Fusarium fujikuroi, Mucor racemosus: Sultan Papağanı (Nymphicus hollandicus)

Abstract

Bu çalışmada iştahsızlık, kilo kaybı ve dışkıda kötü koku şikayetleri ile getirilen Sultan papağanının (Nymphicus hollandicus Kerr, 1972) dışkı örneği mikolojik yönden incelenmiştir. Dışkı örneğinin aseptik şartlarda seri dilüsyonları yapılarak Dichloran Rose-Bengal Chloramphenicol Agar (DRBC) ve kanlı agar besiyerlerine inokülasyonu yapılmıştır. İnkübasyon sonrası örnekler saf koloniler olarak ayrılmış ve bu saf kolonilerden morfolojik, mikroskobik ve moleküler tanımlamalar yapılmıştır. Moleküler tanımlamalarda fenol-kloroform izoamilalkol DNA izolasyon yöntemi kullanılmıştır. Fungus DNA’larından PCR amplikasyonu Aydın Adnan Menderes Üniversitesi Biyoloji Bölümü Mikrobiyoloji Laboratuvarında ve hizmet alımı olarak ticari firmalarda yaptırılmıştır. Sekanslama hizmetleri Innopenta Biyoteknoloji ve Macrogen Europe tarafından yapılmıştır. ITS1 (50-TCCGTAGGTGAACCTGCGG-30) / ITS4 (50-TCCTCCGCTTATTGATATGC-30) primerleri kullanılmıştır. Fungusların tanımlanmasında nBLAST programı kullanılmış ve genetik benzerlik oranlarına göre türler eşleştirilmiştir. Sekans sonuçları GenBank sisteminde kayıt altına alınmış ve kültür koleksiyonuna eklenmiştir. Moleküler çalışmalar sonrasında Fusarium fujikuroi HBF578, Mucor racemosus HBB579 suşları tanımlanmıştır. Literatür çalışmaları incelendiğinde Fusarium fujikuroi HBF578 suşunun Sultan papağanı’ndan daha önce izole edilmediği ilk kez bu çalışmada raporlandığı görülmüştür.

Keywords

• Fusarium fujikuroi
• Mucor racemosus
• Sultan papağanı (Nymphicus hollandicus)

Fusarium fujikuroi, Mucor racemosus: Cockatiel (Nymphicus hollandicus)

Abstract

In this study, the stool sample of a cockatiel (Nymphicus hollandicus Kerr, 1972), brought with complaints of loss of appetite, weight loss and bad smell in the stool, was examined mycologically. Serial dilutions of the stool sample were made under aseptic conditions and inoculated into Dichloran Rose-Bengal Chloramphenicol Agar (DRBC) and blood agar media. After incubation, the samples were separated as pure colonies and morphological, microscopic and molecular identifications were made from these pure colonies. Phenol-chloroform isoamylalcohol DNA isolation method was used for molecular identification. PCR amplification from fungal DNAs was carried out at Aydın Adnan Menderes University Biology Department Microbiology Laboratory and by commercial companies as service procurement. Sequencing services were provided by Innopenta Biotechnology and Macrogen Europe. Primers ITS1 (50-TCCGTAGGTGAACCTGCGG-30) / ITS4 (50-TCCTCCGCTTATTGATATGC-30) were used. The nBLAST program was used to identify fungi and species were matched according to their genetic similarity rates. Sequence results were recorded in the GenBank system and added to the culture collection. After molecular studies, Fusarium fujikuroi HBF578 and Mucor racemosus HBB579 strains were identified. When the literature studies were examined, it was seen that the Fusarium fujikuroi HBF578 strain had not been isolated from the Cockatiel before and was reported for the first time in this study.

Keywords

• Cockatiel (Nymphicus hollandicus)
• Fusarium fujikuroi
• Mucor racemosus

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