme kanseri hücre dizileri üzerinde sitotoksik potansiyeli olmadığını gösterdi. Bitkinin farklı kısımlarının insan normal hücre hatları üzerinde sitotoksik aktivite göstermemesi, bu türün antioksidan ve antimikrobiyal ajan olarak kullanılabileceğini düşündürmektedir.

Keywords

Muscari armeniacum, antimikrobiyal, antioksidan, sitotoksik

Ethical Statement

Gerekli Değildir.

Supporting Institution

Tübitak

Project Number

1919B012209006

Thanks

This study was supported by The Scientific and Technological Research Council of Turkey (2209/A, Project no: 1919B012209006).

Investigation of Antioxidant, Antimicrobial and Cytotoxic Activities of Different Extracts of Muscari armeniacum

Abstract

The aim of this study is to comparatively evaluate the antioxidant and antimicrobial activities of different extracts obtained by the maceration method of the aerial and bulbs of the Muscari armeniacum. Additionally, the study aims to determine the effect of ethanol extracts on the proliferation of human fibroblasts and MCF-7 breast cancer cell lines. The antioxidant activities of petroleum ether, chloroform and ethanol extracts from the aerial parts and bulbs of the Muscari armeniacum were examined by DPPH, CUPRAC and FRAP methods. The amount of total phenolic compounds contained in the extracts was determined using the FCR method. Antimicrobial activity was determined by the agar well diffusion method. The ability ethanol extracts of plant to cytotoxic activity was determined using the MTT assay. It was determined that the chloroform extract of bulbs had higher DPPH (IC50:0.056 mg/mL), FRAP (2.738 mM FeSO4/mg extract) and CUPRAC (3.640 mM trolox equivalent/mg extract) activity potential than other extracts. In addition, it was determined that the aerial parts chloroform extract showed the highest DPPH (IC50: 0.889 mg/mL), CUPRAC (1.166 mM trolox equivalent/mg extract) and FRAP (0.823 mM FeSO4/mg extract activities) antioxidant activity. It was determined that the chloroform extract obtained from bulbs contained higher amounts of phenolic contents than the aerial parts and accordingly showed the highest antioxidant activity. Aerial parts petroleum ether extract was found to have antimicrobial activity against S. aureus ATCC 25923 and S. epidermidis ATCC 11228, and the ethanol extract of bulbs was found to have antimicrobial activity only against S. epidermidis ATCC 11228.
The study showed no cytotoxic potential of aerial parts and bulbs ethanol extracts at a dose of 500 µg/mL on human fibroblasts and MCF-7 breast cancer cell lines. The fact that different parts of the plant do not show cytotoxic activity on human normal cell lines suggests that this species can be used as an antioxidant and antimicrobial agent.

Keywords

Muscari armeniacum, antimicrobial, antioxidant, cytotoxic

Project Number

1919B012209006

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