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Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods

Year 2026, Volume: 13 Issue: 1, 44 - 49, 14.03.2026
https://doi.org/10.19159/tutad.1824914
https://izlik.org/JA58XJ84NX

Abstract

This study aimed to investigate and compare the phosphorus (P) extracted using four extraction methods (Bray-1, Mehlich-1, Hunter, and Fe/Al-Pi extraction methods). For this purpose, the authors analyzed P in a field experiment at the Federal University of Agriculture, Abeokuta conducted over a twelve-week period in the wet season. Analysis for available P was conducted on soil samples at 0, 4, 8, and 12 weeks after beddings (WAB). Data collected were subjected to regression analyses and analysis of variance (ANOVA); Duncan's Multiple Range Test separated significant means at 5% probability levels. Study findings demonstrate the effects of using different extractants. The highest amount of soil-extracted P was linked to the Mehlich-1 solution, followed by the Hunter solution, the Bray-1 solution, and Fe/Al-Pi. Significantly positive regression (p<0.001) relationships were established amongst chemical extractants and ion-sink methods. The strongest relationship was found between Fe/Al-Pi and Bray-1 (R2= 0.82). In conclusion, the Fe/Al-Pi ion-sink method provides a dependable alternative to Bray-1 for estimating P availability and assessing the phosphorus status of southwestern Nigerian soils.

References

  • Adepetu, J.A., Adebusuyi, B.A., 1985. Available data base for soil testing programme in Nigeria and further requirement for its development. Workshop on Soil Fertility Survey of Nigeria, 9-10 July, Jos, Nigeria, pp. 15-20.
  • Arai, Y., Sparks, D.L., 2007. Phosphate reaction dynamics in soils and soil minerals: A multiscale approach. Advances in Agronomy, 94: 135-179.
  • Azeez, J.O., Inyang, U.U., Olubuse, O.C., 2013. Determination of appropriate soil test extractant for available phosphorus in southwestern Nigeria soils. Communications in Soil Science and Plant Analysis, 44(10): 1540-1556.
  • Babana, A.H., Antoun, H., 2006. Effect of Tilemsi phosphate rock-solubilizing microorganism on phosphorus uptake and yield of field-grown wheat (Triticum aestivum L.) in Mali. Plant and Soil, 287(1-2): 51-58.
  • Bouyoucos, G.N., 1951. A recalibration of the hydrometer method for making mechanical analysis of soil. Journal of Agronomy, 43(9): 434-438.
  • Bray, R.H., Kurtz, L.T., 1945. Determination of total, organic, and available form of phosphorus in soils. Soil Science Society of America Journal, 59(1): 39-45.
  • De Araújo, L., Sampaio, E., De Araújo, M., 2015. Phosphorus desorption from Fe and Al oxides mediated by soil microorganisms. Communications in Soil Science and Plant Analysis, 46(5): 633-640.
  • Devau, N., Le Cadre, E., Hinsinger, P., Gérard, F., 2010. A mechanistic model for understanding root-induced chemical changes controlling phosphorus availability. Annals of Botany (London), 105(7): 1183-1197.
  • Hunter, A.H., 1974. Tentative ISFEJ Soil Extractant Procedure: International Soil Fertility Evaluation and Improvement Project. North Carolina State University, Raleigh, NC.
  • Jackson, M.L., 1964. Soil Chemical Analysis. Prentice-Hall, Inc., Englewood Cliffs, NJ, pp. 86-92.
  • Jackson, M.L., 1973. Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd., New Delhi.
  • Kweon, G., Lund, E., Maxton, C., Lee, W., Mengel, D., 2015. Comparison of soil phosphorus measurements. The American Society of Agricultural and Biological Engineers, 58(2): 405-414.
  • Lynch, J.P., 2011. Root phenes for enhanced soil exploration and phosphorus acquisition: Tools for future crops. Plant Physiology, 156(3): 1041-1049.
  • McLean, E.O., 1982. Chemical equilibrium with soil buffer systems as bases for future soil testing programs. Communications in Soil Science and Plant Analysis, 13(6): 411-433.
  • Meetei, W.H., Athokpam, H.S., Singh, R.K.K., Watham, L., Chongtham, N., Devi, K.N., Singh, N.B., Singh, K.L., Singh, N.J., 2015. Evaluation of some soil test methods in acid soils for available phosphorus for soybean of Imphal east district, Manipur, India. African Journal of Agricultural Resources, 10(8): 767-771.
  • Mehlich, A., 1953. Determination of P, K, Na, Mg, and Nh4. Raleigh: North Carolina Department of Agriculture, Soil Testing Division Publication No. 1-53, Raleigh, NC.
  • Menon, R.G., Chien, S.H., Hammond, L.L., 1989. Comparison of bray i and pi tests for evaluating plant-available phosphorus from soils treated with different partially acidulated phosphate rocks. Plant and Soil, 114(2): 211-216.
  • Morel, C., Ziadi, N., Messiga, A., 2014. Modeling of phosphorus dynamics in contrasting agroecosystems using long-term field experiments. The Canadian Journal of Soil Science, 94(3): 377-387.
  • Nelson, D.W., Sommers, L.E., 1996. Total carbon, organic carbon, and organic matter. In: A.L. Page, (Ed.), Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties, 2nd Edition, ASA, SSSA, Madison, WI, pp. 961-1010.
  • Oelkers, E.H., Valsami-Jones, E., 2008. Phosphate mineral reactivity and global sustainability. Elements, 4(2): 83-87.
  • Özyazıcı, M.A., 2024. Importance of phosphorus fertilization in legume forage crops. 8th International Conference on Global Practice of Multidisciplinary Scientific Studies, September 25-29, Dubai, pp. 1296-1317. (In Turkish).
  • Rouached, H., Arpat, A.B., Poirier, Y., 2010. Regulation of phosphate starvation responses in plants: signaling players and cross-talks. Molecular Plant, 3(2): 288-299.
  • Ruttenberg, K.C., 2009. The global phosphorus cycles. Treatise on Geochemistry, 9(13): 585-591.
  • Singh, B.R., Krogstad, T., Shivay, Y.S., Shivakumar, B.G., Bakkegard, M., 2005. Phosphorus fractionation and sorption in p-enriched soils of Norway. Nutrient Cycling in Agroecosystems, 73(2-3): 245-256.
  • Smil, V., 2000. Phosphorus in the environment: natural flows and human interferences. Annual Review of Energy and the Environment, 25(1): 53-88.
  • Zheng, Z.M., Zhang, T.Q., 2012. Soil phosphorus tests and transformation analysis to quantify plant availability. In: J.K. Whalen, (Ed.), Soil Fertility Improvement and Integrated Nutrient Management: A Global Perspective, InTech, Rijeka, Croatia, pp. 9-36.
  • Ziadi, N., Simard, R.R., Tran, T.S., Allard, G., 2001. Soil available phosphorus as evaluated by desorption techniques and chemical extractions. Canadian Journal of Soil Science, 81(2): 167-174.

Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods

Year 2026, Volume: 13 Issue: 1, 44 - 49, 14.03.2026
https://doi.org/10.19159/tutad.1824914
https://izlik.org/JA58XJ84NX

Abstract

This study aimed to investigate and compare the phosphorus (P) extracted using four extraction methods (Bray-1, Mehlich-1, Hunter, and Fe/Al-Pi extraction methods). For this purpose, the authors analyzed P in a field experiment at the Federal University of Agriculture, Abeokuta conducted over a twelve-week period in the wet season. Analysis for available P was conducted on soil samples at 0, 4, 8, and 12 weeks after beddings (WAB). Data collected were subjected to regression analyses and analysis of variance (ANOVA); Duncan's Multiple Range Test separated significant means at 5% probability levels. Study findings demonstrate the effects of using different extractants. The highest amount of soil-extracted P was linked to the Mehlich-1 solution, followed by the Hunter solution, the Bray-1 solution, and Fe/Al-Pi. Significantly positive regression (p<0.001) relationships were established amongst chemical extractants and ion-sink methods. The strongest relationship was found between Fe/Al-Pi and Bray-1 (R2= 0.82). In conclusion, the Fe/Al-Pi ion-sink method provides a dependable alternative to Bray-1 for estimating P availability and assessing the phosphorus status of southwestern Nigerian soils.

References

  • Adepetu, J.A., Adebusuyi, B.A., 1985. Available data base for soil testing programme in Nigeria and further requirement for its development. Workshop on Soil Fertility Survey of Nigeria, 9-10 July, Jos, Nigeria, pp. 15-20.
  • Arai, Y., Sparks, D.L., 2007. Phosphate reaction dynamics in soils and soil minerals: A multiscale approach. Advances in Agronomy, 94: 135-179.
  • Azeez, J.O., Inyang, U.U., Olubuse, O.C., 2013. Determination of appropriate soil test extractant for available phosphorus in southwestern Nigeria soils. Communications in Soil Science and Plant Analysis, 44(10): 1540-1556.
  • Babana, A.H., Antoun, H., 2006. Effect of Tilemsi phosphate rock-solubilizing microorganism on phosphorus uptake and yield of field-grown wheat (Triticum aestivum L.) in Mali. Plant and Soil, 287(1-2): 51-58.
  • Bouyoucos, G.N., 1951. A recalibration of the hydrometer method for making mechanical analysis of soil. Journal of Agronomy, 43(9): 434-438.
  • Bray, R.H., Kurtz, L.T., 1945. Determination of total, organic, and available form of phosphorus in soils. Soil Science Society of America Journal, 59(1): 39-45.
  • De Araújo, L., Sampaio, E., De Araújo, M., 2015. Phosphorus desorption from Fe and Al oxides mediated by soil microorganisms. Communications in Soil Science and Plant Analysis, 46(5): 633-640.
  • Devau, N., Le Cadre, E., Hinsinger, P., Gérard, F., 2010. A mechanistic model for understanding root-induced chemical changes controlling phosphorus availability. Annals of Botany (London), 105(7): 1183-1197.
  • Hunter, A.H., 1974. Tentative ISFEJ Soil Extractant Procedure: International Soil Fertility Evaluation and Improvement Project. North Carolina State University, Raleigh, NC.
  • Jackson, M.L., 1964. Soil Chemical Analysis. Prentice-Hall, Inc., Englewood Cliffs, NJ, pp. 86-92.
  • Jackson, M.L., 1973. Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd., New Delhi.
  • Kweon, G., Lund, E., Maxton, C., Lee, W., Mengel, D., 2015. Comparison of soil phosphorus measurements. The American Society of Agricultural and Biological Engineers, 58(2): 405-414.
  • Lynch, J.P., 2011. Root phenes for enhanced soil exploration and phosphorus acquisition: Tools for future crops. Plant Physiology, 156(3): 1041-1049.
  • McLean, E.O., 1982. Chemical equilibrium with soil buffer systems as bases for future soil testing programs. Communications in Soil Science and Plant Analysis, 13(6): 411-433.
  • Meetei, W.H., Athokpam, H.S., Singh, R.K.K., Watham, L., Chongtham, N., Devi, K.N., Singh, N.B., Singh, K.L., Singh, N.J., 2015. Evaluation of some soil test methods in acid soils for available phosphorus for soybean of Imphal east district, Manipur, India. African Journal of Agricultural Resources, 10(8): 767-771.
  • Mehlich, A., 1953. Determination of P, K, Na, Mg, and Nh4. Raleigh: North Carolina Department of Agriculture, Soil Testing Division Publication No. 1-53, Raleigh, NC.
  • Menon, R.G., Chien, S.H., Hammond, L.L., 1989. Comparison of bray i and pi tests for evaluating plant-available phosphorus from soils treated with different partially acidulated phosphate rocks. Plant and Soil, 114(2): 211-216.
  • Morel, C., Ziadi, N., Messiga, A., 2014. Modeling of phosphorus dynamics in contrasting agroecosystems using long-term field experiments. The Canadian Journal of Soil Science, 94(3): 377-387.
  • Nelson, D.W., Sommers, L.E., 1996. Total carbon, organic carbon, and organic matter. In: A.L. Page, (Ed.), Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties, 2nd Edition, ASA, SSSA, Madison, WI, pp. 961-1010.
  • Oelkers, E.H., Valsami-Jones, E., 2008. Phosphate mineral reactivity and global sustainability. Elements, 4(2): 83-87.
  • Özyazıcı, M.A., 2024. Importance of phosphorus fertilization in legume forage crops. 8th International Conference on Global Practice of Multidisciplinary Scientific Studies, September 25-29, Dubai, pp. 1296-1317. (In Turkish).
  • Rouached, H., Arpat, A.B., Poirier, Y., 2010. Regulation of phosphate starvation responses in plants: signaling players and cross-talks. Molecular Plant, 3(2): 288-299.
  • Ruttenberg, K.C., 2009. The global phosphorus cycles. Treatise on Geochemistry, 9(13): 585-591.
  • Singh, B.R., Krogstad, T., Shivay, Y.S., Shivakumar, B.G., Bakkegard, M., 2005. Phosphorus fractionation and sorption in p-enriched soils of Norway. Nutrient Cycling in Agroecosystems, 73(2-3): 245-256.
  • Smil, V., 2000. Phosphorus in the environment: natural flows and human interferences. Annual Review of Energy and the Environment, 25(1): 53-88.
  • Zheng, Z.M., Zhang, T.Q., 2012. Soil phosphorus tests and transformation analysis to quantify plant availability. In: J.K. Whalen, (Ed.), Soil Fertility Improvement and Integrated Nutrient Management: A Global Perspective, InTech, Rijeka, Croatia, pp. 9-36.
  • Ziadi, N., Simard, R.R., Tran, T.S., Allard, G., 2001. Soil available phosphorus as evaluated by desorption techniques and chemical extractions. Canadian Journal of Soil Science, 81(2): 167-174.
There are 27 citations in total.

Details

Primary Language English
Subjects Plant Nutrition and Soil Fertility, Soil Sciences and Plant Nutrition (Other)
Journal Section Research Article
Authors

Melvis Anamezeonye 0000-0002-5509-7765

Susan Loya 0000-0002-9916-2143

Bankole Olawale 0000-0003-4365-7451

Submission Date November 19, 2025
Acceptance Date March 6, 2026
Publication Date March 14, 2026
DOI https://doi.org/10.19159/tutad.1824914
IZ https://izlik.org/JA58XJ84NX
Published in Issue Year 2026 Volume: 13 Issue: 1

Cite

APA Anamezeonye, M., Loya, S., & Olawale, B. (2026). Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods. Türkiye Tarımsal Araştırmalar Dergisi, 13(1), 44-49. https://doi.org/10.19159/tutad.1824914
AMA 1.Anamezeonye M, Loya S, Olawale B. Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods. Türkiye Tarımsal Araştırmalar Dergisi. 2026;13(1):44-49. doi:10.19159/tutad.1824914
Chicago Anamezeonye, Melvis, Susan Loya, and Bankole Olawale. 2026. “Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods”. Türkiye Tarımsal Araştırmalar Dergisi 13 (1): 44-49. https://doi.org/10.19159/tutad.1824914.
EndNote Anamezeonye M, Loya S, Olawale B (March 1, 2026) Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods. Türkiye Tarımsal Araştırmalar Dergisi 13 1 44–49.
IEEE [1]M. Anamezeonye, S. Loya, and B. Olawale, “Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods”, Türkiye Tarımsal Araştırmalar Dergisi, vol. 13, no. 1, pp. 44–49, Mar. 2026, doi: 10.19159/tutad.1824914.
ISNAD Anamezeonye, Melvis - Loya, Susan - Olawale, Bankole. “Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods”. Türkiye Tarımsal Araştırmalar Dergisi 13/1 (March 1, 2026): 44-49. https://doi.org/10.19159/tutad.1824914.
JAMA 1.Anamezeonye M, Loya S, Olawale B. Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods. Türkiye Tarımsal Araştırmalar Dergisi. 2026;13:44–49.
MLA Anamezeonye, Melvis, et al. “Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods”. Türkiye Tarımsal Araştırmalar Dergisi, vol. 13, no. 1, Mar. 2026, pp. 44-49, doi:10.19159/tutad.1824914.
Vancouver 1.Melvis Anamezeonye, Susan Loya, Bankole Olawale. Evaluation of Phosphorus Extraction Efficiency from Soils Using Iron-Aluminum Impregnated Paper, Bray P-1, Mehlich-1, and Hunter Methods. Türkiye Tarımsal Araştırmalar Dergisi. 2026 Mar. 1;13(1):44-9. doi:10.19159/tutad.1824914