Protein Eksikliği Olan Karaciğerde Medium Moleküler Peptitlerin Spektrumlarının Özellikleri

Year 2023, , 18 - 24, 19.03.2023

Fakhraddin Askerov , Gamze Çakmak , Hüseyin Karadağ

https://doi.org/10.36483/vanvetj.1192729

Abstract

Bu çalışma ile karaciğer dokusunda medium moleküler peptitlerin (SMP) üç spektrumunun belirlenmesi, eksojen ve endojen proteinler arasındaki ilişki, nükleotidler ve proteinlere verilen hasarın derecesi hakkında fikirler için bir temel oluşturabilir ve belirli yargılarda bulunmayı mümkün kılabilir. Çalışma, protein-vitamin beslenmesinde diyette % 60 kazein eksikliğinde 45 beyaz sıçan üzerinde gerçekleştirilmiştir. Deney hayvanları her grupta 15 rat olacak şekilde 3 gruba ayrılmıştır. Tüm alt gruplardaki ilk sıçan grubu (Grup I), bir vivaryumda tutulan kontrol grubu olarak belirlenmiştir. İkinci grubun (Grup II) sıçanlarına tam bir protein-vitamin diyeti verilmiştir. Grup III’e ise aynı diyet %60 protein (kazein) eksikliği ile uygulanmıştır. Grup II ve III'teki deney hayvanları 20, 30 ve 40 gün boyunca beslenmiştir. % 60 protein eksikliği olan bir protein-vitamin kompleksi ile beslenen hayvanların aylık beslenmesi üzerine yapılan çalışmaların sonuçları, 230 nm dalga boyundaki medium moleküler peptitlerin (MMP) fraksiyonlarının içeriği ile karşılaştırıldığında kontrol grubuna göre bu grupta önemli ölçüde %124 oranında artış gösterdiği belirlenmiştir. Bu çalışmaların sonuçları, protein-vitamin diyeti ve %60 protein eksikliği olan hayvanların 40 günlük beslenmesinin, 254 ve 280 nm dalga boylarında MMP içeriğinde ve 230 nm dalga boyunda MMP alt fraksiyonlarında önemli değişikliklere neden olduğunu göstermektedir. Muhtemelen hepatositlerin genetik özelliklerinden dolayı MMP alt fraksiyonlarında orta derecede bir artış söz konusudur.

Keywords

Karaciğer, Protein, Spektrum., Peptitler

Features of the Spectra of Medium-Molecular Peptides of the Liver with Protein-Deficient Nutrition

Abstract

With this study, the determination of three spectra of medium molecular peptides (SMP) in liver tissue can provide a basis for ideas about the relationship between exogenous and endogenous proteins, the degree of damage to nucleotides and proteins, and enable certain judgments to be made. The work was carried out on 45 outbred white rats against the background of full-fledged protein-vitamin nutrition and under conditions of 60% protein deficiency in the diet. The experimental animals were divided into 3 groups, 15 rats in each group. The first group (Group I) of rats in all subgroups served as controls, kept in a vivarium; rats of the 2nd group (Group II) received a complete protein-vitamin diet; Group III received the same diet, but with a 60% protein (casein) deficiency in the diet. Experimental animals of groups II and III received food for 20, 30 and 40 days. The results of studies on the background of a monthly feeding of animals with a protein-vitamin complex with a deficiency of 60% protein in the recipe showed that the content of medium molecular peptides (MMP) fractions at a wavelength of 230 nm significantly increases compared with the control group and is 124%. The results of these studies indicate that 40-day feeding of animals with a full-fledged protein-vitamin diet and 60% protein deficiency causes significant changes in the MMP content at 254 and 280nm wavelengths, and in the MMP subfractions at 230nm wavelength, there is a moderate increase in the MMP subfractions, which is possibly due to the peculiarities of the substitution of the genetic apparatus of hepatocytes.

Keywords

Liver, Protein, Spectrum., Peptides

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