MatK ve trnH-psbA Barkot Genleri Kullanılarak Bazı Bitki Taksonlarının Moleküler Olarak Sınıflandırılması

Year 2019, , 87 - 93, 28.02.2019

Behcet İnal , Mehmet Karaca

https://doi.org/10.19159/tutad.488296

Cited By: 2

Abstract

Canlıların sınıflandırılması ve canlı birliklerine
ait sınırların çizilmesi gözleme ve deneye dayalı sistemli bilgi üretmeye
başlanmasıyla birlikte karşılaşılan en karmaşık problemlerden biri olmuştur. Bu
amaçla araştırmacılar birçok kuram ve metot geliştirerek var olan canlı
çeşitliliğini saptamaya çalışmışlardır. Çekirdek kökenli barkot bölgeleri,
plastid kökenli barkot bölgelerine göre çok daha fazla bilgi içermesine karşın,
tek lokus kullanılarak barkotlama yapıldığında, farklı bitki gruplarını
karşılaştırabilmek için yeterli bilgiye sahip olunmamaktadır. Tüm bitki
türlerinde kullanılabilecek tek bir barkot bölgesi henüz mevcut değildir ve bu
nedenle farklı barkot bölgelerinin birlikte kullanılması, türlerin ayırt
edilebilme gücünü arttırabilmektedir. Çalışmanın ana hedefi, bitki moleküler
filogenetiğini konu alan çalışmalarda etkin olarak kullanılabilecek gen, gen
bölgesi ve gen sayısını değerlendirmektir. Bu çalışmada, 15 farklı bitki
ailesine ait toplam 60 bitki türüne ait filogenetik ilişkiyi değerlendirmek
için matK, ve trnH-psbA barkot genler
kullanılarak MAFFT (Multiple Alignment Using Fast Fourier Transform) yazılımı
ile diziler hizalanmış ve Bayesian metodu ile konsensus filogenetik ağaç elde
edilmiştir. Sonuçlar bitki moleküler filogenetik çalışmalarında matK gen dizilerinin trnH-psbA
gen dizilerine göre daha yüksek ardıl olasılık değerli ağaç üretebildiğini
göstermiştir. Ancak daha fazla genlerin çalışması ile olası filogenetik ilişki
daha da iyi bir şekilde tahmin edilebilir.

Keywords

Filogeni, moleküler sistematik, biyoinformatik, barkot gen

Molecular Classification of Some Plant Taxa Using MatK and trnH-psbA Barcode Genes

Abstract

The classification of living creatures and the
demarcation of living units have been one of the most complex problems
encountered as a result of observing experimental and systematic information.
For this purpose, researchers have tried to determine the diversity of living
creatures by developing many theories and methods. Although the nuclear genome
barcode regions contain much more information than the barcode regions of
plastid, they do not have enough information to compare different plant groups
when barcoding with a single locus. A single barcode region that can be used in
all plant species is not yet available, and therefore, the use of different
barcode regions may increase the distinguishing power of species. Main
objective of this study was to determine gene, gene region and numbers of genes
suitable for plant molecular phylogentic studies. In
this study, matK, and trnH-psbA
barcode genes were used to evaluate the phylogenetic relationship of 60 plant
species belonging to 15 different plant families. Sequences were aligned with
MAFFT (Multiple Alignment Using Fast Fourier Transform) software and a
consensus phylogenetic tree was constructed by the Bayesian method. Results
indicated that matK gene was
much more suitaable in comparison to trnH-psbA region since the use of matK produced trees with higher posterior
probability values. However, further studies clearly showed that increased
number of genes produced much better phylogenetic estimations.

Keywords

Phylogeny, molecular systematics, bioinformatics, barcode gene

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