This study was conducted to determine the antifungal activity of Mentha spicata L. essential oils against 5 different
isolates of Chickpea
Blight (Ascochyta rabiei) under in vitro
conditions. The antifungal activity assay was performed in 60 mm Petri dishes containing 10 ml Potato-Dextrose-Agar (PDA) media. 5 mm diameter
fungal discs were placed in the middle of Petri dishes. The essential
oils was added to the sterile drying papers stuck to the Petri dishes using micropipette at doses of 0 (control), 1, 3, 5 and 10 μl. Petri dishes sealed with parafilm were incubated for 14 days at 25 ° C. Measurements were made after completion of the incubation period and percent inhibition of mycelial growth was determined. In conclusion, different doses of M. spicata essential oil inhibited
fungus mycelial growth
at different ratios,
and 10 uL dose completely (100%) stopped mycelial
growth of fungus. As a result of this study, it has been determined that M. spicata
essential oils has a
strong antifungal activity
in vitro conditions.
Primary Language | Turkish |
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Journal Section | Research Article |
Authors | |
Publication Date | June 30, 2018 |
Published in Issue | Year 2018 |