Impact of Fe-EDTA, EDTA, and Zn Applications on Fe and Zn Levels and Biomass Production in Bread Wheat

Year 2025, Volume: 12 Issue: 4, 1109 - 1119, 17.10.2025

Faruk Özkutlu , Kürşat Korkmaz

https://doi.org/10.30910/turkjans.1760301

Abstract

Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), are a persistent constraint to crop productivity and nutritional quality in calcareous soils with high pH. This study investigated the effects of EDTA (ethylene dinitrilo tetraacetic acid disodium salt dihydrate), Fe-EDTA in combination with different Zn fertilization rates on dry matter yield and tissue Fe and Zn status of bread wheat (Triticum aestivum L., cv. BDME-10). A greenhouse experiment was conducted in a randomized complete block design with four replications. Treatments included Fe-EDTA applied at 0, 2, and 10 mg Fe kg-1, unchelated EDTA at 0, 13, and 65 mg kg-1, together with Zn sulfate at 0, 0.05, and 5 mg Zn kg-1. Both Fe and Zn fertilization enhanced biomass production and Fe and Zn concentrations. The greatest yield improvement, corresponding to a 43% increase compared with control, was obtained with 10 mg Fe kg-1 as Fe-EDTA in combination with 5 mg Zn kg-1. Zinc application also markedly increased Zn uptake, with Zn uptake rising from 1.61 µg plant-1 in the control plants to 37.26 µg plant-1 at the highest Zn dose. However, this increase in Zn dosage led to reductions in Fe concentration and uptake of up to 48%. In Fe-EDTA treatments, Fe uptake reached 76.94 µg plant-1 at moderate Zn supply but decreased to 56.62 µg plant-1 under excess Zn. EDTA, however, also increased both Fe and Zn uptake, yet its effectiveness remained consistently lower than that of Fe-EDTA under comparable conditions. These results demonstrate that Fe-EDTA is a more efficient, supporting higher Fe acquisition and Zn accumulation while reducing the severity of Zn–Fe antagonism. A balanced combination of Fe-EDTA and Zn supply therefore represents a promising approach to improve both yield and Fe and Zn content of wheat in calcareous soils.

Keywords

Micronutrient uptake , Chelates , Chelated iron , Fe uptake , Zn uptake

Ethical Statement

Ethics committee approval was not required for this study, as it did not involve any human participants or animal experimentation.

Project Number

No

References

• Ali, I., Khan, A., Ali, A., Ullah, Z., Dai, D.-Q., Khan, N., Khan, A., Al-Tawaha, A. R., & Sher, H. (2022). Iron and zinc micronutrients and soil inoculation of Trichoderma harzianum enhance wheat grain quality and yield. Frontiers in Plant Science, 13, 960948.
• Alloway, B. J. (2009). Soil factors associated with zinc deficiency in crops and humans. Environmental geochemistry and health, 31(5), 537-548.
• Aydemir, Ö. E., Akgün, M., Erdem, H., Korkmaz, K., & Özkutlu, F. (2023). The effect of different lime forms on cadmium uptake of durum wheat varieties. Turkish Journal of Agriculture-Food Science and Technology, 11(8), 1365-1371.
• Bouis, H. E., Hotz, C., McClafferty, B., Meenakshi, J. V., & Pfeiffer, W. H. (2011). Biofortification: a new tool to reduce micronutrient malnutrition. Food and Nutrition Bulletin, 32(1 suppl), 31S-40S.
• Bouyoucos, G. J. (1951). A Recalibration of the hydrometer for making mechanical analysis of soils. Agronomy Journal, 43, 434-438.
• Çağlar KÖ. 1949. Toprak bilgisi. Ankara Üniversitesi Ziraat Fak. Yayınları Sayı:10.
• Diaz, S., Polania, J., Ariza-Suarez, D., Cajiao, C., Grajales, M., Raatz, B., & Beebe, S. E. (2022). Genetic correlation between Fe and Zn biofortification and yield components in a common bean (Phaseolus vulgaris L.). Frontiers in Plant Science, 12, 739033.
• Hansch, R., & Mendel, R. R. (2009). Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Current Opinion in Plant Biology, 12(3), 259–266.
• Hao, B., Ma, J., Jiang, L., Wang, X., Bai, Y., Zhou, C., ... & Wang, Z. (2021). Effects of foliar application of micronutrients on concentration and bioavailability of zinc and iron in wheat landraces and cultivars. Scientific Reports, 11(1), 22782.
• Havlin, J. L., Tisdale, S. L., Nelson, W. L., & Beaton, J. D. (2013). Soil fertility and fertilizers (8th ed.). Pearson.
• Heidarian, A. R., Kord, H., Mostafavi, K., Lak, A. P., & Mashhadi, F. A. (2011). Investigating Fe and Zn foliar application on yield and its components of soybean (Glycine max (L) Merr.) at different growth stages. Journal of Agricultural Biotechnology and Sustainable Development, 3(9), 189.
• Hotz, C., & Brown, K. H. (2004). Assessment of the risk of zinc deficiency in populations and options for its control. Food and Nutrition Bulletin, 25(1 Suppl 1), S93–S203.
• Jackson, M. L. (1959). Soil Chemical Analysis, Englewood Cliffs, New Jersey.
• Kabata-Pendias, A., & Pendias, H. (1999). Biogeochemistry of Trace Elements. Polish Scientific Publishing Company.
• Khan, H. R., McDonald, G. K., & Rengal, Z. (2003). Zn fertilization improves water use efficiency grain yield and seed Zn content in Chickpea. Plant and Soil, 249, 389–400.
• Kobayashi, T., & Nishizawa, N. K. (2012). Iron uptake, translocation, and regulation in higher plants. Annual Review of Plant Biology, 63, 131–152.
• Kobraee, S., & Shamsi, K. (2011). Effects of Zn, Fe and Mn on soybean production. Ecology, Environment and Conservation, 17(2), 191-96.
• Korkmaz, K., Kirli, A., Akgun, M., & Dede, O. (2018). Effects of different levels of foliar zinc and application time on total phenolic content and antioxidant activity of potato. Fresenius Environ. Bull, 27, 4192-4197.
• Korkmaz, K., Akgün, M., Özcan, M. M., Özkutlu, F., & Kara, Ş. M. (2021). Interaction effects of phosphorus (P) and zinc (Zn) on dry matter, concentration and uptake of P and Zn in chia. Journal of Plant Nutrition, 44(5), 755-764.
• Korkmaz, K., Kılıç, R., Akgün, M., & Kara, Ş. M. (2024). Effects of combining phosphorus (P) and zinc (Zn) fertilization on P-Zn distribution and yield in safflower. Journal of Plant Nutrition, 47(10), 1585-1595.
• Li, M., Wang, S., Tian, X., Li, S., Chen, Y., Jia, Z., & Liu, K. (2016). Zinc and iron concentrations in grain milling fractions through combined foliar applications of Zn and macronutrients. Field Crops Research, 187, 135–141.
• Lindsay, W. L., & Norvell, W. A. (1978). Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal, *42*(3), 421–428.
• Lucena, J. J. (2006). Synthetic iron chelates to correct iron deficiency in plants. In L. L. Barton & J. Abadía (Eds.), Iron nutrition in plants and rhizospheric microorganisms (pp. 103–128). Springer.
• Mannan, M. A., Tithi, M. A., Islam, M. R., Mamun, M. A. A., Mia, S., Rahman, M. Z., Awad, M. F., ElSayed, A. I., Mansour, E., & Hossain, M. S. (2022). Soil and foliar applications of zinc sulfate and iron sulfate alleviate the destructive impacts of drought stress in wheat. Cereal Research Communications, 50, 1279–1289.
• Marcos-Barbero, E. L., Perez, P., Martinez-Carrasco, R., Arellano, J. B., & Morcuende, R. (2021). Genotypic variability on grain yield and grain nutritional quality characteristics of wheat grown under elevated CO2 and high temperature. Plants, 10(5), 1043.
• Müller, O., & Krawinkel, M. (2005). Malnutrition and health in developing countries. CMAJ: Canadian Medical Association Journal, 173(3), 279–286.
• Mutari, B., Sibiya, J., Gasura, E., Kondwakwenda, A., Matova, P. M., & Chirwa, R. (2022). Genotype x environment interaction and stability analyses of grain yield and micronutrient (Fe and Zn) concentrations in navy bean (Phaseolus vulgaris L.) genotypes under varied production environments. Field Crops Research, 286, 108607.
• Nadim, M. A., Awan, I. U., Baloch, M. S., Khan, E. A., Naveed, K., & Khan, M. A. (2012). Response of wheat (Triticum aestivum L.) to different micronutrients and their application methods. The Journal of Animal & Plant Sciences, 22(1), 113–119.
• Niyigaba, E., Twizerimana, A., Mugenzi, I., Ngnadong, W. A., Ye, Y. P., Wu, B. M., & Hai, J. B. (2019). Winter Wheat Grain Quality, Zinc and Iron Concentration Affected by a Combined Foliar Spray of Zinc and Iron Fertilizers. Agronomy, 9(5), 250.
• Olsen, S. N., Cole, C. V., Watanabe, F. S., Dean, L. A. (1954). Estimation of available phosphorus in soils by Extraction with Sodiumbicarbonate. USDA, Circ. 939 p.
• Pirzad, A., & Barin, M. (2018). Iron and zinc interaction on leaf nutrients and the essential oil of Pimpinella anisum L. Iranian Journal of Plant Physiology, 8(4), 2507–2515.
• Pahlavan‐Rad, M. R., & Pessarakli, M. (2009). Response of wheat plants to zinc, iron, and manganese applications and uptake and concentration of zinc, iron, and manganese in wheat grains. Communications in soil science and plant analysis, 40(7-8), 1322-1332.
• Ram, S., Malik, V. K., Gupta, V., Narwal, S., Sirohi, M., Ankush, Pandey, V., Gupta, O. P., Misra, A. K., & Singh, G. (2024). Impact of foliar application of iron and zinc fertilizers on grain iron, zinc, and protein contents in bread wheat (Triticum aestivum L.). Frontiers in Nutrition, 11, 1378937.
• Roberts, C., Steer, T., Maplethorpe, N., & Knight, B. (2018). National Diet and Nutrition Survey Results from Years 7 and 8 (Combined) of the Rolling Programme (2014/2015–2015/2016). Public Health England and Food Standards Agency.
• Şahin, C. B., & İşler, N. (2021). Foliar Applied Zinc and Iron Effects on Yield and Yield Components of Soybean: Determination by PCA Analysis. Communications in Soil Science and Plant Analysis, 52(3), 212–221.
• San, Z. F. (2006). Trace elements and human health. Studies of Trace Elements and Health, 23(3), 66–67.
• Sher, A., Sarwar, B., Sattar, A., Ijaz, M., Ul-Allah, S., Hayat, M. T., Manaf, A., Qayyum, A., Zaheer, A., Iqbal, J., El Askary, A., Gharib, A.F., Ismail, K.A., & Elesawy, B. H. (2022). Exogenous application of zinc sulphate at heading stage of wheat improves the yield and grain zinc biofortification. Agronomy, 12(3), 734.
• Shi, R., Zhang, Y., Chen, X., Sun, Q., Zhang, F., Römheld, V., & Zou, C. (2010). Iron and Zinc Concentrations in Grain and Flour of Winter Wheat As Affected by Foliar Application. Journal of Agricultural and Food Chemistry, 58(23), 12268–12274.
• Stein, A. J. (2010). Global impacts of human mineral malnutrition. Plant and Soil, 335(1), 133–154. Sultana, N., Ikeda, T., & Kashem, M. A. (2001). Effect of foliar spray of nutrient solutions on photosynthesis dry matter accumulation and yield in seawater-stressed rice. Environmental and Experimental Botany, 46(2), 129–140.
• U.S. Salinity Laboratory Staff. (1954). USDA Handbook no. 60. Diagnosis and improvement of saline and alkali soils. Washington, D.C. U.S. Government Printing Office.
• Vaghar, M. S., Sayfzadeh, S., Zakerin, H. R., Kobraee, S., & Valadabadi, S. A. (2020). Foliar application of iron, zinc, and manganese nano-chelates improves physiological indicators and soybean yield under water deficit stress. Journal of Plant Nutrition, 43(18), 2740–2756.
• Velu, G., Crespo Herrera, L., Guzman, C., Huerta, J., Payne, T., & Singh, R. P. (2019). Assessing Genetic Diversity to Breed Competitive Biofortified Wheat With Enhanced Grain Zn and Fe Concentrations. Frontiers in Plant Science, 9, 1971.
• Walkley, A., Black, I. A. (1934). An examination of degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science, 37, 29-37.
• Wani, S. H., Gaikwad, K., Razzaq, A., Samantara, K., Kumar, M., & Govindan, V. (2022). Improving zinc and iron biofortification in wheat through genomics approaches. Molecular Biology Reports, 49(8), 8007-8023.