Öz
Bu çalış
mada, ravent (Rheum rhabarbarum L.)
saplarının metanol, etil asetat ve heksan ekstraktlarının antioksidan
aktiv itele ri farklı in vitro model
sistemle ri ku llan ıla rak değerlendirilmiştir.
Ravent saplarının polar çözücüdeki ekstraktlarının
serbest radikal süpürme aktiv
itele ri, 2,2'-a zino-b is (3- etilbenzotia
zo lin-6-sülfon ik asit),
hidro ksil ve 1,1-
difenil-2-p ikrilh idra
zil radika lle ri kullanılara k
ayrı ayrı ölçülmüştür. Her bir ekstraktın
demir iyonlarını şelatlama kabiliyetleri ve toplam
antioksidan kapasiteleri araştırılmıştır. Buna ek olarak, ravent sap
ekstraktlarının toplam fenolik, flavonoid ve flavonol içerikleri be lirlen
miştir. Metanol ekstresi, en yüksek
1,1-difenil-2-pikrilhidrazil radikalini temizleme
aktivitesini göstermişte olup, ardından etilasetat
ve
hekzan ekstreleri
ve
son olarak
da
bütil hidroksi
toluen yer
almaktaydı. Etil asetat ekstraktı,
en yüksek 2,2'-azino-bis (3-etilbenzotiazolin-6-sülfonik asit) radika
l temizle me aktiv itesini gösterirken (IC50
değeri 59,7±1,1 μg/mL), bunu bütil hidroksi toluen ve metanol
ekstraktı takip etmekteydi (sırasıyla
53.1±2.2 ve 47,9±1,3
μg/mL), ancak hidroksil
radikalini temizleme aktivitesini en fazla metanol
ekstraktı göstermekteydi (IC50
değeri
75.1±2.9 μg/mL). Toplam antioksidan
aktivite, en fazla metanol ve etilasetat ekstraktlarında bulunmuştur
(1 mg ekstrakta sırasıyla 284,1±11,6 ve 237,6±10 μg askorbik asit eşdeğeri).
100 μg/ mL konsantrasyonundaki metanol ve etilasetat ekstraktları, demir
iyonlarına karşı en yüksek şelatlama akt ivitesini göstermişlerd ir (sırasıy la
% 96,3±2,2 ve % 89,8±1,5). Ravent sap ekstraktları, kullanılan çözücüye ve
konsantrasyona bağlı olarak oldukça belirgin bir antioksidan aktivite
göstermiştir. Ravent sap ekstraktlarının güçlü bir antioksidan
potansiyel göstermesi, Rheum rhabarbarum L.’nin
ümit verici bir bitki olduğunu düşündürmektedir.
Anahtar Kelimeler
Kaynakça
- Agarwal SK, Singh SS, Lakshmi, V, Verma S, Kumar S. Chemistry and pharmacology of rhubarb Rheum species-A review. JSIR 2001;60(1):1-9.
- Al-Dabbas M, Kitahara K, Suganuma T, Hashimoto F, Tadera K. Antioxidant and α-amylase inhibitory compounds from aerial parts of Varthemia iphionoides. Biosci Biotechnol Biochem 2006;70(9):2178-84.
- Broadhurst CL, Polansky MM, Anderson RA. Insulin-like activity of culinary and medicinal plant aqueous extracts in vitro. J Agric Food Chem 2000;48(3):849-52.
- Clementi EM, Misiti F. Potential Health Benefits of Rhubarb. In: Watson RR, Preedy VR (Editors). Bioactive Foods in Promoting Health. 1st Edition, San Diego: Academic Press, 2010:407-423.
- Dregus M, Engel KH. Volatile constituents of uncooked rhubarb (Rheum rhabarbarum L.) stalks. J Agric Food Chem 2003;51(22):6530-6.
- Duh P, Yen G. Antioxidative activity of three water extracts. Food Chem 1997;60(4):639-45. Elmastaş M, Gülçin D, Beydemir Ş, Küfrevioğlu ÖD, Aboul-Enein HY. A study on the invitro antioxidant activity of juniper (Juniperus communis L.) seeds extracts. Anal Lett 2006;39(1):47-65.
- Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root; their relative astringency and radical scavenging effects. Chem Pharm Bull 1988;36(6):2090-7.
- Jang WH, Hsu WH, Hengel MJ, Shibamoto T. Antioxidant activity of rhubarb (Rheum rhabarbarum L.) extract and its main component emodin. Nat Prod Chem Res 2018;6(3):316-9.
- Kumar K, Ganesan K, Rao P. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty-An edible seaweed. Food Chem 2008;107(1):289-95.
- Lin CC, Wu CI, Lin TC, Sheu SJ. Determination of 19 rhubarb constituents by high-performance liquid chromatography- ultraviolet-mass spectrometry. J Sep Sci 2006;29(17):2584-93.
- Lu Y, Foo Y. Antioxidant radical scavenging activities polyphenols from apple pomace. Food Chem 2000;68(1):81-5.
- Matsuda H, Morikawa T, Toguchida I, Park JY, Harima S, Yoshikawa M. Antioxidant constituents from rhubarb: structural requirements of stilbenes for the activity and structures of two new anthraquinone glucosides. Bioorg Med Chem 2001;9(1):41-50. Menaga D, Rajkumar S, Ayyasami P. Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. Int J Pharm Pharm Sci 2013;5(4):601-6. Miliauskas G, Venskutonis PR, Van-beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85(2):231-7. Oluyemi K, Okwuonu U, Baxter D, Oyesola T. Toxic effects of methanolic extract of Aspilia africana leaf on the estrous cycle and uterine tissues of Wistar rats. Int J Morphol 2007;25(3):609-14.
- Ozgen M, Resse R, Tulio A, Scheerens J, Miller R. Modified 2,2`- azino-bis-3-ethylbezthiazoline-6 sulphonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2`-diphenyl-1-picrylhydrazyl (DPPH) methods. J Agric Food Chem 2006;54(4):1151-7.
- Öztürk M, Aydoğmuş-Öztürk F, Duru ME, Topçu G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chem 2007;103(2):623-30.
- Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269(2):337- 41.
- Umamaheswari M, Chatterjee T. In vitro antioxidant activities of the fractions of Coccinnia grandis L. leaf extract. Afr J Trad Compl Altern Med 2008;5(1):61-73.
- Wang J, Zhao Y, Xiao X, Li H, Zhao H, Zhang P, Jin C. Assessment of the renal protection and hepatotoxicity of rhubarb extract in rats. J Ethnopharmacol 2009;124(1):18-25.
Öz
In this study, the antioxidant
activities of methanol, ethyl acetate and hexane extracts from stalks of
Rhubarb (Rheum rhabarbarum L.) were evaluated using different model systems
ivitro. Free radical scavenging activities were assessed by measuring the
scavenging activities of stalks different polar e xt racts separately using
2,2'-azino-bis (3-
ethylbenzothiazoline-6-sulphonic acid, hydro xyl and 1,1-diphenyl-2 picrylhydrazyl radicals. Effect
of extracts on ferrous ions chelating ability and total antioxidant
capacity were also investigated for
each extract. In addition, total
phenolic, flavonoid and flavonol content of rhubarb stalks ext racts were
determined. The stalk methanol e xtract showed significantly the highest
1,1-diphenyl-2- picrylhydrazyl radical scavenging activity, followed by stalks
ethylacetate extract, stalks hexane extract and
lastly, butylated hydroxytoluene. Ethylacetate extract showed the highest
2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical
scavenging activity with
IC50 value
of 59.7±1.1 μg/mL, fo llowed by
butylated hydroxytoluene and methanol e xt ract with IC50 value of
53.1±2.2 and 47.9±1.3 μg/ mL, respectively, whereas the highest hydroxyl
radical inhibition activity was found for methanol extracts with IC50
values of 75.1±2.9 μg/mL. The total antioxidant
activity was the highest in methanol and ethylacetate
extracts (284.1±11.6 and 237.6±10.0
μg ascorbic acid
equivalent for 1 mg extract, respectively). Methanol
and ethylacetate extracts at concentration
of 100 μg/ mL showed the highest chelating activity against ferrous ions
(96.3±2.2% and 89.8±1.5%, respectively). All rhubarb stalk extracts showed
pronounced antioxidant activities in a dose-dependent manner and the effects
depend strongly on the solvent used for extraction. The results showed that
extracts of the stalk of rhubarb exhibit antioxidant potential suggesting that
Rheum rhabarbarum L. is a promising plant.
Anahtar Kelimeler
Kaynakça
- Agarwal SK, Singh SS, Lakshmi, V, Verma S, Kumar S. Chemistry and pharmacology of rhubarb Rheum species-A review. JSIR 2001;60(1):1-9.
- Al-Dabbas M, Kitahara K, Suganuma T, Hashimoto F, Tadera K. Antioxidant and α-amylase inhibitory compounds from aerial parts of Varthemia iphionoides. Biosci Biotechnol Biochem 2006;70(9):2178-84.
- Broadhurst CL, Polansky MM, Anderson RA. Insulin-like activity of culinary and medicinal plant aqueous extracts in vitro. J Agric Food Chem 2000;48(3):849-52.
- Clementi EM, Misiti F. Potential Health Benefits of Rhubarb. In: Watson RR, Preedy VR (Editors). Bioactive Foods in Promoting Health. 1st Edition, San Diego: Academic Press, 2010:407-423.
- Dregus M, Engel KH. Volatile constituents of uncooked rhubarb (Rheum rhabarbarum L.) stalks. J Agric Food Chem 2003;51(22):6530-6.
- Duh P, Yen G. Antioxidative activity of three water extracts. Food Chem 1997;60(4):639-45. Elmastaş M, Gülçin D, Beydemir Ş, Küfrevioğlu ÖD, Aboul-Enein HY. A study on the invitro antioxidant activity of juniper (Juniperus communis L.) seeds extracts. Anal Lett 2006;39(1):47-65.
- Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root; their relative astringency and radical scavenging effects. Chem Pharm Bull 1988;36(6):2090-7.
- Jang WH, Hsu WH, Hengel MJ, Shibamoto T. Antioxidant activity of rhubarb (Rheum rhabarbarum L.) extract and its main component emodin. Nat Prod Chem Res 2018;6(3):316-9.
- Kumar K, Ganesan K, Rao P. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty-An edible seaweed. Food Chem 2008;107(1):289-95.
- Lin CC, Wu CI, Lin TC, Sheu SJ. Determination of 19 rhubarb constituents by high-performance liquid chromatography- ultraviolet-mass spectrometry. J Sep Sci 2006;29(17):2584-93.
- Lu Y, Foo Y. Antioxidant radical scavenging activities polyphenols from apple pomace. Food Chem 2000;68(1):81-5.
- Matsuda H, Morikawa T, Toguchida I, Park JY, Harima S, Yoshikawa M. Antioxidant constituents from rhubarb: structural requirements of stilbenes for the activity and structures of two new anthraquinone glucosides. Bioorg Med Chem 2001;9(1):41-50. Menaga D, Rajkumar S, Ayyasami P. Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. Int J Pharm Pharm Sci 2013;5(4):601-6. Miliauskas G, Venskutonis PR, Van-beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85(2):231-7. Oluyemi K, Okwuonu U, Baxter D, Oyesola T. Toxic effects of methanolic extract of Aspilia africana leaf on the estrous cycle and uterine tissues of Wistar rats. Int J Morphol 2007;25(3):609-14.
- Ozgen M, Resse R, Tulio A, Scheerens J, Miller R. Modified 2,2`- azino-bis-3-ethylbezthiazoline-6 sulphonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2`-diphenyl-1-picrylhydrazyl (DPPH) methods. J Agric Food Chem 2006;54(4):1151-7.
- Öztürk M, Aydoğmuş-Öztürk F, Duru ME, Topçu G. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chem 2007;103(2):623-30.
- Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269(2):337- 41.
- Umamaheswari M, Chatterjee T. In vitro antioxidant activities of the fractions of Coccinnia grandis L. leaf extract. Afr J Trad Compl Altern Med 2008;5(1):61-73.
- Wang J, Zhao Y, Xiao X, Li H, Zhao H, Zhang P, Jin C. Assessment of the renal protection and hepatotoxicity of rhubarb extract in rats. J Ethnopharmacol 2009;124(1):18-25.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri |
| Bölüm | Orijinal Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 15 Aralık 2018 |
| Gönderilme Tarihi | 15 Ağustos 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 11 Sayı: 2 |
Van Health Sciences Journal (Van Sağlık Bilimleri Dergisi) başlıklı eser bu Creative Commons Atıf-Gayri Ticari 4.0 Uluslararası Lisansı ile lisanslanmıştır.
