Haplophyllum suaveolens Varyetelerinin Antioksidan Aktivitesi ve Sekonder Metabolitleri Üzerine Farklı Çözücülerin Etkisi

Year 2019, Volume: 6 Issue: 3, 277 - 284, 31.10.2019

Cennet Yaman , Deniz Ulukuş , Osman Tugay

https://doi.org/10.19159/tutad.590387

Cited By: 2

Abstract

Bu çalışmada, Haplophyllum
suaveolens varyetelerine ait methanol ve etanol ekstraktlarının toplam
biyoaktif içerikleri ile antioksidan aktivite seviyelerinin belirlenmesi
amaçlanmıştır. Ekstraksiyon işleminde Haplophyllum
suaveolens varyeteleri olan Haplophyllum
suaveolens (DC.) G.Don var. suaveolens
(HS) ve endemik Haplophyllum suaveolens
var. cilicicum (Boiss.) C.C.Townsend
(HC) taksonlarının herba kısımları kullanılmıştır. Toplam fenolik içerik
Folin-Ciocalteu metotuna göre belirlenmiş ve her bir ekstrakttaki miktarları mg
gallik asit eş değer (GAE) g^-1 ekstrakt olarak ifade edilmiştir.
Ekstraktların toplam fenolik içerikleri 39.54-44.16 mg GAE g^-1
ekstrakt arasında değişim göstermiştir. Toplam flavonoid içerikleri ise mg
kuersetin eş değer (QE) g^-1 ekstrakt olarak ölçülmüş ve 17.30-41.40
mg QE g^-1 ekstrakt arasında değişim göstermiştir. Çalışmada, HS ve
HC taksonlarının methanol ekstraktları en yüksek 2,2-difenil-1-pikrilhidrazil
(DPPH, sırayla % 79.76 ve % 75.69) ve 2,2’-azinobis (3-etil-bezotiazolin 6
sulfonat) (ABTS, sırayla % 54.74 ve % 55.13) radikal kovucu aktiviteleri
göstermiştir. Böylece, Haplophyllum
suaveolens varyetelerinin güçlü radikal kovucu aktivite sergilediği ve her
iki varyete içinde metanol ekstraktların etanol ekstraktlarına göre daha etkili
olduğu tespit edilmiştir. Bu çalşma ile Haplophyllum
suaveolens varyetelerinin güçlü radikal kovucu aktiviteye sahip olduğu,
tıbbi ve ticari kullanım için iyi birer doğal antioksidan kaynağı olabileceği
tespit edilmiştir.

Keywords

Haplophyllum L., Antioksidan aktivitesi, flavonoid, Haplophyllum L., fenolik

Effect of Different Solvents on Antioxidant Activities and Secondery Metabolites of Haplophyllum suaveolens Varieties

Abstract

In
this study, total bioactive contents and antioxidant activity levels of
methanol and ethanol extracts of Haplophyllum
suaveolens varieties were determined. Aerial parts of Haplophyllum
suaveolens varieties which are Haplophyllum suaveolens (DC.) G. Don var. suaveolens (HS) and endemic
Haplophyllum suaveolens var.
cilicicum (Boiss.) C.C. Townsend (HC), were used in the extraction
process. Total phenolic content was
determined according to the Folin-Ciocalteu method and the amounts in each
extract were expressed as mg gallic acid equivalent (GAE) g-1 extract. The total phenolic contents of the extracts
varied between 39.54-44.16 mg GAE g^-1 extract. Total flavonoid
contents were measured as mg quercetin equivalent (QE) g^-1 extract
and varied between 17.30-41.40 mg QE g^-1 extract. The methanol
extracts of HS and HC exhibited maximum radical scavenging activities for DPPH
radical (79.76% and 75.69%, respectively) and ABTS (54.74% and 55.13%,
respectively). Thus, Haplophyllum
suaveolens varieties exhibited strong radical scavenging activity and
methanol extracts were found to be more effective than ethanol extracts in both
varieties. In this study, it has been determined that Haplophyllum suaveolens varieties have strong radical scavenging
activity and maybe good natural antioxidant sources for medical and commercial
use.

Keywords

Haplophyllum L., Antioxidant activity, flavonoid, Haplophyllum L., phenolic

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