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Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress

Year 2025, Volume: 12 Issue: 2, 155 - 161, 30.06.2025

Abstract

This study aimed to investigate the effects of drought stress on melon (Cucumis melo L.) and to evaluate the potential mitigating role of gibberellic acid (GA₃) application. In the experiment, a 10-day drought period was imposed by withholding irrigation, and GA₃ was applied at a concentration of 100 ppm. The results indicated that drought stress significantly suppressed plant growth. The SPAD value decreased by 26%, from 303.6 ± 8.9 in the control group to 224.1 ± 5.9 under drought conditions, while GA₃ application did not lead to a statistically significant improvement. Drought stress increased malondialdehyde levels from 3.6 to 7.9, whereas GA₃ treatment reduced it to 5.67, indicating a partial alleviation of oxidative stress. Root length decreased from 50 ± 8.1 cm in the control to 31 ± 5.09 cm under drought stress and was further reduced to 25 ± 6.03 cm with GA₃ application. Principal Coordinate Analysis revealed that GA₃ application did not completely mitigate drought stress but provided partial improvement. These findings highlight the detrimental effects of drought stress on melon and indicate that GA₃ alone may not be sufficient to alleviate drought-induced damage in melon cultivation.

References

  • Ahmed, C.B., Rouina, B.B., Boukhris, M., 2007. Effects of water deficit on olive trees cv. Chemlali under field conditions in arid region in Tunisia. Scientia Horticulturae, 113: 267-277.
  • Coşkun, Ö.F., 2023. The effect of grafting on morphological, physiological and molecular changes induced by drought stress in cucumber. Sustainability, 15(1): 875.
  • Coşkun, Ö.F., 2025. Association mapping for drought tolerance in watermelons (Citrullus lanatus L.). Horticulturae, 11(2): 193.
  • Doruk Kahraman, N., Okumuş, O., 2024. Role of gibberellic acid (GA₃) in improving salt stress tolerance of wheat (Triticum aestivum). Journal of Erciyes Agriculture and Animal Science, 7(2): 86-93.
  • Fang, Y.J., Xiong, L.Z., 2015. General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and Molecular Life Sciences, 72(4): 673-689.
  • Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A., 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212.
  • Gebril, S., Elsayed, H.M.A., 2025. Effect of drought stress on seedling morphological traits of four commercial hybrids of Egyptian watermelon. International Journal of Emerging Technologies, 16(1): 1-7.
  • Giordano, M., Petropoulos, S.A., Rouphael, Y., 2021. Response and defense mechanisms of vegetable crops against drought, heat, and salinity stress. Agriculture, 11(5): 463.
  • Grafton, R.Q., Daugbjerg, C., Qureshi, M.E., 2015. Towards food security by 2050. Food Security, 7: 179-183.
  • Imran, M., Latif Khan, A., Shahzad, R., Aaqil Khan, M., Bilal, S., Khan, A., Kang, S.-M., Lee, I.-J., 2021. Exogenous melatonin induces drought stress tolerance by promoting plant growth and antioxidant defense system of soybean plants. AoB Plants, 13(4): plab026.
  • Jaleel, C.A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H.J., Somasundaram, R., Panneerselvam, R., 2009. Drought stress in plants: A review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology, 11(1): 100-105.
  • Karahara, I., Horie, T., 2021. Functions and structure of roots and their contributions to salinity tolerance in plants. Breeding Science, 71(1): 89-108.
  • Kaur, G., Kaur, A., 2016. Plant growth and fruit yield attributes of Cape gooseberry cv. Aligarh as affected by the use of different growth regulators. Agricultural Science Digest, 36: 138-141.
  • Mohamed, H.F., Hussien, M.N., Abd El-Hamed, K.E., Elwan, M.W., Abdel-Salam, M.M., 2021. Response of watermelon plants grafted onto different rootstocks to deficit irrigation. HortScience Journal of Suez Canal University, 10(1): 63-71.
  • Möller, I.M., Jensen, P.E., Hansson, A., 2007. Oxidative modifications to cellular components in plants. Annual Review of Plant Biology, 58(1): 459-481.
  • Nankishore, A., Farrell, A., 2016. The response of contrasting tomato genotypes to combined heat and drought stress. Journal of Plant Physiology, 1(202): 75-82.
  • Pourghayoumi, M., Bakhshi, D., Rahemi, M., Kamgar-Haghighi, A.A., Aalami, A., 2017. The physiological responses of various pomegranate cultivars to drought stress and recovery in order to screen for drought tolerance. Scientia Horticulturae, 217: 164-172.
  • Sarabi, B., Bolandnazar, S., Ghaderi, N., Ghashghaie, J., 2017. Genotypic differences in physiological and biochemical responses to salinity stress in melon (Cucumis melo L.) plants: Prospects for selection of salt-tolerant landraces. Plant Physiology and Biochemistry, 119: 294-311.
  • Shah, S.H., Islam, S., Mohammad, F., Siddiqui, M.H., 2023. Gibberellic acid: A versatile regulator of plant growth, development, and stress responses. Journal of Plant Growth Regulation, 42: 7352-7373.
  • Tuna, A.L., Kaya, C., Dikilitas, M., Higgs, D., 2008. The combined effects of gibberellic acid and salinity on some antioxidant enzyme activities, plant growth parameters and nutritional status in maize plants. Environmental and Experimental Botany, 62(1): 1-9.
  • Wu, Y., Gao, Q., Huang, S., Jia, S., 2019. Enhancing salt tolerance in melon by exogenous application of melatonin and Ca²⁺. Pakistan Journal of Botany, 51(3): 781-787.
  • Zhou, R., Yu, X., Ottosen, C.-O., Rosenqvist, E., Zhao, L., Wang, Y., Yu, W., Zhao, T., Wu, Z., 2017. Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress. BMC Plant Biology, 17: 24.

Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress

Year 2025, Volume: 12 Issue: 2, 155 - 161, 30.06.2025

Abstract

This study aimed to investigate the effects of drought stress on melon (Cucumis melo L.) and to evaluate the potential mitigating role of gibberellic acid (GA₃) application. In the experiment, a 10-day drought period was imposed by withholding irrigation, and GA₃ was applied at a concentration of 100 ppm. The results indicated that drought stress significantly suppressed plant growth. The SPAD value decreased by 26%, from 303.6 ± 8.9 in the control group to 224.1 ± 5.9 under drought conditions, while GA₃ application did not lead to a statistically significant improvement. Drought stress increased malondialdehyde levels from 3.6 to 7.9, whereas GA₃ treatment reduced it to 5.67, indicating a partial alleviation of oxidative stress. Root length decreased from 50 ± 8.1 cm in the control to 31 ± 5.09 cm under drought stress and was further reduced to 25 ± 6.03 cm with GA₃ application. Principal Coordinate Analysis revealed that GA₃ application did not completely mitigate drought stress but provided partial improvement. These findings highlight the detrimental effects of drought stress on melon and indicate that GA₃ alone may not be sufficient to alleviate drought-induced damage in melon cultivation.

References

  • Ahmed, C.B., Rouina, B.B., Boukhris, M., 2007. Effects of water deficit on olive trees cv. Chemlali under field conditions in arid region in Tunisia. Scientia Horticulturae, 113: 267-277.
  • Coşkun, Ö.F., 2023. The effect of grafting on morphological, physiological and molecular changes induced by drought stress in cucumber. Sustainability, 15(1): 875.
  • Coşkun, Ö.F., 2025. Association mapping for drought tolerance in watermelons (Citrullus lanatus L.). Horticulturae, 11(2): 193.
  • Doruk Kahraman, N., Okumuş, O., 2024. Role of gibberellic acid (GA₃) in improving salt stress tolerance of wheat (Triticum aestivum). Journal of Erciyes Agriculture and Animal Science, 7(2): 86-93.
  • Fang, Y.J., Xiong, L.Z., 2015. General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and Molecular Life Sciences, 72(4): 673-689.
  • Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A., 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212.
  • Gebril, S., Elsayed, H.M.A., 2025. Effect of drought stress on seedling morphological traits of four commercial hybrids of Egyptian watermelon. International Journal of Emerging Technologies, 16(1): 1-7.
  • Giordano, M., Petropoulos, S.A., Rouphael, Y., 2021. Response and defense mechanisms of vegetable crops against drought, heat, and salinity stress. Agriculture, 11(5): 463.
  • Grafton, R.Q., Daugbjerg, C., Qureshi, M.E., 2015. Towards food security by 2050. Food Security, 7: 179-183.
  • Imran, M., Latif Khan, A., Shahzad, R., Aaqil Khan, M., Bilal, S., Khan, A., Kang, S.-M., Lee, I.-J., 2021. Exogenous melatonin induces drought stress tolerance by promoting plant growth and antioxidant defense system of soybean plants. AoB Plants, 13(4): plab026.
  • Jaleel, C.A., Manivannan, P., Wahid, A., Farooq, M., Al-Juburi, H.J., Somasundaram, R., Panneerselvam, R., 2009. Drought stress in plants: A review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology, 11(1): 100-105.
  • Karahara, I., Horie, T., 2021. Functions and structure of roots and their contributions to salinity tolerance in plants. Breeding Science, 71(1): 89-108.
  • Kaur, G., Kaur, A., 2016. Plant growth and fruit yield attributes of Cape gooseberry cv. Aligarh as affected by the use of different growth regulators. Agricultural Science Digest, 36: 138-141.
  • Mohamed, H.F., Hussien, M.N., Abd El-Hamed, K.E., Elwan, M.W., Abdel-Salam, M.M., 2021. Response of watermelon plants grafted onto different rootstocks to deficit irrigation. HortScience Journal of Suez Canal University, 10(1): 63-71.
  • Möller, I.M., Jensen, P.E., Hansson, A., 2007. Oxidative modifications to cellular components in plants. Annual Review of Plant Biology, 58(1): 459-481.
  • Nankishore, A., Farrell, A., 2016. The response of contrasting tomato genotypes to combined heat and drought stress. Journal of Plant Physiology, 1(202): 75-82.
  • Pourghayoumi, M., Bakhshi, D., Rahemi, M., Kamgar-Haghighi, A.A., Aalami, A., 2017. The physiological responses of various pomegranate cultivars to drought stress and recovery in order to screen for drought tolerance. Scientia Horticulturae, 217: 164-172.
  • Sarabi, B., Bolandnazar, S., Ghaderi, N., Ghashghaie, J., 2017. Genotypic differences in physiological and biochemical responses to salinity stress in melon (Cucumis melo L.) plants: Prospects for selection of salt-tolerant landraces. Plant Physiology and Biochemistry, 119: 294-311.
  • Shah, S.H., Islam, S., Mohammad, F., Siddiqui, M.H., 2023. Gibberellic acid: A versatile regulator of plant growth, development, and stress responses. Journal of Plant Growth Regulation, 42: 7352-7373.
  • Tuna, A.L., Kaya, C., Dikilitas, M., Higgs, D., 2008. The combined effects of gibberellic acid and salinity on some antioxidant enzyme activities, plant growth parameters and nutritional status in maize plants. Environmental and Experimental Botany, 62(1): 1-9.
  • Wu, Y., Gao, Q., Huang, S., Jia, S., 2019. Enhancing salt tolerance in melon by exogenous application of melatonin and Ca²⁺. Pakistan Journal of Botany, 51(3): 781-787.
  • Zhou, R., Yu, X., Ottosen, C.-O., Rosenqvist, E., Zhao, L., Wang, Y., Yu, W., Zhao, T., Wu, Z., 2017. Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress. BMC Plant Biology, 17: 24.
There are 22 citations in total.

Details

Primary Language English
Subjects Vegetable Growing and Treatment
Journal Section Research Article
Authors

Seher Toprak 0000-0002-3459-9846

Ömer Faruk Coşkun 0000-0001-5398-5737

Publication Date June 30, 2025
Submission Date April 6, 2025
Acceptance Date June 25, 2025
Published in Issue Year 2025 Volume: 12 Issue: 2

Cite

APA Toprak, S., & Coşkun, Ö. F. (2025). Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress. Türkiye Tarımsal Araştırmalar Dergisi, 12(2), 155-161.
AMA Toprak S, Coşkun ÖF. Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress. TÜTAD. June 2025;12(2):155-161.
Chicago Toprak, Seher, and Ömer Faruk Coşkun. “Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis Melo L.) Under Drought Stress”. Türkiye Tarımsal Araştırmalar Dergisi 12, no. 2 (June 2025): 155-61.
EndNote Toprak S, Coşkun ÖF (June 1, 2025) Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress. Türkiye Tarımsal Araştırmalar Dergisi 12 2 155–161.
IEEE S. Toprak and Ö. F. Coşkun, “Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress”, TÜTAD, vol. 12, no. 2, pp. 155–161, 2025.
ISNAD Toprak, Seher - Coşkun, Ömer Faruk. “Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis Melo L.) Under Drought Stress”. Türkiye Tarımsal Araştırmalar Dergisi 12/2 (June 2025), 155-161.
JAMA Toprak S, Coşkun ÖF. Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress. TÜTAD. 2025;12:155–161.
MLA Toprak, Seher and Ömer Faruk Coşkun. “Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis Melo L.) Under Drought Stress”. Türkiye Tarımsal Araştırmalar Dergisi, vol. 12, no. 2, 2025, pp. 155-61.
Vancouver Toprak S, Coşkun ÖF. Determination of the Effects of Exogenous Gibberellic Acid on Melon (Cucumis melo L.) Under Drought Stress. TÜTAD. 2025;12(2):155-61.

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