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Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)

Year 2022, Volume: 9 Issue: 3, 274 - 285, 31.10.2022
https://doi.org/10.19159/tutad.1109558

Abstract

The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools of in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.

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Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)

Year 2022, Volume: 9 Issue: 3, 274 - 285, 31.10.2022
https://doi.org/10.19159/tutad.1109558

Abstract

The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools of in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.

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Details

Primary Language English
Journal Section Research Article
Authors

Ahmed Sidar AYGÖREN 0000-0002-6264-9935

Recep AYDINYURT 0000-0003-3743-1835

Sümeyra UÇAR 0000-0002-7629-0206

Ayşe Gül KASAPOĞLU 0000-0002-6447-4921

Esra YAPRAK 0000-0002-8753-494X

Burak Muhammed ÖNER 0000-0003-2785-2089

Selman MUSLU 0000-0003-4777-0726

Murat ISIYEL 0000-0003-4157-2729

Emre İLHAN 0000-0002-8404-7900

Murat AYDIN 0000-0003-1091-0609

Murat TURAN 0000-0003-2900-1755

Publication Date October 31, 2022
Published in Issue Year 2022 Volume: 9 Issue: 3

Cite

APA AYGÖREN, A. S., AYDINYURT, R., UÇAR, S., KASAPOĞLU, A. G., et al. (2022). Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). Türkiye Tarımsal Araştırmalar Dergisi, 9(3), 274-285. https://doi.org/10.19159/tutad.1109558
AMA AYGÖREN AS, AYDINYURT R, UÇAR S, KASAPOĞLU AG, YAPRAK E, ÖNER BM, MUSLU S, ISIYEL M, İLHAN E, AYDIN M, TURAN M. Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). TÜTAD. October 2022;9(3):274-285. doi:10.19159/tutad.1109558
Chicago AYGÖREN, Ahmed Sidar, Recep AYDINYURT, Sümeyra UÇAR, Ayşe Gül KASAPOĞLU, Esra YAPRAK, Burak Muhammed ÖNER, Selman MUSLU, Murat ISIYEL, Emre İLHAN, Murat AYDIN, and Murat TURAN. “Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus Vulgaris L.)”. Türkiye Tarımsal Araştırmalar Dergisi 9, no. 3 (October 2022): 274-85. https://doi.org/10.19159/tutad.1109558.
EndNote AYGÖREN AS, AYDINYURT R, UÇAR S, KASAPOĞLU AG, YAPRAK E, ÖNER BM, MUSLU S, ISIYEL M, İLHAN E, AYDIN M, TURAN M (October 1, 2022) Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). Türkiye Tarımsal Araştırmalar Dergisi 9 3 274–285.
IEEE A. S. AYGÖREN, “Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.)”, TÜTAD, vol. 9, no. 3, pp. 274–285, 2022, doi: 10.19159/tutad.1109558.
ISNAD AYGÖREN, Ahmed Sidar et al. “Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus Vulgaris L.)”. Türkiye Tarımsal Araştırmalar Dergisi 9/3 (October 2022), 274-285. https://doi.org/10.19159/tutad.1109558.
JAMA AYGÖREN AS, AYDINYURT R, UÇAR S, KASAPOĞLU AG, YAPRAK E, ÖNER BM, MUSLU S, ISIYEL M, İLHAN E, AYDIN M, TURAN M. Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). TÜTAD. 2022;9:274–285.
MLA AYGÖREN, Ahmed Sidar et al. “Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus Vulgaris L.)”. Türkiye Tarımsal Araştırmalar Dergisi, vol. 9, no. 3, 2022, pp. 274-85, doi:10.19159/tutad.1109558.
Vancouver AYGÖREN AS, AYDINYURT R, UÇAR S, KASAPOĞLU AG, YAPRAK E, ÖNER BM, MUSLU S, ISIYEL M, İLHAN E, AYDIN M, TURAN M. Genome-Wide Analysis and Characterization of the PIF Gene Family Under Salt and Drought Stress in Common Beans (Phaseolus vulgaris L.). TÜTAD. 2022;9(3):274-85.

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