Petrol kökenli ürünler
toprak ekosisteminde büyük tahribatlar yaratır. Bu durum, bu maddelerin düşük
biyolojik organik bileşik karışımlarının çoğu zaman potansiyel karsinojenik ve
mutajenik olarak tarif olmasından kaynaklanmaktadır. Biyolojik parçalanmaya
dayanıklı petrol kökenli hidrokarbonların toprakta birikmesi, enzim
aktiviteleri ve mikroorganizma varlığı gibi biyolojik parametrelerin
değişmesine sebep olmaktadır. Bu çalışmada, ham petrol ve atık mineral motor yağı
ile seçilen oranlarda (%0,5 ve %5) kirletilen topraklara organik kirleticinin
parçalanmasını kolaylaştırmak amacıyla arıtma çamuru ilave edilmiştir.
Hazırlanan örneklerle 12 aylık bir inkübasyon çalışması yürütülmüş olup seçilen
enzim aktiviteleri (üreaz, dehidrogeneaz, alkali fosfataz, β-glukosidaz)
analizlenmiştir. Çalışma sonuçlarına göre, arıtma çamuru ilavesi ham petrol ve
atık yağ ile kirlenmiş topraklarda enzim aktivitelerinin iyileşmesine katkıda
bulunmuştur. Üreaz, alkali fosfataz ve dehidrogenaz aktivitelerinin ham petrol
ve atık mineral yağ kirliliğinden olumsuz etkilendiği tespit edilmiş olup
β-glukosidaz aktivitesinde ise olumsuz bir etki gözlenmemiştir. Ham petrol ve
atık yağ ile kirlenmiş topraklar arasında bir kıyaslama yapıldığında kirletici
tipleri açısından enzim aktiviteleri üzerinde önemli bir farklılık tespit
edilmemiştir.
Petroleum products cause great devastation in soil ecosystems. This
results from the fact that these substances are mixtures of organic compounds
with a low bioavailability, often described as potentially carcinogenic and
mutagenic. As a result of the high potential of petroleum hydrocarbons to
accumulate in the soil environment, together with their resistance to
biodegradation, these compounds cause substantial changes in biological
parameters, such as enzymatic activity and abundance of microbes. The objective of this study was to
evaluate the effects of different types of hydrocarbon pollution (crude oil and
waste engine oil) on soil enzyme activities at the end of incubation period of
12 months. The possible use of wastewater sludge as a biostimulating agent in
petroleum-contaminated soils was also evaluated. Enhanced enzyme activity
levels in contaminated soils indicated that crude oil and waste engine oil
appeared to stimulate microbial growth and enzyme activity in the soil
environment. Results showed that hydrocarbon contamination inhibited enzymatic
activities (except β-glucosidase) in all the amended soil samples. The effect of wastewater sludge amendment on
soil enzymes in polluted soil appears to depend on contaminant dose and the
origin of the petroleum product.
Subjects | Engineering |
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Journal Section | Research Articles |
Authors | |
Publication Date | April 10, 2017 |
Submission Date | August 29, 2016 |
Acceptance Date | February 23, 2017 |
Published in Issue | Year 2017 Volume: 22 Issue: 1 |
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