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Propolis Katkılı Sporcu Gıdası ve İçeceklerinin İz Elementlerinin Belirlenmesi

Year 2025, Volume: 12 Issue: 1, 157 - 164

Abstract

Bu çalışmada sporcu beslenmesinde önemli bir yere sahip sporcu gıdası olarak isimlendirilen protein tozu (Sg), milkshake (M) ve enerji içeceği (E) örneklerinin çok değerli bir arı ürünü olan superkritik akışkan propolis ekstraktı ile zenginleştirilerek yeni fonksiyonel gıdalar geliştirilmesi (Sgp, Mp, Ep), ve bu gıdaların iz element içeriklerinin belirlenmesi amaçlanmıştır. ICP-MS cihazı kullanılarak sade ve propolis ilaveli ürünler ile propolis ekstraktının element (ağır metal) içerikleri tespit edilmiştir. Yapılan çalışmada superkritik akışkan propolis ekstraktının element (ağır metal) ortalama içerik değerleri ppm cinsinden sırasıyla; Pb: 0,6933, Ni: 3,1100, Fe: 786,2433, Cu; 1,4733, Cd: TE olarak tespit edilmiştir. Ayrıca sade ve superkritik akışkan propolis ekstraktının ilave edildiği ürünlerin değerleri her bir element için ayrı ayrı değerlendirilmiş ve önemli farklılıklar görülmüştür. İncelenen ürünlerin ortalama element (ağır metal) içerik değerleri ppm cinsinden sırasıyla; Pb için; Sg: 0,117, Sgp: 0,226, M: 0,103, Mp: 0,127, E: 0,038, Ep: 0,103, Ni için; Sg: 6,35178, Sgp: 8,14263, M: 1,560, Mp: 2,137, E: 0,056, Ep: 0,079, Fe için Sg: 0,020, Sgp: 0,028, M: 31,424, Mp: 33,615, E: 1,122, Ep: 1,347, Cu için; Sg: 5,387, Sgp: 7,132, M: 3,940, Mp: 5,147, E: 0,074, Ep; 0,081, olarak tespit edilmiştir ve tüm örneklerde Cd tespit edilmemiştir. İz element değerleri her bir element için superkritik akışkan propolis ekstraktı ve ürünlere göre değerlendirilmiş ve önemli farklılıklar görülmüştür. Ayrıca tespit edilen tüm element içeriği konsantrasyonları(ppm) karşılaştırıldığında Sgp’de yüksek konsantrasyon gözlenmiştir.

References

  • Agilent Technologies. (2023). Application note: Multielement analysis of protein powders using ICP-MS. Agilent Technologies. Retrieved from https://www.agilent.com/chem/icp-ms
  • Agilent Technologies. (2024). ICP-MS: Advanced Techniques for Food and Environmental Analysis.
  • Al-Fartusie, F. S., & Mohssan, S. N. (2017). Essential trace elements and their vital roles in human body. Indian J Adv Chem Sci, 5(3), 127-136.
  • Analytik Jena. (2018). Characterization of Powdered Milk by ICP-MS. Analytik Jena.
  • Anonim (1982) World Health Organization, WHO, “Toxicological Evaluation of Certain Food Additives”, Joint FAO / WHO Expert Committee of Food Additives. WHO Food Additives Series, number 17.
  • Anonim, (1989) FAO-WHO Food Standart Programme. Codex Standart for Sugar (Honey). CAC/vol. III. Ed. 1, supplement 2.
  • Bakkaloğlu, Z., & Arıcı, M. (2019). Farklı çözücülerle propolis ekstraksiyonunun toplam fenolik içeriği, antioksidan kapasite ve antimikrobiyal aktivite üzerine etkileri. Akademik Gıda, 17(4), 538-545.
  • Bayram, N. E. (2020). A study on free-radical scavenging activity, individual phenolic compounds and element concentration of propolis. Uludağ Arıcılık Dergisi, 20(2), 145-156.
  • Biorxiv. (2020). ICP-MS analysis of metal and metalloid concentrations of common microbiological growth media reveals presence of heavy metals. bioRxiv.
  • Budianta, D., Napoleon, A., & Habi, M. L. (2022, March). Save our soil from heavy metals (Pb and Cd) accumulation for rice growth. In IOP Conference Series: Earth and Environmental Science (Vol. 1005, No. 1, p. 012001). IOP Publishing.
  • Coşkun, P., & İnci, H. (2020). Antibacterial, antiviral, antioxidant activity and chemical content of propolis. ISPEC Tarım Bilimleri Dergisi, 4(4), 1053-1070.
  • Döner, Ö., & İnci, H. (2021). Bingöl ilinin farklı bölgelerinden elde edilen propolislerin protein oranı ve kül miktarı açısından karşılaştırılması. ISPEC Journal of Agricultural Sciences, 5(2), 372-380.
  • Golubkina, N. A., Sheshnitsan, S. S., Kapitalchuk, M. V., & Erdenotsogt, E. (2016). Variations of chemical element composition of bee and beekeeping products in different taxons of the biosphere. Ecological indicators, 66, 452-457.
  • Idrus, N. F. M., Yian, L. N., Idham, Z., Aris, N. A., Putra, N. R., Aziz, A. H. A., & Yunus, M. A. C. (2018). Mini review: Application of supercritical carbon dioxide in extraction of propolis extract. J. Malays. J. Fundam. Appl. Sci, 14, 387-396.
  • İlkaya, M., & İnci, H. (2023). Bingöl İlinin Farklı Bölgelerinden Toplanmış Apilarnilin İz Element (Ağır Metal) İçeriğinin Belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 10(3), 715-725.
  • İzol, E., & Turhan, M. (2024). In-Depth Phytochemical Profile by LC-MS/MS, Mineral Content by ICP-MS, and In-Vitro Antioxidant, Antidiabetic, Antiepilepsy, Anticholinergic, and Antiglaucoma Properties of Bitlis Propolis. Life, 14(11), 1389.
  • Kafouris, D., Christoforou, E., Stefani, D., Sarandi, A., Stavroulakis, G., Christou, E., & Yiannopoulos, S. (2024). Lead, cadmium and mercury determination and human health risk assessement in foods from Cyprus. Journal of Food Composition and Analysis, 128, 106007.
  • Kebede, I. A., Gebremeskel, H. F., & Ahmed, A. D. (2024). Bee products and their processing: a review. Pharm Pharmacol Int J, 12(1), 5-12.
  • Khaneghah, A. M., Fakhri, Y., Nematollahi, A., & Pirhadi, M. (2020). Potentially toxic elements (PTEs) in cereal-based foods: a systematic review and meta-analysis. Trends in Food Science & Technology, 96, 30-44.
  • Matuszewska, E., Klupczynska, A., Maciołek, K., Kokot, Z. J., & Matysiak, J. (2021). Multielemental analysis of bee pollen, propolis, and royal jelly collected in west-central Poland. Molecules, 26(9), 2415.
  • Mundi, A. A., Ibrahim, U., & Mustapha, I. M. (2019). Contamination and Pollution Risk Assessment of Heavy Metals in Rice Samples (Oryza sativa) from Nasarawa West, Nigeria. Asian Journal of Advanced Research and Reports, 3(4), 1-8.
  • Murashova, E. A., Tunikov, G. M., & Nefedova, S. A. (2020). The main factors determining the accumulation of poisonous elements by bees and honey products. International Journal of Transactions in Engineering, Management and Applied Sciences and Technologies, 3, 1-14.
  • Pastukhova, M. A., Galuts, O. A., Dashkevich, M. M., & Mikhalchuk, S. N. (2016). Peculiarities of accumulation of heavy metals in the system soil-plant-bee-beekeeping products. Natural resource management, 30, 70-75.
  • Roman, A., Madras-Majewska, B., & Popiela-Pleban, E. (2011). Comparative study of selected toxic elements in propolis and honey. J. Apic. Sci, 55(2), 97-106.
  • Ring, J. M., Kelliher, A., & McCormick, K. L. (2021). Analysis of trace elements in protein powders from the Irish market using ICP-MS. Food Chemistry, 345, 128-136. https://doi.org/10.1016/j.foodchem.2020.128136
  • Soós, Á., Bódi, É., Várallyay, S., Molnár, S., & Kovács, B. (2021). Microwave-assisted sample preparation of Hungarian raw propolis in quartz vessels and element analysis by ICP-OES and ICP-MS for geographical identification. Talanta, 233, 122613.
  • Taulavuori, K., Julkunen-Tiitto, R., Hyöky, V., & Taulavuori, E. (2013). Blue mood for superfood. Natural Product Communications, 8(6), 1934578X1300800627.
  • Vakhonina, E. A., Lapynina, E. P., & Lizunova, A. S. (2021, November). Study of toxic elements in propolis. In IOP Conference Series: Earth and Environmental Science (Vol. 845, No. 1, p. 012122). IOP Publishing.

Determination of Trace Elements in Propolis Added Sports Food and Beverages

Year 2025, Volume: 12 Issue: 1, 157 - 164

Abstract

In this study, it was aimed to develop new functional foods (Sgp, Mp, Ep) by enriching protein powder (Sg), milkshake (M) and energy drink (E) samples, which are named as sports foods that have an important place in sports nutrition, with supercritical fluid propolis extract, a very valuable bee product, and to determine the trace element contents of these foods. In this study, it was aimed to develop new functional foods (Sgp, Mp, Ep) by enriching protein powder (Sg), milkshake (M) and energy drink (E) samples, which have an important place in sports nutrition, with supercritical fluid propolis extract, a very valuable bee product, and to determine the trace element contents of these foods. The element (heavy metal) contents of plain and propolis added products and propolis extract were determined using ICP-MS device. In the study, the average element (heavy metal) content values of the supercritical fluid propolis extract in ppm were as follows; Pb: 0,6933, Ni: 3,1100, Fe: 786,2433, Cu: 1,4733, Cd: TE. In addition, the values of the products to which plain and supercritical fluid propolis extract was added were evaluated separately for each element and significant differences were observed. The average element (heavy metal) content values of the analyzed products in ppm are as follows; for Pb; Sg: 0,117, Sgp: 0,226, M: 0,103, Mp: 0,127, E: 0,038, Ep: 0,103, for Ni; Sg: 6,35178, Sgp: 8,14263, M: 1,560, Mp: 2,137, E: 0,056, Ep: 0,079, for Fe; Sg: 0,020, Sgp: 0,028, M: 31,424, Mp: 33,615, E: 1,122, Ep: 1,347, for Cu; Sg: 5,387, Sgp: 7,132, M: 3,940, Mp: 5,147, E: 0,074, Ep: 0,081, and Cd was not detected in all samples. Trace element values were evaluated for each element according to supercritical fluid propolis extract and products and significant differences were observed. In addition, when the concentrations (ppm) of all detected elemental contents were compared, high concentrations were observed in Sgp.

References

  • Agilent Technologies. (2023). Application note: Multielement analysis of protein powders using ICP-MS. Agilent Technologies. Retrieved from https://www.agilent.com/chem/icp-ms
  • Agilent Technologies. (2024). ICP-MS: Advanced Techniques for Food and Environmental Analysis.
  • Al-Fartusie, F. S., & Mohssan, S. N. (2017). Essential trace elements and their vital roles in human body. Indian J Adv Chem Sci, 5(3), 127-136.
  • Analytik Jena. (2018). Characterization of Powdered Milk by ICP-MS. Analytik Jena.
  • Anonim (1982) World Health Organization, WHO, “Toxicological Evaluation of Certain Food Additives”, Joint FAO / WHO Expert Committee of Food Additives. WHO Food Additives Series, number 17.
  • Anonim, (1989) FAO-WHO Food Standart Programme. Codex Standart for Sugar (Honey). CAC/vol. III. Ed. 1, supplement 2.
  • Bakkaloğlu, Z., & Arıcı, M. (2019). Farklı çözücülerle propolis ekstraksiyonunun toplam fenolik içeriği, antioksidan kapasite ve antimikrobiyal aktivite üzerine etkileri. Akademik Gıda, 17(4), 538-545.
  • Bayram, N. E. (2020). A study on free-radical scavenging activity, individual phenolic compounds and element concentration of propolis. Uludağ Arıcılık Dergisi, 20(2), 145-156.
  • Biorxiv. (2020). ICP-MS analysis of metal and metalloid concentrations of common microbiological growth media reveals presence of heavy metals. bioRxiv.
  • Budianta, D., Napoleon, A., & Habi, M. L. (2022, March). Save our soil from heavy metals (Pb and Cd) accumulation for rice growth. In IOP Conference Series: Earth and Environmental Science (Vol. 1005, No. 1, p. 012001). IOP Publishing.
  • Coşkun, P., & İnci, H. (2020). Antibacterial, antiviral, antioxidant activity and chemical content of propolis. ISPEC Tarım Bilimleri Dergisi, 4(4), 1053-1070.
  • Döner, Ö., & İnci, H. (2021). Bingöl ilinin farklı bölgelerinden elde edilen propolislerin protein oranı ve kül miktarı açısından karşılaştırılması. ISPEC Journal of Agricultural Sciences, 5(2), 372-380.
  • Golubkina, N. A., Sheshnitsan, S. S., Kapitalchuk, M. V., & Erdenotsogt, E. (2016). Variations of chemical element composition of bee and beekeeping products in different taxons of the biosphere. Ecological indicators, 66, 452-457.
  • Idrus, N. F. M., Yian, L. N., Idham, Z., Aris, N. A., Putra, N. R., Aziz, A. H. A., & Yunus, M. A. C. (2018). Mini review: Application of supercritical carbon dioxide in extraction of propolis extract. J. Malays. J. Fundam. Appl. Sci, 14, 387-396.
  • İlkaya, M., & İnci, H. (2023). Bingöl İlinin Farklı Bölgelerinden Toplanmış Apilarnilin İz Element (Ağır Metal) İçeriğinin Belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 10(3), 715-725.
  • İzol, E., & Turhan, M. (2024). In-Depth Phytochemical Profile by LC-MS/MS, Mineral Content by ICP-MS, and In-Vitro Antioxidant, Antidiabetic, Antiepilepsy, Anticholinergic, and Antiglaucoma Properties of Bitlis Propolis. Life, 14(11), 1389.
  • Kafouris, D., Christoforou, E., Stefani, D., Sarandi, A., Stavroulakis, G., Christou, E., & Yiannopoulos, S. (2024). Lead, cadmium and mercury determination and human health risk assessement in foods from Cyprus. Journal of Food Composition and Analysis, 128, 106007.
  • Kebede, I. A., Gebremeskel, H. F., & Ahmed, A. D. (2024). Bee products and their processing: a review. Pharm Pharmacol Int J, 12(1), 5-12.
  • Khaneghah, A. M., Fakhri, Y., Nematollahi, A., & Pirhadi, M. (2020). Potentially toxic elements (PTEs) in cereal-based foods: a systematic review and meta-analysis. Trends in Food Science & Technology, 96, 30-44.
  • Matuszewska, E., Klupczynska, A., Maciołek, K., Kokot, Z. J., & Matysiak, J. (2021). Multielemental analysis of bee pollen, propolis, and royal jelly collected in west-central Poland. Molecules, 26(9), 2415.
  • Mundi, A. A., Ibrahim, U., & Mustapha, I. M. (2019). Contamination and Pollution Risk Assessment of Heavy Metals in Rice Samples (Oryza sativa) from Nasarawa West, Nigeria. Asian Journal of Advanced Research and Reports, 3(4), 1-8.
  • Murashova, E. A., Tunikov, G. M., & Nefedova, S. A. (2020). The main factors determining the accumulation of poisonous elements by bees and honey products. International Journal of Transactions in Engineering, Management and Applied Sciences and Technologies, 3, 1-14.
  • Pastukhova, M. A., Galuts, O. A., Dashkevich, M. M., & Mikhalchuk, S. N. (2016). Peculiarities of accumulation of heavy metals in the system soil-plant-bee-beekeeping products. Natural resource management, 30, 70-75.
  • Roman, A., Madras-Majewska, B., & Popiela-Pleban, E. (2011). Comparative study of selected toxic elements in propolis and honey. J. Apic. Sci, 55(2), 97-106.
  • Ring, J. M., Kelliher, A., & McCormick, K. L. (2021). Analysis of trace elements in protein powders from the Irish market using ICP-MS. Food Chemistry, 345, 128-136. https://doi.org/10.1016/j.foodchem.2020.128136
  • Soós, Á., Bódi, É., Várallyay, S., Molnár, S., & Kovács, B. (2021). Microwave-assisted sample preparation of Hungarian raw propolis in quartz vessels and element analysis by ICP-OES and ICP-MS for geographical identification. Talanta, 233, 122613.
  • Taulavuori, K., Julkunen-Tiitto, R., Hyöky, V., & Taulavuori, E. (2013). Blue mood for superfood. Natural Product Communications, 8(6), 1934578X1300800627.
  • Vakhonina, E. A., Lapynina, E. P., & Lizunova, A. S. (2021, November). Study of toxic elements in propolis. In IOP Conference Series: Earth and Environmental Science (Vol. 845, No. 1, p. 012122). IOP Publishing.
There are 28 citations in total.

Details

Primary Language Turkish
Subjects Bee and Silkworm Breeding and Improvement
Journal Section Research Article
Authors

Pınar Coşkun 0000-0002-9170-5799

Hakan İnci 0000-0002-9791-0435

Early Pub Date January 25, 2025
Publication Date
Submission Date December 5, 2024
Acceptance Date December 19, 2024
Published in Issue Year 2025 Volume: 12 Issue: 1

Cite

APA Coşkun, P., & İnci, H. (n.d.). Propolis Katkılı Sporcu Gıdası ve İçeceklerinin İz Elementlerinin Belirlenmesi. Turkish Journal of Agricultural and Natural Sciences, 12(1), 157-164. https://doi.org/10.30910/turkjans.1596673