FARKLI DOZLARDA PEG 6000 UYGULAMALARININ SAFRANDA İN VİTRO REJENERASYONA ETKİLERİ

Year 2021, Volume: 5 Issue: 2, 408 - 417, 31.10.2021

Başar Sevindik

Abstract

Safran olarak bilinen Crocus sativus L., eski çağlardan beri kuru stigmaları baharat olarak kullanılan önemli bir bitkidir. Son zamanlarda abiyotik stres bitkisel üretimde karşılaşılan en önemli sorunlardan birisidir. Kuraklık stresi, bitkinin gelişimini farklı seviyelerde sınırlayan en önemli abiyotik stres olarak bilinmektedir. Kuraklık stresine dayanıklı bitkilerin seçilmesinde in vitro seleksiyon yöntemi oldukça önemli bir tekniktir. Polyetilen glikol (PEG), toksik olmayan ve bitkiye tutunmayan bir kimyasaldır ve genelde bitkilerin kuraklığa gösterdiği tepkileri ölçmek adına yapılan kuraklık stresi çalışmalarında simülatör olarak kullanılmaktadır. Bu çalışmada, Crocus sativus L., bitkisinin kormları 30 g L-1 sakkaroz, 2 mg L-1 2,4-D + 1 mg L-1 2iP, 7 g L-1 agar ve 0, 5%, 10%, 20% PEG 6000 ile kombine edilmiş MS besi yerinde kültüre alınmıştır. En etkili in vitro rejenerasyonun PEG içermeyen MS besi yerinde olduğu belirlenmiştir. Rejenere olan eksplantlar, sürgün oluşumu elde etmek için bitki büyüme düzenleyicisi içermeyen MS besi yerine transfer edilmiştir. Meydana gelen embryojenik yapıların farklı embriyo aşamalarında tutuklu kaldığı ve sürgün oluşmadığı gözlenmemiştir. Aynı besi yerinde organogenesis sonucu oluşan korm benzeri yapılarda da gelişim sınırlı kalmış ve bitkiye dönüşüm gerçekleşmemiştir.

Keywords

Crocus sativus L., doku kültürü, MS, kuraklık, stres

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