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Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)

Year 2022, Volume 9, Issue 3, 274 - 285, 31.10.2022
https://doi.org/10.19159/tutad.1109558

Abstract

The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools of in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.

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Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)

Year 2022, Volume 9, Issue 3, 274 - 285, 31.10.2022
https://doi.org/10.19159/tutad.1109558

Abstract

The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools of in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.

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Details

Primary Language English
Subjects Science
Journal Section Research Article
Authors

Ahmed Sidar AYGÖREN>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0002-6264-9935
Türkiye


Recep AYDINYURT>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0003-3743-1835
Türkiye


Sümeyra UÇAR>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0002-7629-0206
Türkiye


Ayşe Gül KASAPOĞLU>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0002-6447-4921
Türkiye


Esra YAPRAK>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0002-8753-494X
Türkiye


Burak Muhammed ÖNER>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0003-2785-2089
Türkiye


Selman MUSLU>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0003-4777-0726
Türkiye


Murat ISIYEL>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0003-4157-2729
Türkiye


Emre İLHAN> (Primary Author)
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0002-8404-7900
Türkiye


Murat AYDIN>
Atatürk University, Faculty of Agriculture, Department of Agricultural Biotechnology, Erzurum, TÜRKİYE
0000-0003-1091-0609
Türkiye


Murat TURAN>
Erzurum Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Erzurum, TÜRKİYE
0000-0003-2900-1755
Türkiye

Publication Date October 31, 2022
Published in Issue Year 2022, Volume 9, Issue 3

Cite

Bibtex @research article { tutad1109558, journal = {Türkiye Tarımsal Araştırmalar Dergisi}, issn = {2148-2306}, eissn = {2528-858X}, address = {}, publisher = {Siirt University}, year = {2022}, volume = {9}, number = {3}, pages = {274 - 285}, doi = {10.19159/tutad.1109558}, title = {Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)}, key = {cite}, author = {Aygören, Ahmed Sidar and Aydınyurt, Recep and Uçar, Sümeyra and Kasapoğlu, Ayşe Gül and Yaprak, Esra and Öner, Burak Muhammed and Muslu, Selman and Isıyel, Murat and İlhan, Emre and Aydın, Murat and Turan, Murat} }
APA Aygören, A. S. , Aydınyurt, R. , Uçar, S. , Kasapoğlu, A. G. , Yaprak, E. , Öner, B. M. , Muslu, S. , Isıyel, M. , İlhan, E. , Aydın, M. & Turan, M. (2022). Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.) . Türkiye Tarımsal Araştırmalar Dergisi , 9 (3) , 274-285 . DOI: 10.19159/tutad.1109558
MLA Aygören, A. S. , Aydınyurt, R. , Uçar, S. , Kasapoğlu, A. G. , Yaprak, E. , Öner, B. M. , Muslu, S. , Isıyel, M. , İlhan, E. , Aydın, M. , Turan, M. "Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)" . Türkiye Tarımsal Araştırmalar Dergisi 9 (2022 ): 274-285 <https://dergipark.org.tr/en/pub/tutad/issue/73473/1109558>
Chicago Aygören, A. S. , Aydınyurt, R. , Uçar, S. , Kasapoğlu, A. G. , Yaprak, E. , Öner, B. M. , Muslu, S. , Isıyel, M. , İlhan, E. , Aydın, M. , Turan, M. "Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)". Türkiye Tarımsal Araştırmalar Dergisi 9 (2022 ): 274-285
RIS TY - JOUR T1 - Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.) AU - Ahmed SidarAygören, RecepAydınyurt, SümeyraUçar, Ayşe GülKasapoğlu, EsraYaprak, Burak MuhammedÖner, SelmanMuslu, MuratIsıyel, Emreİlhan, MuratAydın, MuratTuran Y1 - 2022 PY - 2022 N1 - doi: 10.19159/tutad.1109558 DO - 10.19159/tutad.1109558 T2 - Türkiye Tarımsal Araştırmalar Dergisi JF - Journal JO - JOR SP - 274 EP - 285 VL - 9 IS - 3 SN - 2148-2306-2528-858X M3 - doi: 10.19159/tutad.1109558 UR - https://doi.org/10.19159/tutad.1109558 Y2 - 2022 ER -
EndNote %0 Turkish Journal of Agricultural Research Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.) %A Ahmed Sidar Aygören , Recep Aydınyurt , Sümeyra Uçar , Ayşe Gül Kasapoğlu , Esra Yaprak , Burak Muhammed Öner , Selman Muslu , Murat Isıyel , Emre İlhan , Murat Aydın , Murat Turan %T Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.) %D 2022 %J Türkiye Tarımsal Araştırmalar Dergisi %P 2148-2306-2528-858X %V 9 %N 3 %R doi: 10.19159/tutad.1109558 %U 10.19159/tutad.1109558
ISNAD Aygören, Ahmed Sidar , Aydınyurt, Recep , Uçar, Sümeyra , Kasapoğlu, Ayşe Gül , Yaprak, Esra , Öner, Burak Muhammed , Muslu, Selman , Isıyel, Murat , İlhan, Emre , Aydın, Murat , Turan, Murat . "Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)". Türkiye Tarımsal Araştırmalar Dergisi 9 / 3 (October 2022): 274-285 . https://doi.org/10.19159/tutad.1109558
AMA Aygören A. S. , Aydınyurt R. , Uçar S. , Kasapoğlu A. G. , Yaprak E. , Öner B. M. , Muslu S. , Isıyel M. , İlhan E. , Aydın M. , Turan M. Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). TÜTAD. 2022; 9(3): 274-285.
Vancouver Aygören A. S. , Aydınyurt R. , Uçar S. , Kasapoğlu A. G. , Yaprak E. , Öner B. M. , Muslu S. , Isıyel M. , İlhan E. , Aydın M. , Turan M. Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). Türkiye Tarımsal Araştırmalar Dergisi. 2022; 9(3): 274-285.
IEEE A. S. Aygören , R. Aydınyurt , S. Uçar , A. G. Kasapoğlu , E. Yaprak , B. M. Öner , S. Muslu , M. Isıyel , E. İlhan , M. Aydın and M. Turan , "Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)", Türkiye Tarımsal Araştırmalar Dergisi, vol. 9, no. 3, pp. 274-285, Oct. 2022, doi:10.19159/tutad.1109558

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