Babesiosis’li Köpeklerde Kardiyak Troponin Seviyelerinin Belirlenmesi

Abstract

Köpeklerde kardiyak biyobelirteçler, kardiyak hastalıkların erken teşhisi, prognozu veya tedavisinin izlenmesinde büyük öneme sahiptir. Yüksek hassasiyetli kardiyak troponinler (hs-cTn'ler), miyokardiyal hasarın hassas göstergeleri olarak kabul edilir. Bu çalışma Babesia canis (B. canis)’le doğal enfekte köpeklerde yüksek hassasiyetli kardiyak troponin I (hs-cTnI) ve T (hs-cTnT) seviyelerinin belirlenmesi amacıyla yapılmıştır. Çalışmanın materyalini; Iğdır Üniversitesi Hayvan Hastanesine getirilen ve Kafkas Üniversitesi Parazitoloji laboratuvarı tarafından PCR analizi ile B. canis teşhisi konulan 3-4 yaş aralığındaki 15 hasta köpek çalışma grubunu oluşturdu. Kontrol grubu için aynı yaş aralığında sağlıklı 10 adet köpek seçildi. Hasta ve sağlıklı olan köpeklerden V. cephalica’dan serum tüplerine (BD Vacutainer, BD, Franklin Lakes, NJ) 5’er mL kan alınarak serumları ayrıştırıldı. Bu serumlar -20 °C muhafaza edilerek 30 gün süre içerisinde analizleri yapılarak hs-cTnI ve hs-cTnT seviyeleri belirlendi. Çalışmamızda B. canis’li köpeklerin hs-cTnI ve hs-cTnT seviyelerinin kontrol grubuna göre önemli derecede yüksek olduğu belirlendi (sırasıyla; p<0.01; p<0.05). Sonuç olarak, babesiosisli köpeklerde hs-cTnT ve hs-cTnI seviyelerinde istatistiksel olarak anlamlı bir artış olduğu tespit edilmiştir. Serum hs-cTnI ve hs-cTnT seviyelerindeki küçük değişimlerin bile prognostik açıdan önemi olduğundan B. canis’in köpeklerde miyokardiyal hasara neden olduğu söylenebilir. Ancak her geçen gün kullanımı artan troponinlerin; prognoz takibinde kullanılabilirliği, miyokardiyal hasar teşhisli hastalara tedavi uygulamanın faydalı olup olmadığını ve bu hastalarda troponin konsantrasyonlarının iyileşme ile ilişkili olup olmadığını netleştirmek için gelecekteki araştırmalara ihtiyaç vardır.

Keywords

• Babesiosis
• Kardiyak Troponin
• Köpek.

Supporting Institution

HERHANGİ BİR KURUMDAN DESTEK ALINMAMMIŞIR

Ethical Statement

ilgili belge ektedir

Determination of Cardiac Troponin Levels in Dogs with Babesiosis

Abstract

Cardiac biomarkers in dogs are of great importance in the early diagnosis, prognosis, or monitoring of treatment for cardiac diseases. High-sensitivity cardiac troponins (hs-cTns) are considered sensitive indicators of myocardial damage. This study was conducted to determine the levels of high-sensitivity cardiac troponin I (hs-cTnI) and T (hs-cTnT) in dogs naturally infected with Babesia canis (B. canis). The study material consisted of 15 sick dogs aged 3-4 years, brought to the Iğdır University Animal Hospital and diagnosed with B. canis by PCR analysis at the Kafkas University Parasitology Laboratory. For the control group, 10 healthy dogs of the same age range were selected. From both sick and healthy dogs, 5 mL of blood was taken from the V. cephalica into serum tubes (BD Vacutainer, BD, Franklin Lakes, NJ), and the serum was separated. These sera were stored at -20 °C and analyzed within 30 days to determine hs-cTnI and hs-cTnT levels. In our study, it was determined that the hs-cTnI and hs-cTnT levels of dogs with B. canis were significantly higher than those of the control group (p<0.01; p<0.05, respectively). As a result, a statistically significant increase in hs-cTnT and hs-cTnI levels was detected in dogs with babesiosis. Since even small changes in serum hs-cTnI and hs-cTnT levels are prognostically important, it can be said that B. canis causes myocardial damage in dogs. However, future research is needed to clarify the usability of troponins, which are increasingly used, in prognosis monitoring, whether it is beneficial to apply treatment to patients diagnosed with myocardial damage, and whether troponin concentrations in these patients are related to recovery.

Keywords

• Babesiosis
• Cardiac troponin
• Dog

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