Increasing world population needs to enhance agricultural production because of food starvation. Genetically
modified organism (GMO) is a way to solve this problem. During gene transfers, DNA is inserted into a plant’s genome in a
random way. This produces spontaneous genetic changes with movement of transposable elements, and even increases
variations. Houba was described as one of the active retrotransposons in rice. The aim of this study was to screen rice
samples collected from Turkey, and analyse Houba retrotransposon movements with IRAP technique in transgenic ones and
their controls. For this purpose, 71 different rice seeds obtained from different regions of Turkey were used for GMO
analysis. All samples were screened by real time PCR to test cauliflower mosaic virus (CaMV) 35S promoter (P-35S)
regions, T-NOS (nopaline synthase terminator) regions, figwort mosaic virus (FMV) regions, bar, pat and Cry1ab/ac, and
hpt (hygromycin resistance) genes. Hpt gene was identified in 6 samples as a result of real time PCR analysis. These 6
transgenic samples with their controls were used for IRAP-PCR analysis and 0-56% polymorphism ratios were observed in
analysed samples. This study is one of the first detailed experimental data of transgenic Oryza sativa L. samples in terms of
retrotransposon-based variation.
Primary Language | English |
---|---|
Journal Section | Research Article |
Authors | |
Publication Date | February 28, 2017 |
Published in Issue | Year 2017 Volume: 4 Issue: 1 |