The Effect of Different Pollination Methods on Seed Yield and Germination Features in Stevia rebaudiana Bertoni

Year 2015, , 114 - 117, 30.09.2015

Esra Uçar , Yaşar Özyiğit , Kenan Turgut

https://doi.org/10.19159/tutad.44673

Abstract

Pollination is a prerequisite system for reproductive of many plants and it is more important in self-compatible plants. Since, these plants need other flowers pollen for flower fertilization and seed production. In this study, the effects of different pollination methods (open/cross pollination, self-pollination with hand and control) on some features associated with seed production in Stevia rebaudiana were investigated. Stevia which belongs to the Asteraceae family and is used as sweetener has a self-incompatibility problem. In the experiment, ten plants which were planted in a row were covered with net in the field condition and five of them were selfed with hand pollination and remaining 5 plants were left as it is. Furthermore, five uncovered plants were left to cross-pollination by insects. At the end of the experiment, seed yield per plant, 1000 seed weight, black/filled seed rate, number of day to first germination and germination rate were determined in harvested seeds. According to the results, cross-pollination was more superior in respect to all features in Stevia. This status shows that insect population (especially bee) must be present in Stevia fields for successful seed production. 

The Effect of Different Pollination Methods on Seed Yield and Germination Features in Stevia rebaudiana Bertoni

Abstract

Pollination is a prerequisite system for reproductive of many plants and it is more important in self-compatible plants. Since, these plants need other flowers pollen for flower fertilization and seed production. In this study, the effects of different pollination methods (open/cross pollination, self-pollination with hand and control) on some features associated with seed production in Stevia rebaudiana were investigated. Stevia which belongs to the Asteraceae family and is used as sweetener has a self-incompatibility problem. In the experiment, ten plants which were planted in a row were covered with net in the field condition and five of them were selfed with hand pollination and remaining 5 plants were left as it is. Furthermore, five uncovered plants were left to cross-pollination by insects. At the end of the experiment, seed yield per plant, 1000 seed weight, black/filled seed rate, number of day to first germination and germination rate were determined in harvested seeds. According to the results, cross-pollination was more superior in respect to all features in Stevia. This status shows that insect population (especially bee) must be present in Stevia fields for successful seed production.

Keywords

Bee, self pollination, self-compatible, sweet herb, cross pollination

References

• Brading P, El-Gabbas A, Zalat S and Gilbert F 2009. Biodiversity Economics: The Value of Pollination Services to Egypt. Egyptian Journal of Biology, 11: 46-51.
• Brandle J E, Starratt A N and Gijzen M 1998. Stevia rebaudiana: Its biological, chemical and agricultural properties. Canadian Journal of Plant Science, 78: 527-536.
• Cerana M 2004. Flower morphology and pollination in Mikania (Asteraceae). Flora 199:168-177.
• Corbet S A, Williams I H and Osborne J L 1991. Bees and the pollination of crops and wild flowers in the European Community. Bee World 72:47-59.
• Delaplane K S and Mayer D F 2000. Crop pollination by bees. CAB International, Oxon, United Kingdom, pp. 344.
• Goettemoeller J and Ching A 1999. Seed germination in Stevia rebaudiana. In: J. Janick (ed.), Perspectives on new crops and new uses. ASHS Press, Alexandria, VA. pp. 510-511.
• Hein L 2009. The Economic value of the pollination service, a review across scales. The Open Ecology Journal, 2: 74-82.
• Hof L and Lange W 1998. The influence of insect pollination on yield components in Dimorphotheca pluvialis. Genetic Resources and Crop Evolution, 45:441-446.
• Ingrouille M and Eddie B 2006. Plants: evolution and diversity, Cambridge Univ. Press, New York, pp. 440.
• Isagi Y 2011. Significance of single-pollen genotyping in ecological research. In: Y. Isagi and Y. Suyama (eds.), single-pollen genotyping (Chapter 1), Ecological Research Monographs, 1-6. DOI 10.1007/978-4-431-53901-8_1
• Klein A M, Müller C, Hoehn P and Kremen C 2009. Understanding the role of species richness for crop pollination services, In: S. Naeem, D.E. Bunker, A. Hector, M. Loreau, C. Perrings, (ed.), Biodiversity, ecosystem functioning, and human wellbeing–An ecological and economic perspective, Oxford University Press , pp. 195-208.
• Kumuda C N 2006. Influence of plant growth regulators and nitrogen on regulation of flowering in stevia (Stevia rebaudiana Bert.). (Master thesis) Department of Crop Physiology College of Agriculture, Dharwad University of Agricultural Sciences, Dharwad.
• Madan S, Ahmad S, Singh G N, Kohli K, Kumar Y, Singh R and Garg M 2010. Stevia rebaudiana (Bert.) Bertoni-A review. International Journal of Production Research, 1:267-286.
• Mamood A N, Ray D T and Waller G D 1990. Seed set in Guayule (Parthenium argentatum, Asteraceae) in relation to insect pollination. Economic Botany, 44(4): 440-444.
• Midmore D J and Rank A H 2002. A new rural industry- Stevia to replace imported chemical sweeteners, RIRDC web publication, Project No. UCQ- 16A. https://rirdc.infoservices.com.au/downloads/W02-022.pdf (Accessed: 11.01.2013).
• Munguía-Rosas, M A, Ollerton J and Parra-Tabla V 2011. Phenotypic selection on flowering phenology and size in two dioecious plant species with different pollen vectors. Plant Species Biology, 26:205-212.
• Paalhaar J, Boot W J, Van Der Steen J J M and Calis J N M 2008. In-hive pollen transfer between bees enhances cross-pollination of plants. Proceedings of the section Experimental and Applied Entomology of the Netherlands Entomological Society, 19: 53-58.
• Ramesh K, Singh V and Ahuja P S 2007. Production potential of Stevia rebaudiana (Bert.) Bertoni. under intercropping systems. Archives of Agronomy Soil Science, 53(4):443-458.
• Yadav A K, Singh S, Dhyani D and Ahuja P S 2011. A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Canadian Journal of Plant Science, 91(1):1-27.