The Effect of Different Nitrogen Doses on Yield and Some Yield Components of Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang

Abstract

Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang which belongs to Lamiaceae (mint) family is a perennial, herbaceous and essential oil bearing plant. Its essential oil and extract could be used as antimicrobial and antioxidant agent. However, there is a limited number of reports on agronomy and chemical contents of Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang. In this study, effect of five different nitrogen doses (0, 50, 100, 150 and 200 kg ha-1 ) on some agronomic traits of Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang were examined in the conditions of Mediterranean climate in 2011 and 2012. All traits were significantly affected by the different nitrogen doses in the second year of the experiment. Higher nitrogen doses increased plant height, number of branches, fresh herbage yield, fresh leaf yield, dry herbage yield and dry leaf yield. On the other hand, the highest N dose (200 kg ha-1 ) did not give the highest values in terms of plant height, number of branches, fresh herbage yield and dry herbage yield. In the second year, 150 kg ha-1 nitrogen treatment was appeared to be better than other treatments.

Keywords

• Herbage
• Melissa officinalis subsp. altissima
• nitrogen
• yield

The Effect of Different Nitrogen Doses on Yield and Some Yield Components of Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang

Abstract

Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang which belongs to Lamiaceae (mint) family is a perennial, herbaceous and essential oil bearing plant. Its essential oil and extract could be used as antimicrobial and antioxidant agent. However, there is a limited number of reports on agronomy and chemical contents of Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang. In this study, effect of five different nitrogen doses (0, 50, 100, 150and 200 kg ha^-1) on some agronomic traits of Melissa officinalis subsp. L. altissima (Sibthr. et Smith) Arcang were examined in the conditions of Mediterranean climate in 2011 and 2012. All traits were significantly affected by the different nitrogen doses in the second year of the experiment. Higher nitrogen doses increased plant height, number of branches, fresh herbage yield, fresh leaf yield, dry herbage yield and dry leaf yield. On the other hand, the highest N dose (200 kg ha^-1) did not give the highest values in terms of plant height, number of branches, fresh herbage yield and dry herbage yield. In the second year, 150 kg ha^-1 nitrogen treatment was appeared to be better than other treatments.

Keywords

• Herbage
• Melissa officinalis subsp. altissima
• nitrogen
• yield

References

1. Akhondzadeh, S., Noroozian, M., Mohammadi, M., Ohadinia, S., Jamshidi, A.H., Khani, M., 2003. Melissa officinalis extract in the treatment of patients with mild to moderate Alzheimer's disease: A double blind, randomised, placebo controlled trial. Journal of Neurology, Neurosurgery Psychiatry, 74(7): 863-866.
2. Azizi, A., Yan, F., Honormeier, B., 2009. Herbage yield, essential oils content and composition of three oregano (Origanum vulgare L.) populations as affected by soil moisture regimes and nitrogen supply. Industrial Crops and Products, 29(2-3): 554-561.
3. Baranauskien, R., Venskutonis, P.R., Viskelis, P., Dambrauskien, E., 2003. Influence of nitrogen fertilizers on the yield and composition of Thyme (Thymus vulgaris). Journal of Agricultural and Food Chemistry, 51(26): 7751-7758.
4. Basta, A., Tzakou, O., Couladis, M., 2005. Composition of the leaves essential oil of Melissa officinalis s. I. from Greece. Flavour and Fragrance Journal, 20(6): 642-644.
5. Carnat, A.P., Carnat, A., Fraisse, D., Lamaison, J.L., 1998. The aromatic and polyphenolic composition of lemon balm (Melissa oficinalis L. subsp. oficinalis) tea. Pharmaceutica Acta Helvetiae, 72(5): 301-305.
6. Christensen, L.P., Peacock, W.L., 2000. Mineral nutrition and fertilization. In: Raisin Production Manual, University of California, Agricultural and Natural Resources Publication 3393, Oakland, CA., pp. 102-114.
7. Dawson, B.S.W., Franich, R.A., Meder, R., 1998. Essential oil of Melissa officinalis L. subsp. altissima (Sibthr. et Smith) Arcang. Flavour and Fragrance Journal, 3(4): 167-170.
8. Dimitrova, Z., Dimov, B., Manolova, N., Pancheva, S., Ilieva, D., Shishkov, S., 1993. Antiherpes effect of Melissa officinalis L. extracts. Acta Microbiologica Bulgarica, 29: 65-72.

9. Fialová, S., Tekel’ová, D., Mrlianová, M., Grancai, D., 2008. The determination of phenolics compounds and antioxidant activity of mints and balms cultivated in slovakia. Acta Facultatis Pharmaceuticae Universitatis Comenianae, 55: 96-102.
10. Hener, U., Faulhaber, S., Kreis, P., Monsandl, A., 1995. On the authenticity evaluation of balm oil (Melissa officinalis L.). Pharmazie, 50: 60-62.
11. Hohmann, J., Zupkó, I., Rédei, D., Csányi, M., Falkay, G., Máthé, I., Janicsák, G., 1999. Protective effects of the aerial parts of Salvia officinalis, Melissa officinalis and Lavandula angustifolia and their constituents against enzyme-dependent and enzyme-independent lipid peroxidation. Planta Medica, 65(6): 576-578.
12. Inugraha, Maghfoer, M.D., Widaryanto, E., 2014. Response of stevia (Stevia rebaudiana Bertoni M) to nitrogen and potassium fertilization. IOSR Journal of Agriculture and Veterinary Science, 7(10): 47-55.
13. Karioti, A., Skaltsa, H., Demetzos, C., Perdetzoglou, D., Economakis, C.D., Salem, A.B., 2003. Effect of nitrogen concentration of the nutrient solution on the volatile constituents of leaves of Salvia fruticosa Mill. in solution culture. Journal of Agricultural and Food Chemistry, 51(22): 6505-6508.
14. Katar, D., Gürbüz, B., 2008. The effect of different plant densities and nitrogen doses on drug leaf yield and some features of lemon balm (Melissa officinalis L.). Journal of Agricultural Sciences, 14(1): 78-81.
15. Kothari, S.K., Singh, U.B., 1995. The effect of row spacing and nitrogen fertilization on Scotch spearmint (Mentha gracillis Sole). Journal of Essential Oil Research, 7(3): 287-297.
16. Mencherini, T., Picerno, P., Scesa, C., Aquino, R., 2007. Triterpene, antioxidant, and antimicrobial compounds from Melissa officinalis. Journal of Natural Product, 70(12): 1889-1894.
17. Mimica-Dukic, N., Bozin, B., Sokovic, M., Simin, N., 2004. Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil. Journal of Agricultural and Food Chemistry, 52(9): 2485-2489.
18. Mitchell, A.R., Farris, N.A., 1996. Peppermint response to nitrogen fertilizer in an arid climate. Journal of Plant Nutrition and Soil Science, 19(6): 955-967.
19. Özgüven, M., Ayanoğlu, F., Özel, A., 2006. Effects of nitrogen rates and cutting times on the essential oil yield and components of Origanum syriacum L. var. bevanii. Journal of Agronomy and Crop Science, 5(1): 101-105.
20. Said-Al Ahl, H.A.H., Hasnaa, S.A., Hendawy, S.F., 2009. Effect of potassium humate and nitrogen fertilizer on herb and essential oil of oregano under different irrigation intervals. Journal of Applied Sciences, 2(3): 320-323.
21. Shah, S.H., 2004. Morphophysiological response of black cumin (Nigella sativa L.) to nitrogen, gibberellic acid and kinetin application. PhD Thesis, Aligarh Muslim University, Aligarh, India.
22. Sodre, A.C.B., Luz, J.M.Q., Haber, L.L., Marques, M.O.M., Rodrigues, C.R., Blank, A.F., 2012. Organic and mineral fertilization and chemical composition of lemon balm (Melissa officinalis) essential oil. Revista Brasileira de Farmacognosia, 22(1): 40-44.
23. Sotiropoulou, D.E., Karamanos, A.J., 2010. Field studies of nitrogen application on growth and yield of Greek oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart). Industrial Crops and Products, 32(3): 450-457.
24. Tagashira, M., Ohtake, Y., 1998. A new antioxidative 1,3-benzodioxole from Melissa officinalis. Planta Medica, 64(6): 555-558.
25. Tucker, A.O., DeBaggio, T., 2000. The Big Book of Herbs: A Comprehensive Illustrated Reference to Herbs of Flavor and Fragrance. Interweave Press, 1St Edition.
26. Van De Berg, T., Freundl, E., Czygan, F.C., 1997. Melissa officinalis subsp. altissima: Characteristics of a possible adulteration of lemon balm. Pharmazie, 52: 802-808.