Inoculation Techniques for Assessing Pathogenicity of Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum and Fusarium solani on Pepper Seedlings

Year 2018, , 1 - 8, 28.02.2018

İnci Güler Güney , Ertuğrul Güldür

https://doi.org/10.19159/tutad.310211

Cited By: 4

Abstract

In this study, surveys were carried out during 2015
and 2016 for wilt and root rot diseases
caused by Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum, and Fusarium solani in pepper fields in Adıyaman, Diyarbakır, Mardin
and Şanlıurfa provinces of Turkey. The purpose of this study was to evaluate
the effects of different inoculation
methods (root dip, soil infestation with wheat bran and soil infestation with
rice grain) on pathogenicities of R.
solani, M. phaseolina, F. oxysporum and F. solani on pepper seedlings. Inoculated pepper seedlings (cv.
İnan-3363) were left to grow for three months after transplanting under growth
chamber conditions. Inoculation of infective rice-grain was used to test
pathogenicity of all four fungi. Root dip inoculation method
was used for F.
solani and F.
oxysporum when the soil was infested with
wheat bran method for R. solani and M.
phaseolina inoculation. All tested isolates resulted in the stem and root
rot, leaf chlorosis and bruising. To test the pathogenicity of fungi, soil
infestation with rice grain inoculation was the most suitable method. All
tested fungi induced similar foliar
symptoms, root rot severity and caused a similar reduction in dry root weights
when rice-grain inoculum was used. With other inoculation methods, all
pathogens similarly affected root rot severity. Whereas, F. oxysporum was the least virulent pathogen among tested fungi
affecting foliar symptom severity; for fresh
root and plant weights, and dry root and plant weights; R. solani, M. phaseolina and F. solani were similarly virulent when
these parameters were used. The results of the present study may have a useful connotation to monitor pepper seedlings
against these pathogens. In conclusion,
we recommend rice-grain inoculation to test pathogenicities of R. solani, M. phaseolina, F. oxysporum and F. solanion various pepper
cultivars.

Keywords

Pathogenicity, soil-borne pathogens, pepper

Inoculation Techniques for Assessing Pathogenicity of Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum and Fusarium solani on Pepper Seedlings

Abstract

In this study, surveys were carried out during 2015 and 2016 for wilt and root rot diseases caused by Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum, and Fusarium solani in pepper fields in Adıyaman, Diyarbakır, Mardin and Şanlıurfa provinces of Turkey. The purpose of this study was to evaluate the effects of different inoculation methods (root dip, soil infestation with wheat bran and soil infestation with rice grain) on pathogenicities of R. solani, M. phaseolina, F. oxysporum and F. solani on pepper seedlings. Inoculated pepper seedlings (cv. İnan-3363) were left to grow for three months after transplanting under growth chamber conditions. Inoculation of infective rice-grain was used to test pathogenicity of all four fungi. Root dip inoculation method was used for F. solani and F. oxysporum when the soil was infested with wheat bran method for R. solani and M. phaseolina inoculation. All tested isolates resulted in the stem and root rot, leaf chlorosis and bruising. To test the pathogenicity of fungi, soil infestation with rice grain inoculation was the most suitable method. All tested fungi induced similar foliar symptoms, root rot severity and caused a similar reduction in dry root weights when rice-grain inoculum was used. With other inoculation methods, all pathogens similarly affected root rot severity. Whereas, F. oxysporum was the least virulent pathogen among tested fungi affecting foliar symptom severity; for fresh root and plant weights, and dry root and plant weights; R. solani, M. phaseolina and F. solani were similarly virulent when these parameters were used. The results of the present study may have a useful connotation to monitor pepper seedlings against these pathogens. In conclusion, we recommend rice-grain inoculation to test pathogenicities of R. solani, M. phaseolina, F. oxysporum and F. solanion various pepper cultivars. 

Keywords

Pathogenicity, Fusarium solani, F. oxysporum, Macrophomina phaseolina

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