Evaluation of the Morphological Effects of Diabetes on Uterine Tissue and Mucin-1 Expression

Abstract

Diabetes mellitus (DM), is considered one of the most important health problems in today's world. The emergence of free radicals in DM causes oxidative stress. In our study, after creating an experimental DM model with streptozotocin (STZ) on rats, we aimed to determine whether DM has any effect on the uterine tissue of the animals. An 8-10 week old Wistar Albino adult female rat weighing approximately 250-300 grams was used in the study. They were divided into two groups: control and diabetic. According to the estrous cycle of animals; Estrus periods were determined and uterine tissues were taken during this period. STZ applied to rats at concentrations of 60 mg/kg showed that intraperitoneal (IP) were erased. Two days after STZ administration, blood glucose characteristics were examined from the tail vein. Rats with a value greater than 200-300 mg/dL were considered diabetic. After 30 days, the uterine tissues were removed. 3 μm thick sections were taken from tissue samples. In the uterine tissue taken, hematoxylin and eosin (H&E) staining methods were used for morphology, and Picro-sirius red staining methods were used to show collagen fibers. Mucin-1 (MUC1) antibody was used for immunofluorescence staining. Histopathological and morphometric changes in the endometrium region of the uterine tissue in the diabetic were observed. In our study, it was determined that DM reduces the uterine endometrium collagen fiber density and has negative effects on the glands. As a result, diabetes can lead to tissue concentration and the negative effects on gland structures will continue.

Keywords

• STZ
• Uterus
• Endometrium
• Collagen
• Muc-1

Diyabetin Uterus Dokusu Üzerinde Morfolojik Etkilerinin ve Mucin-1 Ekspresyonunun Değerlendirilmesi

Abstract

Diyabet günümüz dünyasında en önemli sağlık sorunlarından birisi olarak kabul edilmektedir. Diyabette serbest radikallerin ortaya çıkması oksidatif strese neden olur. Çalışmamızda, ratlar üzerinde streptozotosin (STZ) ile deneysel diyabet modeli oluşturulması sonrasında, diyabetin, hayvanların uterus dokusu üzerinde herhangi bir etkisinin olup olmadığının araştırılması amaçlanmıştır. Çalışmada ağırlıkları yaklaşık 250-300 gram ağırlığında 8-10 haftalık Wistar Albino cinsi erişkin dişi rat kullanıldı. Kontrol ve diyabet olmak üzere iki gruba ayrıldı. Hayvanlar östrus siklusuna göre; östrüs dönemleri belirlenerek bu dönemde uterus dokuları alındı. Sıçanlara 60 mg/kg derişimlerde hazırlanan STZ, intraperitonal (IP) yoldan enjekte edildi. STZ verildikten iki gün sonra kuyruk veninden kan glukoz seviyesine bakıldı. Değeri 200-300 mg/dL’den büyük olan sıçanlar diyabet kabul edildi. 30 gün sonra uterus dokuları çıkarıldı. Doku örneklerinden 3 μm kalınlığında kesitler alındı. Alınan uterus dokusunda, morfoloji için (H&E), kollajen lifleri gösterebilmek için ise Picro-sirius red boyama yöntemleri kullanıldı. İmmünfloresan boyama için ise Mucin-1 (MUC1) antikoru kullanıldı. Diyabet grubunda yer alan uterus dokusunun endometrium bölgesindeki histopatolojik ve morfometrik değişiklikler izlendi. Yapılan çalışmada, diyabetin uterus endometrium kollajen lif yoğunluğunu azalttığı ve bezler üzerinde de olumsuz etkilerinin olduğu belirlendi. Sonuç olarak diyabetin doku bozulmasına yol açabileceği ve bez yapıları üzerinde olumsuz etkilerinin olabileceği düşünülebilir.

Keywords

• STZ
• Uterus
• Endometriyum
• Kollajen
• Mucin-1

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