Research Article

Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury

Volume: 52 September 10, 2026
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Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury

Abstract

The aim of this study was to evaluate the early histopathological and inflammatory effects of acute insulin-induced hypoglycemia in liver and kidney tissues. Acute hypoglycemia was experimentally induced by insulin administration, and liver and kidney samples were analyzed histologically. Tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and nuclear factor kappa B (NF-κB) protein expression were assessed by immunohistochemistry, while TNF-α and IL-6 gene expression were evaluated using reverse transcription quantitative polymerase chain reaction. Acute hypoglycemia induced early structural alterations in both organs, with more pronounced and progressive changes observed in the kidney. Renal injury included tubular epithelial degeneration, tubular dilatation, and glomerular alterations, whereas hepatic changes were milder and mainly characterized by sinusoidal dilatation and hyperemia. No significant differences were observed in TNF-α, IL-6, or NF-κB protein expression. However, renal TNF-α and IL-6 messenger RNA levels showed transient alterations over time. In conclusion, acute insulin-induced hypoglycemia leads to early structural injury in liver and kidney tissues, with greater susceptibility observed in the kidney. These changes occur in the absence of significant inflammatory protein activation, suggesting that early tissue injury is primarily driven by metabolic stress rather than overt inflammatory signaling, although transient transcriptional responses may precede detectable protein-level changes.

Keywords

Ethical Statement

Approving Committee: Erciyes University Animal Experiments Local Ethics Committee Approval Date: 03.12.2025 Decision No: 25/273

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Publication Date

September 10, 2026

Submission Date

June 6, 2026

Acceptance Date

August 11, 2026

Published in Issue

Year 2026 Volume: 52

APA
Mehmetbeyoğlu Duman, E., Sancak, A. S., Abusalim, M. R. S., Kaçamak, P., Aktaş, S., Doğanyiğit, Z., & Taheri, S. (2026). Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury. Journal of Uludağ University Medical Faculty, 52. https://doi.org/10.32708/uutfd.1964679
AMA
1.Mehmetbeyoğlu Duman E, Sancak AS, Abusalim MRS, et al. Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury. Journal of Uludağ University Medical Faculty. 2026;52. doi:10.32708/uutfd.1964679
Chicago
Mehmetbeyoğlu Duman, Ecmel, Amine Sudenur Sancak, Mai R S Abusalim, et al. 2026. “Insulin-Induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers With Liver and Kidney Injury”. Journal of Uludağ University Medical Faculty 52 (September). https://doi.org/10.32708/uutfd.1964679.
EndNote
Mehmetbeyoğlu Duman E, Sancak AS, Abusalim MRS, Kaçamak P, Aktaş S, Doğanyiğit Z, Taheri S (September 1, 2026) Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury. Journal of Uludağ University Medical Faculty 52
IEEE
[1]E. Mehmetbeyoğlu Duman et al., “Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury”, Journal of Uludağ University Medical Faculty, vol. 52, Sept. 2026, doi: 10.32708/uutfd.1964679.
ISNAD
Mehmetbeyoğlu Duman, Ecmel - Sancak, Amine Sudenur - Abusalim, Mai R S - Kaçamak, Pınar - Aktaş, Sümeyye - Doğanyiğit, Züleyha - Taheri, Serpil. “Insulin-Induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers With Liver and Kidney Injury”. Journal of Uludağ University Medical Faculty 52 (September 1, 2026). https://doi.org/10.32708/uutfd.1964679.
JAMA
1.Mehmetbeyoğlu Duman E, Sancak AS, Abusalim MRS, Kaçamak P, Aktaş S, Doğanyiğit Z, Taheri S. Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury. Journal of Uludağ University Medical Faculty. 2026;52. doi:10.32708/uutfd.1964679.
MLA
Mehmetbeyoğlu Duman, Ecmel, et al. “Insulin-Induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers With Liver and Kidney Injury”. Journal of Uludağ University Medical Faculty, vol. 52, Sept. 2026, doi:10.32708/uutfd.1964679.
Vancouver
1.Ecmel Mehmetbeyoğlu Duman, Amine Sudenur Sancak, Mai R S Abusalim, Pınar Kaçamak, Sümeyye Aktaş, Züleyha Doğanyiğit, Serpil Taheri. Insulin-induced Hypoglycemia in a Mouse Model: Association of Inflammatory Markers with Liver and Kidney Injury. Journal of Uludağ University Medical Faculty. 2026 Sep. 1;52. doi:10.32708/uutfd.1964679

ISSN: 1300-414X, e-ISSN: 2645-9027

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