Romanov Koyunları Amniyon Sıvısı ile Hyalüronidaz Aktivitesinin Araştırılması

Year 2018, Volume: 5 Issue: 1, 58 - 64, 28.02.2018

Mustafa Oğuzhan Kaya , Kıvanç İrak , Seyrani Mersin , Emrah Yerlikaya , Hasan Karageçili

https://doi.org/10.19159/tutad.350951

Abstract

Sığır testis hyalüronidazı (Bovine Testicular
Hyaluronidase, BTH) endüstriyel alanda, kanser çalışmalarında ve birçok hayvan
uygulamalarında oldukça önemli bir enzimdir. Bu çalışmada, Romanov ırkı
koyunların amniyon sıvıları BTH enzimi için alternatif yeni bir substrat
kaynağı olarak kullanılıp kullanılamayacağı araştırılmıştır. Araştırma
sonucunda, ticari olarak satın alınan hyalüronik asit (HA) ile tespit edilen
BTH aktivitesi 9.187 U mL^-1 bulunurken, 35 farklı amniyotik keseden alınan sıvılar kullanılarak BTH
aktivitesi 1.604-18.659 U mL^-1 aralığında bulunmuştur. Romanov ırkı koyunların amniyon sıvısının HA
içeriğinden dolayı BTH enzimi için bir substrat kaynağı olup olmadığına dair
rapor edilen herhangi bir bilgiye rastlanmamıştır. Çalışma sonunda ticari HA
substrat olarak kullanıldığında hesaplanan BTH aktivitesi ile amniyon sıvıları
kullanılarak elde edilen BTH aktiviteleri oldukça yakın olduğundan, Romanov
ırkı koyunların amniyon sıvılarının yeni bir HA kaynağı olabileceği
düşünülmüştür.

Keywords

Romanov koyunu, hyalüronidaz, hyalüronik asit, amniyon sıvısı, substrat

Investigation of Hyaluronidase Activity with Amniotic Fluid on Romanov Sheep

Abstract

Bovine testicular hyaluronidase (BTH) is a very important enzyme in industrial field, cancer studies and in many
animal applications. In this study, it was investigated whether amniotic fluid of Romanov sheep could be used as an
alternative new substrate source for BTH enzyme. As a result of the study, the BTH activity was found as 9.187 U mL-1 with
commercially available hyaluronic acid (HA), while the BTH activity of 35 different amniotic fluids was found between
1.604-18.659 U mL-1
. No information was reported on whether the amniotic fluids of the Romanov breed sheep were a
source of substrate for the BTH enzyme due to the HA content. It was thought that amniotic fluid of the Romanov breed
sheep could be a new HA source, as the BTH activity calculated using the commercial HA and the BTH activities obtained
using amniotic fluid were very close.

Keywords

Romanov sheep, hyaluronidase, hyaluronic acid, amniotic fluid, substrate

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