Synergistic Effects of Cyanus depressus and Schizochytrium sp. Extracts on Growth Indices and The Expression Levels of Antioxidant and Immunity-Related Genes in Common Carp (Cyprinus carpio)

Year 2025, Volume: 12 Issue: 1, 111 - 121

Boran Karataş

Abstract

This study aimed to assess the combined effects of Cyanus depressus and Schizochytrium sp. extracts on growth indices and the expression of genes linked to antioxidant activity and immunity in carp (Cyprinus carpio). Fish, averaging 3.71 ± 0.16 g in weight, were assigned to three dietary groups over a 60-day period: a control group (0 g/kg extract) and two test groups receiving extract mixtures at 0.5 g/kg (CS05) and 1 g/kg (CS1). The supplemented groups, particularly those at 0.5 and 1 g/kg, showed significant increases in final body weight, weight gain, and specific growth rate. The highest superoxide dismutase (SOD) and catalase (CAT) gene expression levels were seen in fish on the 0.5 g/kg CS-supplemented diet, and CS-fed groups displayed the greatest expression of the immune-related gene interleukin-1 beta (IL-1β) relative to the control. Collectively, these findings indicate that Cyanus depressus and Schizochytrium sp. extracts, especially at a dosage of 0.5 g/kg, have promising potential as dietary supplements for enhancing the physiological health and growth of carp.

Keywords

Cyanus depressus, Extract, Feed supplement, Gene expression, Schizochytrium sp.

Ethical Statement

This experiment was approved by the Van Yüzüncü Yıl University Local Ethics Committee for Animal Experiments (protocol no: 2023/13-31) and conducted in accordance with established ethical guidelines.

Supporting Institution

Van Yüzüncü Yıl University, Scientific Research Projects Department

Project Number

FHD-2024-10941

Thanks

This research was supported by the Van Yüzüncü Yıl University, Scientific Research Projects Department (FHD-2024-10941). The author thanks the Van Yuzuncu Yıl University Scientific Research Projects Department for their support.

Cyanus depressus ve Schizochytrium sp. Ekstraktlarının Sazan Balığında (Cyprinus carpio) Büyüme Endeksleri, Antioksidan ve Bağışıklık İlgili Genlerin İfade Düzeyleri Üzerindeki Sinerjik Etkileri

Abstract

Bu çalışmanın amacı Cyanus depressus ve Schizochytrium sp. özütlerinin sazan balığında büyüme performansı ile antioksidan ve bağışıklıkla ilgili genlerin ifade düzeyleri üzerindeki sinerjik etkilerini değerlendirmektir. Ortalama ağırlığı 3,71 ± 0,16 g olan balıklar 60 günlük bir süre boyunca 0 (Kontrol), 0,5 (CS05), ve 1 (CS1) g/kg seviyelerinde ekstrakt karışımı (CS) içeren diyetler alan üç besleme grubuna ayrıldı. Sonuçlar, 0,5 ve 1 g/kg CS ekstresi ile desteklenen gruplarda son vücut ağırlığında, kilo alımında ve özgül büyüme hızında önemli iyileşmeler olduğunu ortaya koydu. Süperoksit dismutaz (SOD), ve katalaz (CAT) gen ekspresyon düzeylerindeki en önemli artış 0,5 g/kg CS özütü ile beslenen balıklarda gözlendi. Tedaviler arasında bağışıklık ile ilgili interlökin-1 beta (IL-1β)'nın en yüksek ekspresyon düzeyi, kontrol grubuna göre CS özütü ile zenginleştirilmiş diyetle beslenen gruplarda bulundu. Sonuç olarak çalışmamız, Cyanus depressus ve Schizochytrium sp. özlerinin özellikle 0,5 g/kg dozunda diyet takviyesi olarak birleştirilmesi sazan balığının çeşitli fizyolojik ve sağlık yönlerini iyileştirmede önemli bir potansiyele sahip olduğunu göstermektedir.

Keywords

Cyanus depressus, Ekstrakt, Yem takviyesi, Gen ifadesi, Schizochytrium sp.

Ethical Statement

This experiment was approved by the Van Yüzüncü Yıl University Local Ethics Committee for Animal Experiments (protocol no: 2023/13-31) and conducted in accordance with established ethical guidelines.

Supporting Institution

Van Yüzüncü Yıl University, Scientific Research Projects Department

Project Number

FHD-2024-10941

Thanks

This research was supported by the Van Yüzüncü Yıl University, Scientific Research Projects Department (FHD-2024-10941). The author thanks the Van Yuzuncu Yıl University Scientific Research Projects Department for their support.

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