Ratlarda Gebelikte Birinci Trimesterde Uterusta Otofajinin İmmunohistokimyasal Olarak Araştırılması

Year 2024, , 59 - 63, 29.03.2024

Dilara Uslu , Sema Uslu

https://doi.org/10.36483/vanvetj.1383504

Abstract

Plasental gelişim sırasında otofaji, özellikle trofoblast hücre proliferasyonu ve hücre ölümü gibi durumlarda moleküler düzeyde önemli bir role sahiptir. Trofoblast disfonksiyonuna bağlı anormal plasental gelişim, ciddi jinekolojik hastalıklara ve çeşitli fetal malformasyonlara neden olmaktadır. Gebeliğin 5. gününde otofajiyi araştırmak için yapılan çalışmada gebe ve gebe olmayan grupta uterus dokularında, uterus bezlerinde LC3 ve Beclin 1 (+), myometrium ve perimetriumun değerlendirilmesinde gebelikte myometrium hücrelerinde zayıf (+) gözlenirken, perimetriumda (+) reaksiyon ayırt edilememiştir. Bu çalışmada, kontrol grubu dokularına kıyasla gebeliğin ilk trimesterinde uterusta, özellikle endometrial alanlarda LC3 ve Beclin 1 yoğunluğundaki immünohistokimyasal artışın gebelik fizyolojisi, gebelikteki homeostaz ve hormonal mekanizma ile ilişkili olduğu sonucuna varılmıştır.

Keywords

Beclin1, gebelik, LC3, otofaji, rat, uterus

Project Number

Bu araştırma TUBİTAK tarafından “2209 A ”Proje dalında 1919B012205744 nolu proje olarak desteklenmiştir.

Immunohistochemical Investigation of Autophagy in the Uterus during the First Trimester of Pregnancy in Rats

Abstract

During placental development, autophagy has an important role at the molecular level, especially in cases such as trophoblast cell proliferation and cell death. Abnormal placental development due to trophoblast dysfunction causes serious gynaecological diseases and various fetal malformations. In the study conducted to investigate autophagy on the 5th day of pregnancy, in the pregnant and non pregnant group uterus tissues, uterine glands LC3 and Beclin 1 (+), in the evaluation of myometrium and perimetrium, weak (+) was observed in myometrium cells in pregnancy, while (+) reaction could not be distinguished in perimetrium. In this study, it was concluded that the immunohistochemical increase in LC3 and Beclin 1 intensity in the uterus, especially in the endometrial areas in the first trimester of pregnancy compared to the control group tissues is related to the physiology of pregnancy, homeostasis in pregnancy and hormonal mechanism.

Keywords

Autophagy, Beclin1, LC3, pregnancy, rat, uterus

Ethical Statement

Sivas Cumhuriyet Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’ndan 16.06.2022 tarihinde 65202830-050.04.04-662sayılı izin alınarak yapılmıştır.

Supporting Institution

TUBITAK

Project Number

Bu araştırma TUBİTAK tarafından “2209 A ”Proje dalında 1919B012205744 nolu proje olarak desteklenmiştir.

Thanks

Çalışmamızın gerçekleşmesinde verdikleri destekten dolayı TUBİTAK’a teşekkür ederiz.

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