RHOB Ekspresyonun Nöroblastomda Moleküler Risk Sınıfları ve Prognoz ile İlişkisinin Değerlendirilmesi

Abstract

Nöroblastom, gelişmekte olan sinir sisteminden köken alan ve çocukluk çağının en sık görülen solid tümörüdür. Klinik seyri lokalize veya spontane gerileme gösterebilen hastalıktan, yoğun ve multimodal tedavi rejimlerine dirençli metastatik hastalığa değişen heterojen bir davranış göstermektedir. Ras Homolog Ailesi Üyesi B (RhoB), Rho GTPaz ailesinin bir üyesidir ve sitoiskeletet organizasyonunda, reseptör ve sinyal proteinlerinin iletiminde, DNA hasar tamirinde ve apoptozda rol oynar. RhoB ekspresyonu farklı erişkin kanser türlerinde incelenmiş ve farklı kanser türlerinde iyi veya kötü prognozla ilişkili olduğu saptanmıştır. Ne var ki, RhoB ekspresyonu aday bir prognostik biyobelirteç olarak pediatrik kanserlerde daha önce çalışılmamıştır. Yapılan çalışmamızda, RhoB protein ekspresyonu nöroblastom hastalarında immün histokimyasal boyama yöntemiyle incelenmiştir. RhoB ekspresyonu ile düşük, orta ve yüksek risk gruplarındaki nöroblastom hastalarının genel sağ kalımı ve olaysız sağ kalımları karşılaştırılmış ve RhoB ekspresyonu ile hastaların klinikopatolojik özellikleri Spearman’s korelasyon analizi ile incelenmiştir. Her ne kadar çalışmamızdaki hasta örneklem sayısı az olsa da çalışmamızda RhoB ekspresyonunun NB hastaları için iyi prognostik bir biyobelirteç olabileceği ortaya konmuştur.

Keywords

• Nöroblastom
• Çocukluk çağı kanserleri
• Biyobelirteç
• Sağ kalım
• Prognostik faktör

Evaluation of the Association of RHOB Expression with Molecular Risk Classes and Prognosis in Neuroblastoma

Abstract

Neuroblastoma is the most common solid tumor of childhood originating from the developing nervous system. It shows a heterogeneous behavior with a clinical course ranging from localized or spontaneously regressive disease to metastatic disease resistant to intensive and multimodal treatment regimens. Ras Homolog Family Member B (RhoB) is a member of the Rho GTPase family and plays a role in cytoskeleton organization, receptor and signaling protein transmission, DNA damage repair and apoptosis. RhoB expression has been studied in different adult cancer types and has been found to be associated with good or poor prognosis in different cancer types. However, RhoB expression has not been studied as a candidate prognostic biomarker in pediatric cancers before. In our study, RhoB protein expression was examined in neuroblastoma patients by immunohistochemical methods. Overall survival and event-free survival of neuroblastoma patients in low, intermediate and high-risk groups were compared with RhoB expression and clinicopathological features of patients were examined with Spearman's correlation analysis. Although the number of patients in our study was small, our study revealed that RhoB expression may be a good prognostic biomarker for NB patients.

Keywords

• Neuroblastoma
• Pediatric cancers
• Biomarker
• Survival
• Prognostic factor

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