Protective Effects of Carvacrol, Essential Composition of Thyme Plant, on Cyclophosphamide-induced Reproductive System Damage on Rats

Year 2017, , 171 - 175, 30.06.2017

Mustafa Cengiz , Yasemin Tekin Behçet İnal , Adnan Ayhancı

https://doi.org/10.19159/tutad.295505

Cited By: 1

Abstract

Cyclophosphamide (CP) is an alkylating drug widely used clinically for the treatment of cancer and non -
malignant diseases. However, the use of CP causes cytotoxicity in one or more tissues. In this study, it was aimed to
determine possible protective effects of carvacrol (Car) which is the main component of thyme plant with antioxidant
properties on CP (50 and 100 mg kg-1
) testis toxicity. In the study, 35 male Sprague-Dawley rats were divided into 5 gro up s
as 7 animals in each group. According to histological and biochemical findings, Car was found to provide meaningful
protection in the prevention of testicular damage caused by CP. According to the results of the research, carvacrol is a highly
effective antioxidant and cell protector. For this reason, Car may be an effective candidate for reducing side effects of
anticancer drugs in chemotherapy protocols.

Keywords

Cyclophosphamide, testis damage, carvacrol, cell protector, rat

Kekik Bitkisinin Temel Bileşeni Olan Karvakrolün Sıçanlarda Siklofosfamid Nedenli Üreme Sistemi Hasarı Üzerine Koruyucu Etkileri

Abstract

Siklofosfamid (CP) klinikte kanser ve non-malignant hastalıkların tedavisinde yaygın olarak kullanılan alkilleyici bir ilaçtır. Ancak CP kullanımı bir veya birden fazla dokuda sitotoksisiteye neden olmaktadır . Bu çalışmada CP (50 ve 100 mg kg-1) testis toksisitesi üzerine kekik bitkisinin temel bileşeni olan ve antioksidan özellikleri bilinen karvakrol (Car)’ün olası koruyucu etkilerini saptamak amaçlanmıştır. Çalışmada, Sprague-Dawley cinsi 35 adet erkek sıçan her grupta 7 hayvan olacak şekilde 5 gruba ayrılmıştır. Histolojik ve biyokimyasal bulgulara göre, CP nedenli testis hasarının önlenmesinde Car anlamlı bir koruma sağladığı saptanmıştır. Araştırma sonuçlarına göre elde edilen veriler, karvakrolün oldukça etkili bir antioksidan ve hücre koruyucu olduğunu göstermiştir. Bu nedenle Car, kemoterapi protokollerinde antikanser ilaçların yan etkilerinin azaltılmasında etkili bir aday olabilir.

Keywords

Siklofosfamid, testis hasarı, karvakrol, hücre koruyucu, sıçan

References

• Ayhancı, A., Uyar, R., Aral, E., Kabadere, S., Appak, S., 2008. Protective effect of zinc on cyclophosphamide-induced hematoxicity and urotoxicity. Biological Trace Element Research, 126(3): 186-193.
• Aksu, E.H., Kandemir, F.M., Altun, S., Küçükler, S., Çomaklı, S., Ömür, A.D., 2016. Ameliorative effect of carvacrol on cisplatin-induced reproductive damage in malerats. Journal of Biochemical and Molecular Toxicology, 30(10): 513-520.
• Büyüknacar, H.S., Kumcu, E.K., Göçmen, C., Önder, S., 2008. Effect of phosphodiesterase type 4 inhibitor rolipram on cyclophosphamide-induced cystitis in rats. European Journal of Pharmacology, 586(1-3): 293-299.
• Cetik, S., Ayhancı, A., Şahintürk, V., 2015. Protective effect of carvacrol against oxidative stress and heart injury in cyclophosphamide-induced cardiotoxicity in rat. Brazilian Archives of Biology and Technology, 58(4): 596-76.
• Daggülli, M., Dede, O., Utangaç, M.M., Bodakçi, M.N., Hatipoğlu, N.K., Penbegül, N., Sancaktutar, A.A., Bozkurt, Y., Türkçü, G., Yüksel, H., 2014. Protective effects of carvacrol against methotrexate-induced testicular toxicity in rats. International Journal of Clinical Experimental Medicine, 7(12): 5511-5516.
• Erel, O., 2005. A new automated colorimetric method form easuring total oxidant status. Clinical Biochemistry, 38(12): 1103-1111.
• Fraiser, L.H., Kanekal, S., Kehrer, J.P., 1991. Cyclophosphamide toxicity. Drugs, 42(5): 781-795.
• Güneş, S., Ayhanci, A., Şahintürk, V., Altay, D.U., Uyar, R., 2017. Carvacrol attenuates cyclophosphamide-induced oxidative stress in rat kidney. (doi: 10.1139/cjpp-2016-0450).
• Howell, S., Shalet, S., 1998. Gonadal damage from chemotherapy and radiotherapy. Endocrinology Metabolism Clinical of North America, 27(4): 927-943.
• İpek, E., Zeytinoğlu, H., Okay, S., Tüylü, B.A., Kürkcüoğlu, M., Başer, K.H.C., 2005. Genotoxicity and antigenotoxicity of origanum oil and carvacrol evaluated byames Salmanella/microsomal test. Food Chemistry, 93(3): 551-556.
• Katzung, B., Masters, S., Trevor, A., 2009. Basic & clinical pharmacology. McGrawill Medical, New York.
• Kayaalp, S.O., 1998. Kanser kemoterapisinin esasları ve antineoplastik ilaçlar. Rasyonel Tedavi Yönünden Tıbbi Farmakoloji, 1(8): 1007-1072.
• Kumar, K.B.H., Kuttan, R., 2004. Chemoprotective activity of an extract of phyllanthus amarus against cyclophosphamide-induced toxicity in mice. Phytmedicine, 12(6-7): 494-500.
• Mahmoud, A.M., Soilman, H.A., Abd El-Hameed, A.M., Abdel-Reheim, E.S., 2016. Wheat germ oil attenuates cyclophosphamide induced testicular injury in rats. World Journal of Pharmacy and Pharmaceutical Sciences, 5(5): 40-52.
• Pool, B.L., Bos, R.P., Niemeyer, U., Theuws, J.L.G., Schmahl, D., 1988. In vitro/in vivo effect of mesna on the genotoxicity ans oxicity of cyclophosphamide: A study aimed at clarifying the mechanism of mesnas anticarsinogenic activity. Toxicology Letters, 41(1): 49-56.
• Quinn, G., Keough, M. 2002. Experimental designand data analysis for biologists. Cambridge University Press, New York, USA.
• Senthilkumar, S., Devaki, T., Manohar, B.M., Babu, M.S., 2006. Effect of squalene on cyclophosphamide-induced toxicity. Clinica Chimitica Acta, 364(1-2): 335-42.
• Sokal, R., Rohlf, J., 1995. Biometry. W. H. Freeman & Co, New York, USA. Third Edition, 887.