Determination of Enzyme Inhibition Effects and Antioxidant Activities of Campanula lyrata Lam. subsp. lyrata Grown in Turkey

Abstract

The aim of this study was to investigate on the antioxidant and enzyme inhibitory activities of methanol and water extracts obtained from aerial parts of Campanula lyrata Lam. subsp. lyrata grown in Turkey. In vitro antioxidant activities of the extracts were tested using total phenol and flavonoid content, 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), iron chelation and β-Carotene/linoleic acid emulsion methods. As for the enzyme inhibitory activities of the extracts, acetylcholinesterase and butyrylcholinesterase enzymes associated with Alzheimer's disease; α-amylase and α-glucosidase enzymes associated with diabetes; the tyrosinase enzyme which have an important role in cosmetics as skin whitening were tested, and the 96-well plate method were used. According to the results, it was detected that methanol and water extracts of C. lyrata subsp. lyrata have antioxidant activities as comparable with the reference compound except for DPPH and iron chelation activity. In addition, it was observed that the extracts showed more prominent activity on α-amylase and tyrosinase enzymes, and had more inhibitory effects than other enzymes. As a result, our study has the quality to contribute to the further studies on C. lyrata subsp. lyrata for the investigation of the compounds responsible for the activity.

Keywords

• Campanula lyrata
• radical scavenger
• tyrosinase
• cholinesterase
• α-amylase
• α-glucosidase

Türkiye’de Yetişen Campanula lyrata Lam. subsp. lyrata’nın Enzim İnhibe Edici Etkilerinin ve Antioksidan Aktivitelerinin Belirlenmesi

Abstract

Bu çalışmanın amacı, Türkiye’de yetişen Campanula lyrata Lam. subsp. lyrata’nın toprak üstü kısımlarından elde edilen metanol ve su ekstrelerinin antioksidan ve enzim inhibe edici aktivitelerini araştırmaktır. Ekstrelerin in vitro antioksidan aktiviteleri toplam fenol ve flavonoit miktar tayini, 2-2´-difenil-1-pikirhidrazil (DPPH), 2,2'-azino-bis (3-etilbenzotiyazolin-6-sulfonik asit) (ABTS), demir şelasyon ve β-Karoten/linoleik asit emülsiyon yöntemleri kullanılarak test edilmiştir. Ekstrelerin enzim inhibe edici aktivitelerinde ise, Alzheimer hastalığıyla ilişkili olan asetilkolinesteraz ve butirilkolinesteraz enzimleri; diyabet hastalığıyla ilişkili α-amilaz ve α-glukozidaz enzimleri; cilt beyazlatıcı olarak kozmetikte önemli bir yere sahip olan tirozinaz enzimi test edilmiş ve 96-kuyucuklu plaka yöntemi kullanılmıştır. Araştırma sonuçlarına göre, C. lyrata subsp. lyrata’nın metanol ve su ekstrelerinin DPPH ve demir şelasyon aktivitesi dışında referans maddeyle karşılaştırılabilir düzeyde antioksidan aktivitelere sahip oldukları tespit edilmiştir. Ayrıca, ekstrelerin α-amilaz ve tirozinaz enzimleri üzerinde daha belirgin aktivite gösterdikleri ve diğer enzimlere kıyasla daha fazla inhibe edici etkilere sahip oldukları gözlenmiştir. Sonuç olarak çalışmamız, C. lyrata subsp. lyrata üzerinde aktiviteden sorumlu bileşiklerin araştırılmasına yönelik ilerideki çalışmalara katkı sağlayacak nitelik taşımaktadır.

Keywords

• Campanula lyrata
• radikal süpürücü
• tirozinaz
• kolinesteraz
• α-amilaz
• α-glukozidaz

Thanks

Deneysel çalışmaların gerçekleştirilmesine uygun ortam sağlayan Selçuk Üniversitesi Eczacılık Fakültesi ve Fen Fakültesi alt yapısından yararlanılmıştır. Sayın Doç. Dr. Nuraniye Eruygur ve Prof. Dr. Hayri Duman hocalarımıza desteklerinden dolayı da çok teşekkür ederim.

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