Koyun Akciğer Örneklerinden Bazı Mycoplasma Türlerinin PCR ile Tespiti

Year 2026, Volume: 37 Issue: 1, 104 - 108, 29.03.2026

Muazzez Yeşilyurt , Özgül Gülaydın

https://doi.org/10.36483/vanvetj.1863229

https://izlik.org/JA43KW42XC

Abstract

Koyun yetiştiriciliği kırsal alanda verimsiz meraların değerlendirilmesi ve kaliteli hayvansal protein elde edilmesi ile önemli geçim kaynakları arasında yer almaktadır. Sürülerde karşılaşılan çeşitli enfeksiyöz hastalıklar, sürü sağlığını ve ekonomik geliri olumsuz yönde etkilemektedir. Mycoplasma kaynaklı pnömoni olguları da verim düşüklüğü, yüksek ölüm oranları, tedavi masrafları nedeniyle koyun yetiştiriciliğinde önemli sorunlara yol açmaktadır. Bu çalışmada, koyun akciğer örneklerinden bazı Mycoplasma türlerinin polimeraz zincir reaksiyonu (PCR) ile araştırılması amaçlandı. Bu doğrultuda Siirt belediye mezbahasında kesimi yapılan ve postmortem muayene sonucunda pnömonik lezyonlar gözlenen 159 koyun akciğer örneği kullanıldı. PCR analizi sonucunda incelenen örneklerin 8 (%5.03)’inde Mycoplasma spp. tespit edildi. Tespit edilen 8 örnekten sırasıyla %37.5 ve %25’inde Mycoplasma ovipneumoniae (M. ovipneumoniae) ve Mycoplasma agalactiae (M. agalactiae) identifiye edildi. Akciğer örneklerinin 1’inde ise M. arginini ve M. ovipneumoniae birlikte belirlendi. Sonuç olarak çalışmada Siirt bölgesinde koyunların solunum sistemi hastalıklarında M. ovipneumoniae, M. agalactiae ve M. arginini etkenlerinin de rol oynayabileceği belirlenmiştir. Ancak elde edilen düşük prevalans değerleri, bu etkenlerin mevcut enfeksiyonlardaki primer rolünün kısıtlı olabileceğine işaret etmektedir. Bu nedenle bölgede uygulanacak tedavi ve koruma-kontrol programlarının bu sınırlı yaygınlık verileri ışığında ve diğer olası patojenler de dikkate alınarak planlanması daha rasyonel bir yaklaşım olacaktır.

Keywords

Koyun , Mycoplasma , PCR , Pnömoni

Ethical Statement

Çalışma Siirt Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’nun 25/12/2025 tarih ve 2025/12/74 sayılı kararı ile onaylandı.

Detection of Some Mycoplasma Species in Sheep Lung Samples by PCR

https://izlik.org/JA43KW42XC

Abstract

Sheep farming is an important source of livelihood in rural areas, utilizing unproductive pastures and providing high-quality animal protein. Various infectious diseases observed in herds negatively affect herd health and economic returns. Mycoplasma-induced pneumonia cases also cause significant problems in sheep farming due to reduced productivity, high mortality rates, and treatment costs. This study aimed to detect some Mycoplasma species in sheep lung samples using polymerase chain reaction (PCR). For this purpose, lung samples were used from 159 sheep that were identified with pneumonic lesions during postmortem examination at the Siirt municipal slaughterhouse. Mycoplasma spp. was detected in 8 (5.03%) of the samples examined as a result of PCR analysis. Of the 8 samples detected as Mycoplasma spp., Mycoplasma ovipneumoniae (M. ovipneumoniae) and Mycoplasma agalactiae (M. agalactiae) were identified in 37.5% and 25%, respectively. In one of the lung samples, M. arginini and M. ovipneumoniae were identified together. As a result, the study determined that M. ovipneumoniae, M. agalactiae, and M. arginini may also play a role in respiratory system diseases in sheep in the Siirt region. However, the low prevalence values obtained indicate that the primary role of these agents in current infections may be limited. Therefore, it would be a more rational approach to plan treatment and protection-control programs to be implemented in the region in light of these limited prevalence data and taking other possible pathogens into account.

Keywords

Mycoplasma , Sheep , PCR , Pneumonia

Ethical Statement

The study was approved by the Siirt University Local Ethics Committee for Animal Experiments with decision number 2025/12/74 dated 25/12/2025.

References

• Adehan RK, Ajuwape ATP, Adetosoye AI, Alaka OO (2006). Characterization of Mycoplasmas isolated from pneumonic lungs of sheep and goats. Small Rumin Res, 63(1-2), 44-49.
• Ali BA, Abdullah MA (2024). Pathologıcal study of pnumonia in sheep and goat in abttoitr at Duhok province. Iraqi J Agric Sci, 55(4), 1367-1380.
• Awan MA, Ferhat A, Masoom YZ et al. (2012). Prevalence of Mycoplasma species by polymerase chain reaction (PCR) directly from the nasal swab samples of goats. Pak J Life Soc Sci, 10(1), 5–12.
• Bottinelli M, Schnee C, Lepri E et al. (2017). Investigation on Mycoplasma populations in pneumonic dairy lamb lungs using a DNA microarray assay. Small Rumin Res, 147, 96-100.
• Daee AA, Khodakaram-Tafti A, Derakhshandeh A, Seyedin M (2020). Identification of Mycoplasma ovipneumoniae and Mycoplasma arginini in sheep with pneumonia in North East of Iran. Iran J Vet Res, 21(1), 15-9.
• Deepika R, Vijayalakshmi S, Reddy N (2023). Molecular detection of Non-Mycoplasma mycoides cluster organisms in respiratory infections of sheep and goats. J Pharm Innov, SP-12(8), 300-304.
• Gautier-Bouchardon, AV (2018). Antimicrobial resistance in Mycoplasma spp. Microbiol Spectr, 6(4), 10-1128.
• Göçmen H (2014). Marmara Bölgesinde Koyun ve Keçilerde Mycoplasma agalactiae'nin Bakteriyolojik ve Moleküler Yöntemler ile Araştırılması. Doktora Tezi, Bursa Uludag Universitesi, Sağlık Bilimleri Enstitüsü, Bursa, Türkiye.
• Kılıc A, Kalender H, Eroksuz H, Muz A, Tasdemir B (2013). Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their moleculer typing in sheep and lamb lungs with pneumonie in Eastren Turkey. Trop Anim Health Prod, 45(7), 1525-1531.
• Lierz M, Hagen N, Harcourt-Brown N et al. (2007). Prevalence of mycoplasmas in eggs from birds of prey usingculture and a genus-specific Mycoplasma polymerase chain reaction. Avian Pathol, 36(2), 145-150.
• Lin YC, Miles RJ, Nicholas RA J, Kelly DP, Wood AP (2008). Isolation and immunological detection of Mycoplasma ovipneumoniae in sheep with atypical pneumonia, and lack of a role for Mycoplasma arginini. Res Vet Sci, 84(3), 367-373.
• Maksimović Z, Rifatbegović M, Loria GR, Nicholas RA (2022). Mycoplasma ovipneumoniae: a most variable pathogen. Pathogens, 11(12), 1477.
• McAuliffe L, Hatchell FM, Ayling RD (2003). Mycoplasma ovipneumoniae detection of. The Vet Rec, 153, 687-688.
• Mousa WS, Zaghawa AA, Elsify AM., et al. (2021). Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt. Vet world, 14(9), 2561.
• Nadhim RS, Al-Mashhadani ZI, Obead SA et al. (2025). Prevalence and control measures of mycoplasma infections in small ruminants. J Anim Health Prod, 13(s1), 136-143.
• Öztürkler O, Otlu S (2020). A phylogenetic analysis of Mycoplasma strains circulating in sheep pneumonia in the Kars region of Turkey. Turk J Vet Anim Sci, 44(4), 805-813.
• Pavone S, Crotti S, D'Avino N et al. (2023). The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in the respiratory mycoplasmosis of sheep and goats in Italy: correlation of molecular data with histopathological features. Res Vet Sci, 163, 104983.
• Sayed AE, Hafez A, Ateya , Darwish, A, Tahoun A (2025). Single nucleotide polymorphisms, gene expression and evaluation of immunological, antioxidant, and pathological parameters associated with bacterial pneumonia in Barki sheep. Irish Vet J, 78(1), 11.
• Timenetsky J, Santos LM, Buzinhani M, Mettifogo E (2006). Detection of multiple Mycoplasma infection in cell cultures by PCR. Brazilian J Med Biol Res, 39, 907-914.
• Tola S, Rizzu P, Leori G (1994). A species-specific DNA probe for the detection of Mycoplasma agalactiae. Vet microbiol, 41(4), 355-361.
• Udhayavel S, Sukumar K, Senthilkumar K, Srinivasan P, Elango A (2025). Isolation and molecular characterization of Mycoplasma ovipneumoniae associated with respiratory infection in sheep and goats in South India. Braz J Microbio, 56, 2087-2095.
• Weiser GC, Drew ML, Cassirer EF, Ward AC (2012). Detection of Mycoplasma ovipneumoniae and M. arginini in bighorn sheep using enrichment culture coupled with genus-and species-specific polymerase chain reaction. J Wildl Dis, 48(2), 449-453.