Investigation of Ghrelin, Obestatin, Leptin Expressions in Obesity Patients Who Have Had Sleeve Gastrectomy

Abstract

Investigation of Ghrelin, Obestatin, Leptin Expressions in Obesity Patients Who Have Had Sleeve Gastrectomy ABSTRACT Background: Obesity has become an important public health problem that affects the whole society because it causes many diseases. In this study, it was aimed to investigate the expression rate of ghrelin, leptin and obestatin hormones released from the stomach and the relationship between obesity and diabetes. Materials and Methods: In the study, gastric tissues and clinical data of patients with obesity who underwent sleeve gastrectomy and 126 patients who underwent gastric biopsy for non-obesity reasons were examined. Hematoxylin-eosin staining method was used for the histopathological evaluation of the cases. To determine the expression rate of ghrelin, leptin and obestatin hormones in gastric tissue, immunohistochemistry staining method was applied. In addition, clinical findings in both groups were examined. The data obtained from the study were evaluated statistically. Results: Ghrelin and obestatin levels were lower in obese individuals, while leptin levels were higher. In addition, it was observed that the expression of ghrelin and obestatin decreased with increasing age. It was shown that ghrelin expression is higher in obese women, leptin expression is higher in non-obese men, and obestatin expression is lower in obese men. In addition, in our study, while ghrelin levels were lower in diabetic obese individuals, leptin and obestatin levels were higher. Conclusions: As a result, it was concluded that the expression levels of ghrelin, leptin and obestatin hormones may be effective in the formation of obesity and obesity-related diabetes. Keywords: Gastric sleeve surgery, stomach hormones, immünohistochemistry

Keywords

• gastric sleeve surgery
• stomach hormones
• immünohistochemistry

Project Number

TDK-2018-8198

Sleeve Gastrektomi Uygulanmış Obezite Hastalarında Ghrelin, Obestatin, Leptin Ekspreyonları’nın Araştırılması

Abstract

Sleeve Gastrektomi Uygulanmış Obezite Hastalarında Ghrelin, Obestatin, Leptin Ekspreyonları’nın Araştırılması ÖZET Amaç: Obezite, birçok hastalığın oluşmasına neden olduğu için tüm toplumu etkileyen önemli bir halk sağlığı problemi haline gelmiştir. Bu çalışmada, mideden salınan ghrelin, leptin ve obestatin hormonlarının ekspresyon oranı ile obezitenin diyabet ile olan ilişkisinin araştırılması amaçlanmıştır. Araçlar ve Yöntem: Çalışmada, Sleeve gastrektomi ameliyatı olmuş obezite hastaları ile obezite dışı nedenlerle mide biyopsisi yapılmış toplam 126 hastanın mide dokuları ve hastaların klinik verileri incelendi. Vakaların histopatolojik değerlendirmesi için hematoksilen eozin boyama yöntemi kullanıldı. Mide dokusundaki ghrelin, leptin ve obestatin hormonlarının ekspresyon oranını belirlemek için immünohistokimya boyama yöntemi uygulandı. Ayrıca her iki gruptaki klinik bulgular incelendi. Çalışmadan elde edilen veriler istatistiksel olarak değerlendirildi. Bulgular: Obez bireylerde ghrelin ve obestatin seviyesi daha düşük iken leptin seviyesi yüksek bulundu. Ayrıca, ghrelin ve obestatin ekspresyonunun yaş ilerledikçe azaldığı gözlendi. Obez kadınlarda ghrelin ekspresyonunun daha yüksek olduğu, obez olmayan erkeklerde leptin ekspresyonunun daha yüksek olduğu ve obez erkeklerde obestatin ekspresyonunun daha düşük olduğu gösterildi. Ayrıca, çalışmamızda diyabetik obez bireylerde ghrelin seviyesi daha düşük iken leptin ve obestatin seviyelerinin daha yüksek olduğu gözlendi. Sonuç: Ghrelin, leptin ve obestatin hormonlarının ekspresyon düzeylerinin obezite ve obeziteye bağlı diyabet oluşumunda etkili olabileceği sonucuna varıldı. Anahtar Kelimeler: Tüp mide ameliyatı, mide hormonları, immünohistokimya

Keywords

• mide hormonları
• immünohistokimya
• tüp mide ameliyatı

Supporting Institution

Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir

Project Number

TDK-2018-8198

Thanks

Bu çalışma TDK-2018-8198 numaralı proje ile Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Katkılarından dolayı Dr. Öğretim Üyesi Münevver Baran’a teşekkür ederim.

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