In Vitro Study of the Effect of 1.0% Sodium Hyaluronate on Bacterial Strains and Antibiotics

Year 2025, Volume: 51 Issue: 2, 209 - 215, 28.08.2025

Nazmiye Ülkü Tüzemen , Esin Söğütlü Sarı , Tuncay Topaç , Kadir Efe , Güven Özkaya , Cüneyt Özakın , Mehmet Baykara

https://doi.org/10.32708/uutfd.1641884

Abstract

During anterior segment eye surgeries, ophthalmic viscosurgical devices (OVDs), primarily containing hyaluronic acid, protect the corneal endothelium and help maintain the anterior chamber with reduced trauma. We aimed to investigate the interaction of sodium hyaluronate 1.0% with Gram-positive and Gram-negative bacteria, and its interactions with prophylactic intracameral antibiotics moxifloxacin and cefuroxime. Four quality control bacterial strains were used in vitro to conduct five experiments using either cefuroxime or moxifloxacin. Our experiment included five stages: 1. Testing the ability of OVD to retain bacteria before antibiotic exposure. 2. Examining antibiotic-bacteria interactions in the presence of OVD. 3. Simulating aqueous humor circulation. 4. Evaluating the effect of OVD residuals on antibiotic retention. 5. Analyzing the interaction of bacteria and antibiotics within the OVD. Results showed a significant decrease in bacterial counts (p<0.001) between the initial stage and subsequent groups, with moxifloxacin consistently demonstrating lower bacterial counts and greater effectiveness compared to cefuroxime (p<0.001). Further in vivo studies are recommended to validate these results.

Keywords

Sodium hyaluronate 1.0% , cefuroxime , moxifloxacin , ophthalmic viscosurgical devices

%1.0 Sodyum Hyaluronatın Bakteriler ve Antibiyotikler Üzerindeki Etkisinin İn Vitro Çalışması

Abstract

Ön segment göz ameliyatları sırasında, öncelikli olarak hyaluronik asit içeren oftalmik viskocerrahi cihazlar (OVD'ler), kornea endotelini korur ve ön bölmenin daha az travma ile korunmasına yardımcı olur. Çalışmamızda %1.0 Sodyum hyaluronat içeren OVD’nin Gram pozitif ve Gram negatif bakterilerle etkileşimini ve profilaktik olarak kullanılan intrakamaral moksifloksasin ve sefuroksim ile etkileşimlerini araştırmayı amaçladık. Sefuroksim veya moksifloksasin kullanılarak beş deney yürütmek için dört kalite kontrol bakteri suşu in vitro kullanıldı. Deneyimiz beş aşamadan oluşmaktadır; 1. Antibiyotik maruziyetinden önce OVD'nin bakterileri tutma yeteneğini test etme, 2. OVD varlığında antibiyotik-bakteri etkileşimlerini inceleme, 3. Humor aköz dolaşımını simüle etme, 4. OVD kalıntılarının antibiyotik tutması üzerindeki etkisini değerlendirme, 5. OVD içindeki bakteri ve antibiyotik etkileşimini analiz etme. İlk deney grubundaki bakteri sayısı ve sonraki aşamalar arasındaki bakteri sayılarında önemli bir azalma olduğu (p<0,001) saptandı. Moksifloksasin, sefuroksim ile karşılaştırıldığında daha fazla etkinlik gösterdi (p<0,001). Çalışmamız invitro bir çalışma olup, sonuçları doğrulamak için daha fazla in vivo çalışma önerilmektedir.

Keywords

sefuroksim , moksifloksasin , oftalmik viskocerrahi cihaz , %1 Sodyum hyaluronat

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