Research Article

Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality

Volume: 52 March 26, 2026
TR EN

Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality

Abstract

This study aimed to determine the effects of adding cumulus cell extracellular matrix (CCECM) to the culture medium used in sperm preparation techniques for assisted reproductive treatments (ART) on routine sperm parameters, fertilization capacity, and sperm DNA fragmentation, and to compare sperm separation protocols. Ejaculate samples from 30 normozoospermic male patients and cumulus–oocyte complexes collected from their spouses were included. Each semen sample was divided into five equal volumes and allocated to five groups: control, density gradient (DG), density gradient plus cumulus cell extracellular matrix (DG+K), swim-up (SU), and swim-up plus cumulus cell extracellular matrix (SU+K). The results showed that sperm concentration was markedly lower in all experimental groups than in the control group, with the decrease reaching statistical significance, particularly in the swim-up group. Motility was highest in the SU+K group and was also significantly increased in the DG+K and SU groups. In contrast, the TPMSS value decreased significantly in the SU and SU+K groups. No marked differences were observed among the groups in terms of morphology. Viability increased notably in the CCECM-supplemented groups, and this increase remained significant across all experimental groups compared with the control group. ARIC scores, reflecting acrosomal reaction capacity, were significantly higher in the DG+K and SU+K groups. Moreover, DFI values, indicating DNA damage, were significantly lower in these two groups, suggesting that CCECM supplementation may help preserve sperm DNA integrity. Adding CCECM to the culture medium used in sperm washing improved sperm quality and efficiency, particularly with the swim-up method. We believe this approach may be a feasible option for treating male infertility within assisted reproductive technologies (ART).

Keywords

Supporting Institution

Bursa Uludağ University Scientific Research Projects Coordination Unit (BAP)

Project Number

TYL-2025-2129

Ethical Statement

The clinical research ethics committee of Bursa Uludag University Faculty of Medicine, Approval Date: 08.11.2023, Decision No: 2023-21/32.

Thanks

This study was conducted within the framework of the Bursa Uludağ University Scientific Research Projects Coordination Unit (BAP) project TYL-2025-2129, and we gratefully acknowledge the support of the BAP Coordination Unit.

References

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  7. 7. Parmegiani L, Cognigni GE, Bernardi S, Troilo E, Ciampaglia W, Filicori M. "Physiologic ICSI": hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil Steril. 2010;93(2):598-604.
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Details

Primary Language

English

Subjects

Reproductive Medicine (Other)

Journal Section

Research Article

Publication Date

March 26, 2026

Submission Date

February 25, 2026

Acceptance Date

March 24, 2026

Published in Issue

Year 2026 Volume: 52

APA
Aghayeva, S., Durdu, T. Y., Çakır, C., Aslan, K., Kasapoğlu, I., & Gök Yurtseven, D. (2026). Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality. Journal of Uludağ University Medical Faculty, 52, 1897295. https://doi.org/10.32708/uutfd.1897295
AMA
1.Aghayeva S, Durdu TY, Çakır C, Aslan K, Kasapoğlu I, Gök Yurtseven D. Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality. Journal of Uludağ University Medical Faculty. 2026;52:1897295. doi:10.32708/uutfd.1897295
Chicago
Aghayeva, Sabina, Talha Yasin Durdu, Cihan Çakır, Kiper Aslan, Işıl Kasapoğlu, and Duygu Gök Yurtseven. 2026. “Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality”. Journal of Uludağ University Medical Faculty 52 (March): 1897295. https://doi.org/10.32708/uutfd.1897295.
EndNote
Aghayeva S, Durdu TY, Çakır C, Aslan K, Kasapoğlu I, Gök Yurtseven D (March 1, 2026) Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality. Journal of Uludağ University Medical Faculty 52 1897295.
IEEE
[1]S. Aghayeva, T. Y. Durdu, C. Çakır, K. Aslan, I. Kasapoğlu, and D. Gök Yurtseven, “Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality”, Journal of Uludağ University Medical Faculty, vol. 52, p. 1897295, Mar. 2026, doi: 10.32708/uutfd.1897295.
ISNAD
Aghayeva, Sabina - Durdu, Talha Yasin - Çakır, Cihan - Aslan, Kiper - Kasapoğlu, Işıl - Gök Yurtseven, Duygu. “Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality”. Journal of Uludağ University Medical Faculty 52 (March 1, 2026): 1897295. https://doi.org/10.32708/uutfd.1897295.
JAMA
1.Aghayeva S, Durdu TY, Çakır C, Aslan K, Kasapoğlu I, Gök Yurtseven D. Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality. Journal of Uludağ University Medical Faculty. 2026;52:1897295.
MLA
Aghayeva, Sabina, et al. “Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality”. Journal of Uludağ University Medical Faculty, vol. 52, Mar. 2026, p. 1897295, doi:10.32708/uutfd.1897295.
Vancouver
1.Sabina Aghayeva, Talha Yasin Durdu, Cihan Çakır, Kiper Aslan, Işıl Kasapoğlu, Duygu Gök Yurtseven. Effects of Cumulus Cell Extracellular Matrix–Assisted Sperm Preparation Protocols on In Vitro Sperm Quality. Journal of Uludağ University Medical Faculty. 2026 Mar. 1;52:1897295. doi:10.32708/uutfd.1897295

ISSN: 1300-414X, e-ISSN: 2645-9027

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