Echinococcus granulosus İnfeksiyonu Tanısında Moleküler Uygulamalar ve Yeni Yaklaşımlar

Yıl 2021, Cilt: 14 Sayı: 1, 114 - 121, 30.04.2021

Pervin Elvan Tokgün , Nuray Altıntaş , Onur Tokgün , Nazmiye Altıntaş

https://doi.org/10.52976/vansaglik.886786

Öz

Kistik ekinokokkozis (KE), tüm dünyaya yayılan, büyük bir hastalık yüküne neden olan ve ara konaklarda uzun süreli hidatik kist büyümesi ile karakterize, kronik zoonotik bir hastalıktır. KE etkeni olan Echinococcus granulo-sus, çoğunlukla karaciğerde (%65-70) ve akciğerlerde (%20-25) ve diğer organlarda (%2 böbrek, %2 dalak ve %2'den az beyin vb.) hidatik kistlere neden olmaktadır. KE tanısı klinik bulgulara, görüntüleme yöntemlerine, serolojik ve moleküler tekniklere dayanır. Hasta serumundaki Echinococcus DNA'sının belirlenmesi tanıda inva-ziv olmayan uygulanabilir bir yöntem olabilir. Şimdiye kadar, farklı E. granulosus genotipleri, insanlardan ve diğer ara konaklardan moleküler teknikler kullanılarak tanımlanmıştır. Ama şimdi moleküler yaklaşımlar sa-dece DNA seviyeleriyle değil, aynı zamanda RNA seviyeleriyle de sınırlıdır. Özellikle genomik, proteomik, mikrodizi ve yeni nesil dizileme analizlerindeki yeni gelişmeler, tanı, aşılama ve kemoterapi için ek hedeflerin belirlenmesinde faydalı olacaktır. Yüksek çıktılı analiz yöntemlerinin kullanılması, E. granulosus ile konakları arasındaki etkileşim mekanizmasının temelini oluşturmaya yardımcı olabilir. Böylece, elde edilen yeni bilgiler, E. granulosus enfeksiyonunun yeni tedavi ve tanısal hedeflerini geliştirmek için kullanılabilecektir

Anahtar Kelimeler

E.granulosus, kistik ekinokokkozis, moleküler teknikler, DNA, RNA

Destekleyen Kurum

Yok

Proje Numarası

Yok

Teşekkür

Yok

Echinococcus granulosus İnfeksiyonu Tanısında Moleküler Uygulamalar ve Yeni Yaklaşımlar

Öz

Cystic echinococcosis (CE) is a chronic zoonotic disease which is distributed all over the world, causes a large disease burden, and characterized by prolonged growth of hydatid cysts in intermediate hosts. Echinococcus granulosus which is a CE agent and causes hydatid cysts in mostly in liver (65-70%) and lungs (20-25%) but also other organs (kidney 2%, spleen 2% and brain less than 2%, etc.). The diagnosis of CE is based on clinical fin-dings, imaging techniques, serological and molecular technics. Identification of Echinococcus DNA in patient se-rum may be an applicable non-invasive method in the diagnosis. Up to now, different genotypes of E. granulo-sus have been identified by using molecular techniques from humans and other intermediate hosts. But now, the molecular approaches are not restricted to DNA levels but also to RNA levels. Especially new developments in genomics, proteomics, microarray, and next generation sequencing analysis will be useful for the identifica-tion of additional targets for diagnosis, vaccination, and chemotherapy Using high throughput analysis met-hodologies can help to underly the mechanism of interaction between E. granulosus and its hosts. So, obtained new informations will be used to develop new therapeutic and diagnostic targets of E. granulosus infection

Anahtar Kelimeler

E. granulosus, cystic echinococcosis, molecular technics, DNA, RNA

Proje Numarası

Yok

Kaynakça

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