Nohut Yanıklık Hastalığı [Ascochyta rabiei (Pass) Labr.]’nın Farklı İzolatlarına Karşı Mentha spicata L. Uçucu Yağının Antifungal Aktivitesinin Belirlenmesi

Abstract

Bu çalışma, nohut yanıklık hastalığı (Ascochyta rabiei)’nın 5 farklı izolatlarına karşı Mentha spicata L. uçucu yağının antifungal aktivitesinin in vitro koşullarda belirlenmesi amacıyla yürütülmüştür. Antifungal etkinlik denemesi, 10 ml Potato- Dekstroz-Agar (PDA) besi yeri içeren 60 mm çapındaki petrilerde gerçekleştirilmiştir. Petri kaplarının ortasına 5 mm çapında fungal diskler yerleştirilerek konulmuştur. Uçucu yağ, petri kapaklarına yapıştırılan steril kurutma kağıtları üzerine mikro pipet yardımıyla 0 (kontrol), 1, 3, 5 ve 10 μl dozlarında eklenmiştir. Parafilm ile kapatılan petri kapları 25 °C’de 14 gün boyunca inkübe edilmiştir. İnkübasyon süresi tamamlandıktan sonra ölçümler yapılmış ve miselyal gelişiminin engellenmesi yüzde olarak belirlenmiştir. Çalışma sonucunda, M. spicata uçucu yağının farklı dozları, fungusun miseliyal gelişimini faklı oranlarda engellediği ve 10 μl’lik dozunun fungusun miseliyal gelişimini tamamen (% 100) durdurduğu belirlenmiştir. Bu çalışma sonucunda, M. spicata’nın uçucu yağınının in vitro koşullarda güçlü bir antifungal aktiviteye sahip olduğu belirlenmiştir.

Keywords

• Nohut yanıklığı (Ascochyta rabiei),antifungal etki,uçucu yağ,Mentha spicata L.

Determination of Antifungal Activity of Mentha spicata L. Essential Oils Against Different Isolates of Chickpea Blight Disease [Ascochyta rabiei (Pass) Labr.]

Abstract

This study was conducted to determine the antifungal activity of Mentha spicata L. essential oils against 5 different isolates of Chickpea Blight (Ascochyta rabiei) under in vitro conditions. The antifungal activity assay was performed in 60 mm Petri dishes containing 10 ml Potato-Dextrose-Agar (PDA) media. 5 mm diameter fungal discs were placed in the middle of Petri dishes. The essential oils was added to the sterile drying papers stuck to the Petri dishes using micropipette at doses of 0 (control), 1, 3, 5 and 10 μl. Petri dishes sealed with parafilm were incubated for 14 days at 25 ° C. Measurements were made after completion of the incubation period and percent inhibition of mycelial growth was determined. In conclusion, different doses of M. spicata essential oil inhibited fungus mycelial growth at different ratios, and 10 uL dose completely (100%) stopped mycelial growth of fungus. As a result of this study, it has been determined that M. spicata essential oils has a strong antifungal activity in vitro conditions.

Keywords

• Antracnose (Ascochyta rabiei),antifungal effect,essential oil,Mentha spicata L.

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