Hidrojen Sulfid Donörü Sodyum Hidrojen Sülfür’ün In Vitro Yara İyileşmesi Üzerine Etkisinin Araştırılması

Yıl 2023, , 397 - 403, 31.12.2023

Aysun Özbay Önal , Mustafa Önal

https://doi.org/10.32708/uutfd.1390621

Öz

Yara iyileşmesi sürecinde büyüme faktörlerinin hücre proliferasyonu, hücre farklılaşması ve hücre ölümü gibi çeşitli biyolojik aktiviteleri mevcuttur. Gazotransmitterler yara iyileşme sürecinde etkinliği gösterilmiş sinyal molekülleridir. Gazotransmitter ailesinin bir üyesi olan H2S’in in vivo çalışmalarda yara iyileşmesinde düzenleyici bir molekül olarak görev aldığı bildirilmektedir. H2S’in yara iyileşme sürecinde çeşitli sinyal yolaklarının aktivasyonu aracılığıyla indirekt etkisinin olduğu görülmektedir. Bu çalışmada H2S’in indirekt etkisinden ziyade direkt etkisinin olup olmadığı araştırılmıştır. Bu bağlamda H2S donörü olan NaHS’in in vitro yara iyileşmesinde fonksiyon gösteren çeşitli genlerin ekspresyon düzeylerine olan etkisi değerlendirilmiştir. İmmortalize insan keratinosit hücreleri 50μg, 25μg, 10μg, 5μg, 1μg konsantrasyonda NaHS ile 24, 48 ve 72 saat boyunca inkübe edilmiş ve MTS analizi ile hücre canlığı belirlenmiştir. TGF-β1, TGF-β3, VEGF ve K17 gen ifadelerindeki değişimler qRT-PCR yöntemiyle belirlenerek elde edilen veriler ΔΔCT metodu ile hesaplanmıştır. Hücre canlılığı açısından farklı konsantrasyonlarda uygulanan NaHS’in 1μg’lık dozunun toksik olmayan doz olduğu belirlenmiştir. NaHS uygulamasının K17 mRNA ekspresyonunu anlamlı düzeyde arttırırken TGF-β1, TGF-β3 ve VEGF ekspresyonunda anlamlı değişikliğe yol açmadığı saptanmıştır. NaHS’in in vitro yara iyileşmesi üzerine direkt etkisinin olmadığı sonucuna varılmıştır.

Anahtar Kelimeler

H2S, NaHS, yara iyileşmesi

Etik Beyan

Çalışmamızda ticari olarak satın alınan insan hücre hattı kullanılmıştır. Etik kurul onayına gerek yoktur.

Investigation of the Effect of Hydrogen Sulfide Donor Sodium Hydrogen Sulfide on In Vitro Wound Healing

Öz

In the wound healing process, various biological activities such as cell proliferation, cell differentiation, and cell death are mediated by growth factors. Gasotransmitters are signaling molecules that have been shown to be effective in the wound healing process. H2S, a member of the gasotransmitter family, is reported to function as a regulatory molecule in wound healing in in vivo studies. The indirect effect of H2S in the wound healing process is observed through the activation of various signaling pathways. This study investigates whether H2S has a direct effect rather than an indirect effect. In this context, the impact of NaHS, an H2S donor, on the expression levels of various genes involved in in vitro wound healing has been evaluated. Immortalized human keratinocyte cells were incubated with NaHS at concentrations of 50 μg, 25 μg, 10 μg, 5 μg, and 1μg for 24, 48, and 72 hours and cell viability was determined by MTS analysis. Changes in the expression of TGF-β1, TGF-β3, VEGF and K17 genes were determined using qRT-PCR, and the data were calculated using the ΔΔCT method. In terms of cell viability, it was determined that the 1μg dose of NaHS is a non-toxic dose. While NaHS application significantly increased K17 mRNA expression, it didn’t cause significant changes in TGF-β1, TGF-β3 and VEGF expression. It was concluded that NaHS doesn’t have a direct effect on in vitro wound healing.

Anahtar Kelimeler

H2S, NaHS, wound healing

Kaynakça

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