Karbon tetraklorür Uygulanan Dişi Sıçanlarda Allium schoenoprasum L.’nin Serum Total Sialik Asit ve Lipid Bağlı Sialik Asit Üzerindeki Koruyucu Etkisi.

Yıl 2020, Cilt: 13 Sayı: 1, 25 - 31, 30.04.2020

Yılmaz Koçak , Gökhan Oto , Suat Ekin , Ufuk Mercan , Ahmet Bakır

Öz

Bu çalışmada, Allium schoenoprasum L. (AS) uygulamasının sıçanların serumunda total sialik asit (TSA) ve lipid bağlı sialik asit (LSA) üzerindeki etkileri karbon tetraklorür (CCL4) uygulamasını takiben incelendi. Çalışmada hayvan materyali olarak Wistar albino ırkı sıçanlar kullanıldı ve her grupta yedi sıçan olacak şekilde 10 grup oluşturuldu. A. schoenoprasum L. özü, 9 gün boyunca 100, 200, 400 mg / kg'da oral gavaj yolu ile sıçanlara uygulandı. CCL4 1 ml/kg tek doz intraperitoneal (i.p.) yolla toksisite oluşturmak için uygulandı. Çalışmanın sonunda, TSA ve LSA düzeyleri serum örneklerinde spektrofotometrik yöntem ile ölçüldü. TSA düzeyi, CCL4 grubunda en yüksek seviyeye çıkarken; CCL4 grubuna göre, kontrol ve farklı dozlarda uygulanan AS ekstresinin grupları arasında önemli fark olduğu belirlendi (p<0.01). Ancak TSA açısından CCL4 + AS ekstresi uygulanan gruplar, CCL4 grubu ile karşılaştırıldığında istatistiksel olarak anlamlı fark yoktu (p>0.05). CCL4 grubundaki serum LSA düzeyleri, kontrol ve sadece AS ektresi uygulanan gruplara göre yüksekti (p<0.001). CCL4 + AS ekstre grupları serum LSA düzeyini kontrol grubuna yaklaştırırken, CCL4 ile bu gruplar arasında istatistiksel olarak anlamlı fark bulunmadı (p>0.05). Ayrıca CCL4+Legalon grubu, kontrol ve CCL4 gruplarına göre anlamlı bir fark oluşturmadı (p>0.05). Sonuç olarak, CCL4 uygulanan sıçanların TSA ve LSA serum konsantrasyon düzeylerinin A. schoenoprasum L. uygulamasından etkilendiği bulundu. CCL4 uygulanan gruplarda, TSA ve LSA serum seviyelerindeki artışı A. schoenoprasum L. kısmen önledi. Bu çalışmanın sonuçları A. schoenoprasum L'nin serum TSA ve LSA konsantrasyon düzeyleri üzerinde olumlu bir etkisinin olduğunu göstermektedir.

Anahtar Kelimeler

Allium schoenoprasum L.., Karbon tetraklorür, Total sialik asit, Lipid bağlı sialik asit

Kaynakça

• Ağaoğlu S, Dostbil N, Alemdar S. The antibacterial efficiency of some herbs used in herby cheese. YYÜ Vet Fak Derg. 2005; 2: 39-41.
• Amalia L, Sukandar EY, Roesli RMA, Sigit JI. The effect of ethanol extract of kucai (Allium schoenoprasum L.) bulbs on serum nitric oxide level in male wistar rats. Int J of Pharmacol. 2008; 4: 487–91.
• Barazani O, Khadka UR, Golan-Goldhirsh A, Dudai N. Cadmium accumulation in Allium schoenoprasum L. grown in an aqueous medium. Chemosphere. 2004; 9: 1213-18.
• Cemek M, Aymelek F, Büyükokuroğlu ME, Karaca T, Büyükben A Yilmaz F. Protective potential of Royal Jelly against carbon tetrachloride induced-toxicity and changes in the serum sialic acid levels. 2010;10: 2827-32.
• Chai Q, Arndt JW, Dong M, Tepp WH, Johnson EA, Chapman ER, Stevens RC. Structural basis of cell surface receptor recognition by botulinum neurotoxin B. Nature. 2006; 21; 44:1096-100.
• Deliorman OD, Hartevioğlu A, Orhan N, Berkkan A, Gökbulut A, Günhan Ö, Pekcan M. Subacute Effects of Standardized Fumaria Vaillantii Lois. Ethanol extract on trace element levels. Biochemical and histopathological parameters in experimental liver toxicity. J Food Biochem. 2016; 2: 180-89.
• Dnistrian AM, Schwartz MK, Katopodis N, Frachia AA, Chester Stock. Serum Lipid-Bound Sialic Acid as a Marker in Breast Cancer, Cancer. 1982:50;1815-19. Elgawisha RAR, Rahman HGA, Abdelrazek HBMA. Green tea extract attenuates CCl4-induced hepatic injury in male hamsters via inhibition of lipid peroxidation and p53-mediated apoptosis. Toxicol. Report., 2015: 1149–56.
• El-haskoury R, Al-Waili N, Kamoun Z, Makni M, Al-Waili H, and Lyoussia B. Antioxidant activity and protective effect of Carob honey in CCl4-induced kidney and liver injury. Arch Med Res. 2018; 49: 306-13.
• Fang D, Zhang S, Dai H, Hong Z, Lin Y, Lignan and flavonoid support the prevention of cinnamon against oxidative stress related diseases. Electrochimica Acta, 2019: DOI: 10.1016/j.electacta.2019.134956.
• Haro G, Sinaga SM, Iksen I, Nerdy N, Suthinee theerachetmongkol protective effects of chives leaves (Allium Schoenoprasum L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. J App Pharm Sci 2017; 7: 222-25.
• Iksen I, Haro G, Sinaga SM. In vitro test of chive leaves infuse (Allium schoenoprasum L.) on calcium oxalate solubility using atomic absorption spectrophotometry. Int J Chem Tech Res 2017; 10: 99-102.
• Katopodis N, Hirshaut Yashar, Geller NL, Stock CC. Lipid-Associated Sialic Acid Test for the Detection of Human Cancer, Cancer Research. 1982:42; 5270-75. Khan A, Shal B, Naveed M, Shah FA, Atiq A, Khan NU. Matrine ameliorates anxiety and depression-like behaviour by targeting hyperammonemia-induced neuroinflammation and oxidative stress in CCl4 model of liver injury. Neurotoxicology. 2019; 72:38-50.
• Liang L, Shen Y, Zhang J, Xu S, Xu W, Liang C. Identification of breast cancer through spectroscopic analysis of cell-membrane sialic acid expression. Analytica Chimica Acta. 2018; 1033:148-55.
• Manautou JE, Campion SN, Aleksunes LM. Regulation of Hepatobiliary Transporters during Liver Injury. Comprehensive Toxicol, 2010: 9; 175-220.
• Mushtaq A, Naqvi SF, Anwar R, Jamil M, Anwar H, Bashir A et all. Evaluation of Hypolipidemic Activity of Allium schoenoprasum in Albino Rats, BJPR. 2016;14: 1-10. Nigam PK, Narain VS, Ajay K. Sialic acid in cardiovascular diseases. Indian Journal of Clinical Biochemistry 2006; 21 : 54-61.
• Oke GO, Abiodun AA, Imafidon CE, Monsi BF. Zingiber officinale (Roscoe) mitigates CCl4-induced liver histopathology and biochemical derangements through antioxidant, membrane-stabilizing and tissue-regenerating potentials. Toxicol Rep. 2019;6:416–25.
• Oto G, Ekin S, Özdemir H, Bulduk M, Uyar H, Öksüz E. The protective role of resveratrol on serum total sialic acid and lipid-bound sialic acid in female rats with chronic fluorosis. East J Med. 2016; 4: 168-72.
• Özben T, Elevated Serum and Urine Sialic Acid levels in Renal Diseases, Ann Clin Biochem. 1991: 28; 44-8.
• Parvu AE, Parvu M, Vlase L, Miclea P, Mot AC, Silaghi-Dumitresku R. Anti inflammatory effects of Allium schoenoprasum L. leaves. J Physiol Pharmacol 2014; 3: 309-15.
• Plucinsky MC, Riley WM, Prorok JJ, Alhadeff JA () Total and Lipid Associated Serum Sialic Acid Levels in Cancer Patients with Different Primary Sites and Differing Degrees of Metastatic Involvement, Cancer. 1986; 58: 2680-85.
• Pubchem, Carbon tetrachoride, https://pubchem.ncbi.nlm.nih.gov/compound/Carbon- tetrachloride/ 11.12.2019.
• Sengupta A, Ghosh S, Bhattacharjee S. Allium Vegetables in Cancer Prevention: An Overview: Asian Pacific J Cancer Prev. 2004; 5: 237-45.
• Singh V, Chauhan G, Krishan P, Shri R. Allium schoenoprasum L: a review of phytochemistry, pharmacology and future directions. Nat Prod Res. 2018; 32: 2202-16. Stajner D, Canadanoviç-brunet J andpavlovi A, Allium Schoenoprasum L, as a natural antioxidant. Phytother 2004; 18: 522–24.
• Stajner D, Popović BM, Ćalić-Dragosavac D, Malenčić D, Zdravković-Korać S. Comparative study on allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant status. Phytother Res. 2011; 25: 1618–22.
• Stajner D, Popović BM, Ćalić-Dragosavac D, Malenčić D, Zdravković-Korać S. Comparative study on allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant status. Phytother Res. 2011; 25: 1618–22.
• Sumitra G, Mithun R, Anand CVR. Plasma Myeloperoxidase and Total Sialic Acid as Prognostic Indicators in Acute Coronary Syndrome. JCDR, 2016; 10: 9-13.
• Suna J, Wua Y, Longa C, Hea P, Gua J, Yanga L. Anthocyanins isolated from blueberry ameliorates CCl4 induced liver fibrosis by modulation of oxidative stress, inflammation and stellate cell activation in mice. Food Chem Toxicol, 2018; 491-99.
• Surapaneni KM, Vishnu P. Serum total sialic acid, lipid peroxidation, and glutathione reductase levels in patients with rheumatoid arthritis. Turk J Med Sci. 2010; 40:537-40.
• Sydow G, A Simplified Quick Method for Determination of Sialic Acid in Serum, Biomed Biochim Acta. 1985; 44:1721-23.
• Tatlıoglu T, Allium schoenoprasum L. Genetic Improvement of Vegetable Crops. ABD: Elsevier Academic Press; 1993; 3-13.
• Tyshko NV, Shestakova SI. Model of vitamin and mineral deficiency for toxicological research:Apoptosis activity under conditions of CCL4 intoxication. Toxicol Rep. 2019; 6: 151-54.
• Varki A. Review Glycan-based interactions involving vertebrate sialic-acid-recognizing proteins.Nature. 2007;446:1023-29.
• Varki A. Sialic acids in human health and disease. Trends Mol Med. 2008;14:351-60.
• Vimr ER, Kalivoda KA, Deszo EL, Steenbergen SM. Review Diversity of microbial sialic acid metabolism. Microbiol Mol Biol Rev. 2004; 68:132-53.
• Vinoth K, Sivaraj A, Elumalai EK, Senthil K. Carbon tetrachloride-induced hepatotoxicity in rats – protective role of aqueous leaf extracts of Coccinia grandis. Int. J. Pharm. Technol. Res. 2009; 4:1612-15.
• Vinoth KP, Sivaraj A, Elumalai EK, Senthil KB. Carbon tetrachloride-induced hepatotoxicity in rats – protective role of aqueous leaf extracts of Coccinia grandis. Int. J. Pharm. Technol. Res. 2009;1:1612–15.
• Wang B, Brand-Miller J. The role and potential of sialic acid in human nutrition. Eur J Clin Nutr. 2003;57:1351-69.
• Wu Y, Wang F, Zheng Q, Lu L, Yao H, Zhou C, et all. Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage. J Biomed Sci. 2006; 13:569–78.
• Zhang C, Chen J, Liu Y, Xu D. Sialic acid metabolism as a potential therapeutic target of atherosclerosis. Lipids Health Dis. 2019; 18:173.