Investigation of Common Bean (Phaseolus vulgaris L.) Varieties in Türkiye for Resistance Sources to Angular Leaf Spot Disease (Pseudocercospora griseola)

Abstract

This research aimed to investigate resistance sources to angular leaf spot disease (Pseudocercospora griseola) on some registered bean (Phaseolus vulgaris L.) varieties widely grown in Türkiye using SCAR markers (SN02, SH13, and g2303). The studies were carried out in the laboratories of Ankara University and Bolu Abant İzzet Baysal University Plant Protection Department in 2020-2021. The findings revealed that the most common gene among the varieties was Phg-2, followed by Phg-ON and Phg-1 genes, respectively. When bean cultivars were examined in terms of different gene combinations, it was determined that 18 cultivars had 1 gene, 41 cultivars had 2 genes, and 5 cultivars (Yalova-17, Boncuk, Aslan, Helda, Akman-98) had all three resistance genes. It is predicted that the determined resistance genes and bean varieties with combined resistance may contribute to the breeding studies to be carried out on angular leaf spot.

Keywords

• Resistance Gene
• Common bean
• Angular leaf spot
• Molecular marker

Project Number

120O576

Türkiye’deki Fasulye (Phaseolus vulgaris L.) Çeşitlerinin Köşeli Yaprak Lekesi Hastalığı (Pseudocercospora griseola)’na Karşı Dayanıklılık Kaynaklarının Araştırılması

Abstract

Bu çalışmada, Türkiye'de yaygın olarak yetiştiriciliği yapılan bazı tescilli fasulye (Phaseolus vulgaris L.) çeşitlerinin köşeli yaprak lekesi hastalığı (Pseudocercospora griseola)’na karşı dayanıklılık kaynaklarının SCAR markörler (SN02, SH13 ve g2303) aracılığıyla incelenmesi amaçlanmıştır. Çalışmalar, 2020-2021 yıllarında, Ankara Üniversitesi ve Bolu Abant İzzet Baysal Üniversitesi Bitki Koruma Bölüm laboratuvarlarında gerçekleştirilmiştir. Çalışma sonucunda elde edilen bulgular, çeşitler arasında en yaygın genin Phg-2 olduğunu, bunu sırasıyla Phg-ON ve Phg-1 genlerinin izlediğini göstermiştir. Farklı gen kombinasyonları bakımından fasulye çeşitleri incelendiğinde; 18 çeşidin 1 gen, 41 çeşidin 2 gen, 5 çeşidin (Yalova-17, Boncuk, Aslan, Helda, Akman-98) ise her üç dayanıklılık genine sahip olduğu tespit edilmiştir. Tespit edilen dayanıklılık genlerinin ve kombine dayanıklılığa sahip fasulye çeşitlerinin köşeli yaprak lekesi ile ilgili yürütülecek ıslah çalışmalarına katkı sağlayabileceği öngörülmektedir.

Keywords

• Dayanıklılık geni
• Fasulye
• Köşeli yaprak lekesi
• Moleküler markör

Supporting Institution

TÜBİTAK

Project Number

120O576

References

1. Abadio, A.K.R., Lima, S.S., Santana, M.F., Salomão, T.M.F., Sartorato, A., Mizubuti, E.S.G., Araújo, E.F., de Queiroz, M.V., 2012. Genetic diversity analysis of isolates of the fungal bean pathogen Pseudocercospora griseola from central and southern Brazil. Genetic and Molecular Research, 11(2): 1272-1279.
2. Aggarwal, V.D., Pastor-Corrales, M.A., Chirwa, R.M., Buruchara, R.A., 2004. Andean beans (Phaseolus vulgaris L.) with resistance to the angular leaf spot pathogen (Phaeoisariopsis griseola) in southern and eastern Africa. Euphytica, 136(2): 201-210.
3. Anonim, 2021. Bitkisel Üretim İstatistikleri. Türkiye İstatistik Kurumu, (http://www.tuik.gov.tr), (Erişim tarihi: 20.02.2021).
4. Anonymous, 1996. Fasulye Dayanıklılık Kaynakları. International Center for Tropical Agriculture (CIAT), (https://www.ciat.com/), (Erişim tarihi: 10.01.2022).
5. Anonymous, 2012. DNA Ekstraksiyon Yöntemi. Diversity Arrays Technology, (http://www. diversityarrays.com), (Erişim tarihi: 20.03.2021).
6. Anonymous, 2021. Crops and Livestock Products. Food and Agriculture Organization of the United Nations, (https://www.fao.org/faostat/en/#data/QCL), (Erişim tarihi: 20.04.2021).
7. Busogoro, J.P., Jijakli, M.H., Lepoivre, P., 1999a. Identification of a novel source of resistance to angular leaf spot disease of common bean within the secondary gene pool. Plant Breeding, 118(5): 417-423.
8. Busogoro, J.P., Jijakli, M.H., Lepoivre, P., 1999b. Virulence variation and RAPD polymorphism in African isolates of Phaeoisariospis griseola (Sacc.) Ferr., the causal agent of angular leaf spot of common bean. European Journal of Plant Pathology, 105(6): 559-569.

9. Caixeta, E.T., Borém, A., Alzate-Marin, A.L., Fagundes, S.D.A., Silva, M.G.D.M., de Barros, E.G., Moreira, M.A., 2005. Allelic relationships for genes that confer resistance to angular leaf spot in common bean. Euphytica, 145(3): 237-245.
10. Corrêa, R.X., Good-God, P.I., Oliveira, M.L., Nietsche, S., Moreira, M.A., Barros, E.G., 2001. Inheritance of resistance to the common bean angular leaf spot and identification of molecular markers flanking the resistance locus. Fitopatologia Brasileira, 26(1): 27-32.
11. Correa-Victoria, F.J., Pastor-Corrales, M.A., Saettler, A.W., 1989. Angular leaf spot. In: H.F. Schwartz and M.A. Pastor-Corrales (Eds.), Bean Production Problems in the Tropics, CIAT, Cali, Colombia, pp. 59-75.
12. Ddamulira, G., Mukankusi, C., Edema, R., Sseruwagi, P., Gepts, P., 2014. Identification of new sources of resistance to angular leaf spot among Uganda common bean landraces. Canadian Journal of Plant Breeding, 2(2): 55-65.
13. Ddamulira, G., Mukankusi, C., Ochwo-Ssemakula, M., Edema, R., Sseruwagi, P., Gepts, P., 2015. Gene pyramiding improved resistance to angular leaf spot in common bean. American Journal of Experimental Agriculture, 9(2): 1-12.
14. de Carvalho, G.A., Paula Junior, T.J., Alzate-Marin, A.L., Nietsche, S., Barros, E.G., Moreira, M.A., 1998. Inheritance of resistance of the Andean bean line AND-277 to race 63-23 of Phaeoisariopsis griseola and identification of a RAPD marker linked to the resistance gene. Fitopatologia Brasileira, 23: 482-485.
15. de Jesus, W.C., do Vale, F.X.R., Coelho, R.R., Hau, B., Zambolim, L., Costa, L.C., Filho, A.B., 2001. Effects of angular leaf spot and rust on yield loss of Phaseolus vulgaris. Phytopathology, 91(11): 1045-1053.
16. de Queiroz, V.T., de Sousa, C.S., Costa, M.R., Sanglad, D.A., Arruda, K.M.A., de Souza, T.L.P.O., Ragagnin, V.A., de Barros, E.G., Moreira, M.A., 2004. Development of SCAR markers linked to common bean angular leaf spot resistance genes. Annual Report-Bean Improvement Cooperative, 47: 237-238.
17. Ferreira, C.F., Borém, A., Carvalho, G.A., Nietsche, S., Paula, T.J., Jr., Barros, E.G., Moreira, M.A., 2000. Inheritance of angular leaf spot resistance in common bean and identifica¬tion of a RAPD marker linked to a resistance gene. Crop Science, 40(4): 1130-1133.
18. Gil, J., Solarte, D., Lobaton, J.D., Mayor, V., Barrera, S., Jara, C., Raatz, B., 2019. Fine-mapping of angular leaf spot resistance gene Phg-2 in common bean and development of molecular breeding tools. Theoretical and Applied Genetics, 132(7): 2003-2016.
19. Gonçalves-Vidigal, M.C., Cruz, A.S., Garcia, A., Kami, J., Vidigal Filho, P.S., Sousa, L.L., 2011. Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277. Theoretical and Applied Genetics, 122(5): 893-903.
20. Gonçalves-Vidigal, M.C., Cruz, A.S., Lacanallo, G.F., Vidigal Filho, P.S., Sousa, L.L., Pacheco, C.M.N.A., Pastor-Corrales, M.A., 2013. Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro. Theoretical and Applied Genetics, 126(9): 2245-2255.
21. Hall, R., 1994. Compendium of bean diseases. In: R. Hall (Ed.), Infectious Diseases, APS Press, Minnesota, pp. 15-16.
22. Johnson, E., Miklas, P.N., Stavely, J.R., Martinez-Cruzado, J.C., 1995. Coupling-and repulsion-phase RAPDs for marker-assisted selection of PI 181996 rust resistance in common bean. Theoretical and Applied Genetics, 90(5): 659-664.
23. Keller, B., Manzanares, C., Jara, C., Lobaton, J.D., Studer, B., Raatz, B., 2015. Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics, 128(5): 813-826.
24. Mahuku, G.S., Henríquez, M.A., Montoya, C., Jara, C., Teran, H., Beebe, S., 2011. Inheritance and development of molecular markers linked to angular leaf spot resistance genes in the common bean accession G10909. Molecular Breeding, 28(1): 57-71.
25. Mahuku, G.S., Iglesias, Á.M., Jara, C., 2009. Genetics of angular leaf spot resistance in the Andean common bean accession G5686 and identification of markers linked to the resistance genes. Euphytica, 167(3): 381-396.
26. Mahuku, G.S., Jara, C., Cuasquer, J.B., Castellanos, G., 2002. Genetic variability within Phaeoisariopsis griseola from Cen¬tral America and its implications for resistance breeding of common bean. Plant Pathology, 51(5): 594-604.
27. Mahuku, G.S., Montoya, C., Henríquez, M.A., Jara, C., Teran, H., Beebe, S., 2004. Inheritance and characterization of angu¬lar leaf spot resistance gene present in common bean acces¬sion G 10474 and identification of an AFLP marker linked to the resistance gene. Crop Science, 44(5): 1817-1824.
28. Miklas, P.N., Larsen, R.C., Riley, R., Kelly, J.D., 2000. Potential marker-assisted selection for bc-1 2 resistance to beancommon mosaic potyvirus in common bean. Euphytica, 116(3): 211-219.
29. Namayanja, A., Buruchara, R., Mahuku, G., Rubaihayo, P., Kimani, P., Mayanja, S., Eyedu, H., 2006. Inheritance of resistance to angular leaf spot in common bean and validation of the utility of resistance linked markers for marker assisted selection outside the mapping population. Euphytica, 151(3): 361-369.
30. Nay, M.M., Souza, T.L., Raatz, B., Mukankusi, C.M., Gonçalves-Vidigal, M.C., Abreu, A.F., Pastor-Corrales, M.A., 2019. A review of angular leaf spot resistance in common bean. Crop Science, 59(4): 1376-1391.
31. Nietsche, S., Borém, A., Assis De Carvalhos, G., De Paula Júnior, T.J., Fortes Ferreira, C., Gonçalves De Barros, E., Alves Moreira, M., 2001. Genetic diversity of Phaeoisariopsis grise¬ola in the state of Minas Gerais, Brazil. Euphytica, 117(1): 77-84.
32. Nietsche, S., Borém, A., Carvalho, G.A., Rocha, R.C., Paula, T.J., De Barros, E.G., Moreira, M.A., 2000. RAPD and SCAR markers linked to a gene conferring resistance to angular leaf spot in common bean. Journal of Phytopathology-Phytopathologische Zeitschrift, 148(2): 117-121.
33. Oblessuc, P.R., Baroni, R.M., Garcia, A.A.F., Chioratto, A.F., Carbonell, S.A.M., Camargo, L.E.A., Benchimol, L.L., 2012. Mapping of angular leaf spot resistance QTL in com¬mon bean (Phaseolus vulgaris L.) under different environments. BMC Genetic, 13(1): 1-9.
34. Padder, B.A., Sharma, P.N., Awale, H.E., Kelly, J.D., 2017. Colletotrichum lindemuthianum, the causal agent of bean anthracnose. Journal of Plant Pathology, 99(2): 317-330.
35. Palacıoğlu, G., 2021. Evaluatıon of Phg-resistance genes in common bean cultivars in Turkey to Pseudocercospora griseola, the causal agent of ALS disease. III. Balkan Agrıcultural Congress, 29 August-01 September, Edirne, Turkey, pp. 144.
36. Pastor-Corrales, M.A., Jara, C., Singh, S.P., 1998. Pathogenic variation in, source of, and breeding for resistance to Phaeoisariopsis griseola causing angular leaf spot in common bean. Euphytica, 103(2): 161-171.
37. Ragagnin, V.A., Sanglard, D.A., de Souza, T.L.P.O., Costa, M.R., Moreira, M.A., de Barros, E.G., 2005. A new inoculation procedure to evaluate angular leaf spot disease in bean plants (Phaseolus vulgaris L.) for breeding purposes. Annual Report-Bean Improvement Cooperative, 48: 90-91.
38. Rodríguez, D., Beaver, J., Jensen, C.E.D., Porch, T., 2019. Identification of resistance sources of common bean (Phaseolus vulgaris L.) to angular leaf spot (Pseudocercospora griseola). Revista Facultad Nacional de Agronomía Medellín, 72(2): 8785-8791.
39. Sanglard, D.A., Ribeiro, C.A.G., Balbi, B.P., Arruda, K.M.A., de Barros, E.G., Moreira, M.A., 2013. Characterization of the angular leaf spot resistance gene present in common bean cultivar Ouro Negro. Journal of Agricultural Science, 5(2): 19-23.
40. Sartorato, A., Alzate-Marin, A.L., 2004. Analysis of the pathogenic variability of Phaeoisariopsis griseola in Brazil. Annual Report of the Bean Improvement Cooperative, 47: 235-237.
41. Sartorato, A., Nietsche, S., Barros, E.G., Moreira, M.A., 1999. Inheritance of angular leaf spot resistance and RAPD mark¬ers linked to disease resistance gene in common beans. Annual Report of the Bean Improvement Cooperative, 42: 21-22.
42. Schmutz, J., McClean, P.E., Mamidi, S., Wu, G.A., Cannon, S.B., Grimwood, J., 2014. A reference genome for common bean and genome-wide analysis of dual domestications. Nature Genetics, 46(7): 707-713.
43. Stenglein, S., Ploper, L.D., Vizgarra, O., Balatti, P., 2003. Angular leaf spot: a disease caused by the fungus Phaeiosariopsis griseola (Sacc.) Ferraris on Phaseolus vulgaris L. Advances in Applied Microbiolology, 52: 209-243.
44. Teixeira Caixeta, E., Borém, A., de Azevedo Fagundes, S., Niestche, S., Gonçalves de Barros, E., Alves Moreira, M., 2003. Inheritance of angular leaf spot resistance in common bean line BAT 332 and identification of RAPD markers linked to the resistance gene. Euphytica, 134(3): 297-303.