Investigation of the Effects of Streptozotocin Applied in Different Doses on Rat Liver with Stereological Methods

Year 2024, , 53 - 58, 29.03.2024

Saadet Yıldız , Murat Çetin Rağbetli , Yasin Tülüce

https://doi.org/10.36483/vanvetj.1362601

Abstract

Diabetes mellitus is a group of chronic metabolic conditions, all of which are characterized by elevated blood glucose levels resulting from the body's inability to produce insulin or resistance to insulin action, or both. Insulin is the main hormone that regulates glucose production and use by body tissues and organs. Diabetes affects all systems in the body, including the liver. Streptozotocin is an antibiotic that performs pancreatic β-cell destruction and is widely used to induce experimental diabetes. In this study; It was aimed to calculate the volume and cell numbers of the morphometric changes caused by streptozotocin-induced diabetes in the rat liver using stereological methods. Twenty adult male rats were used in the study. Four groups were created randomly. Blood glucose values of all groups were measured. Diabetes was induced by administering different doses of streptozotocin (55 mg/kg and 65 mg/kg). After 48 hours, blood glucose values were measured again. Four weeks later, liver tissues of all rats from the control and experimental groups were taken under anesthesia and fixed in 10% formaldehyde. The fixed liver tissues were divided into equal parts by physical fractionation. It was fixed in paraffin after passing through dehydration stages. Histological sections were prepared. It was stained with Hematoxylin-Eosin, examined under a light microscope and photographed. The total volume of the liver was calculated using the Cavalieri Principle and the number of hepatocytes was calculated with the unbiased counting frame of the dissector method. In the diabetes-related groups, the increase in liver volume and cell number was significant compared to the control group (p<0.05). As a result, streptozotocin injection and the resulting diabetes lead to an increase in liver volume and hepatocyte numbers.

Keywords

Diabetes, Liver, Rat, Streptozotocin

Farklı Dozlarda Uygulanan Streptozotosinin Sıçan Karaciğerine Etkilerinin Stereolojik Metotlarla İncelenmesi

Abstract

Diabetes Mellitus, vücudun insülin üretememesinden veya insülin etkisine karşı dirençten veya her ikisinden kaynaklanan yüksek kan şekeri seviyeleri ile karakterize edilen bir grup kronik metabolik rahatsızlıktır. İnsülin, vücut dokuları ve organları tarafından glikoz üretimini ve kullanımını düzenleyen ana hormondur. Diyabet, karaciğer de dahil olmak üzere vücuttaki tüm sistemleri etkiler. Streptozotosin, pankreas β-hücre yıkımı gerçekleştiren bir antibiyotiktir ve deneysel diyabet oluşturmak için yaygın olarak kullanılır. Bu çalışmada, sıçan karaciğerinde sterptozotosin kaynaklı diyabetin neden olduğu morfometrik değişikliklerin hacim ve hücre sayılarının stereolojik yöntemler kullanılarak hesaplanması amaçlandı. Çalışmada erişkin 20 adet erkek sıçan kullanıldı. Rastgele dört grup oluşturuldu. Tüm grupların kan glikoz değerleri ölçüldü. Streptozotosinin farklı dozları (55 mg/kg ve 65 mg/kg) verilerek diyabet indüklendi. 48 saat sonra tekrar kan glikoz değerleri ölçüldü. Dört hafta sonra kontrol ve denek gruplarına ait tüm sıçanların anestezi altında karaciğer dokuları çıkarılarak %10’luk formaldehitte tespit edildi. Tespit edilen karaciğer dokuları fiziksel fraksiyonlama ile eşit parçalara ayrıldı. Dehidratasyon aşamalarından geçerek parafinde sabitlendi. Histolojik kesitler hazırlandı. Hematoksilen-Eozin ile boyandı ve ışık mikroskobunda incelenerek fotoğraflandı. Cavalieri Prensibi ile karaciğerin total hacmi, disektör yönteminin tarafsız sayım çerçevesi ile hepatosit sayıları hesaplandı. Diyabete bağlı gruplarda, kontrol grubuna göre karaciğer hacminde ve hücre sayısındaki artış anlamlıydı (p<0.05). Sonuç olarak, streptozotosin enjeksiyonu ve bunun sonucunda ortaya çıkan diyabet, karaciğer hacminde ve hepatosit sayılarında artışa yol açmaktadır.

Keywords

Diyabet, Karaciğer, Sıçan, Streptozotosin

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