Böbrek Dokusunda Akuaporinler Üzerine Bakır Sülfat Etkisi

Abstract

Bakır (Cu), çoğu enzimin kofaktörü ve temel bir eser elementtir. Ancak, yüksek düzeyde Cu maruziyeti çeşitli dokularda toksik etkilere neden olur. Akuaporinler (AQP), sıvı taşınmasında, özellikle böbrek dokularında önemli bir rol oynayan integral membran proteinleridir. Çalışmamızda, yüksek doz bakır sülfat (CuSO4) uygulamasının ardından dişi ve erkek sıçanların böbreklerinde AQP-1, -2 ve -3 ekspresyonunu değerlendirmeyi amaçladık. İlk olarak, kontrol grubuna (n=10) oral gavaj yoluyla salin verilirken, deney grubuna (CuSO4) (n=10) 14 gün boyunca oral gavaj yoluyla 100 mg/kg/gün CuSO4 verildi. Daha sonra kontrol ve deney gruplarının böbrek dokularında AQP-1, -2 ve -3'ün histopatolojik, PAS boyama ve immünohistokimyasal değerlendirmeleri yapıldı. CuSO4, böbreklerde tübüler vakuolizasyona neden oldu. CuSO4 uygulaması, AQP-1 ve AQP-3 düzeylerini artırırken AQP-2 düzeylerini düşürdü. Bu bulgular, CuSO4'ün neden olduğu tübüler vakuolizasyon ve dejenerasyon nedeniyle AQP-1 ve AQP-3 düzeylerini yeniden modüle etmiş olabileceğini düşündürmektedir; ancak CuSO4'ün dişilerdeki östrojen düzeyleri üzerindeki etkisi ve AQP-2 aracılığıyla vazopressin etkisi ayrıntılı olarak araştırılmalıdır.

Keywords

• Akuaporinler
• Bakır Sülfat
• Böbrek
• Rat

The Effect of Copper Sulfate on Aquaporins in Kidney Tissue

Abstract

Copper (Cu) is a cofactor for most enzymes and an essential trace element. However, elevated levels of Cu exposure cause toxic effects in various tissues. Aquaporins (AQP) are integral membrane proteins that play a key role in fluid transport, particularly in kidney tissues. In our study, we aimed to evaluate the expression of AQP-1, -2, and -3 in the kidneys of female and male rats following the administration of high-dose copper sulfate (CuSO4). First, the control group (n=10) received saline via oral gavage, while the experimental group (CuSO4) (n=10) received 100 mg/kg/day via oral gavage for 14 days. Histopathological, PAS staining, and immunohistochemical evaluation of AQP-1, -2, and -3 were then performed on kidney tissues from the control and experimental groups. CuSO4 caused tubular vacuolization in the kidneys. CuSO4 administration decreased AQP-2 levels while increasing AQP-1 and AQP-3 levels. These findings suggest that CuSO4 may have re-modulated AQP-1 and AQP-3 levels due to the tubular vacuolization and degeneration it caused; however, the effect of CuSO4 on estrogen levels in females and the vasopressin effect via AQP-2 need to be investigated in detail.

Keywords

• Aquaporins
• Cupper sulfate
• Kidney
• Rat

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