Kangal Koyununun Dil Papillalarının Işık ve Taramalı Elektron Mikroskobik Yapısı Üzerine Araştırmalar: Tat ve Mekanik Papillalar

Abstract

Bu çalışma, Orta Anadolu'da ve özellikle Sivas bölgesinde yetiştirilen Akkaraman koyun ırkının en büyük yerel tipi olan Kangal koyunlarında dil papillalarının morfolojik, histolojik ve ultrastrüktürel yapısını incelemek amacıyla yapılmıştır. Doku örnekleri gerekli doku işleme protokollerine göre hazırlandıktan sonra ışık mikroskobu (LM) ve taramalı elektron mikroskobu (SEM) ile incelenmiştir. Histolojik olarak, dilin dorsal yüzeyinin çok katlı yassı keratinize epitel ile kaplı olduğu ve altta yatan tabakaların lamina propria, submukoza, çeşitli yönlerde uzanan iyi gelişmiş iskelet kas demetleri ve seröz/seromuköz bezler içerdiği belirlenmiştir. SEM incelemeleri, papilla filiformisin bir ana gövde ve her iki yanında bulunan bir çift sekonder papilladan oluştuğunu ortaya koymuştur. Piramidal ve düzensiz şekilli olmak üzere iki tip papilla lentiformis ayırt edilmiştir. İki tip tat papillası tanımlanmıştır: papilla fungiformis ve papilla vallata. Papilla fungiformis'in tipik olarak mantar şeklinde olduğu ve yüzeylerinde tat alma gözenekleri içerdiği gözlemlenmiştir. Papilla vallata'nın ise bir hendekle çevrili olduğu ve tat alma tomurcukları içerdiği belirlenmiştir. Kangal koyununun dil papilla yapısı genel olarak diğer geviş getiren türlerle benzerlik gösterse de, papilla filiformis'te tek bir çift ikincil uzantının sürekli varlığı gibi belirli morfolojik farklılıklar tespit edilmiştir. Bu bulgular, Kangal koyunları için tanımlayıcı veriler sağlamakta olup, gelecekteki karşılaştırmalı ve fonksiyonel çalışmalar için bir temel oluşturabilir.

Keywords

• Dil
• Işık Mikroskobu
• Kangal Koyunu
• Papilla
• Taramalı Elektron Mikroskobu

Investigations on the Light and Scanning Electron Microscopic Structure of Tongue Papillae of Kangal Sheep: Taste and Mechanical

Abstract

This study was conducted to examine the morphological, histological, and ultrastructural structure of the tongue papillae in Kangal sheep, which is the largest local type of the Akkaraman sheep breed, raised in Central Anatolia and especially in the Sivas region. After the tissues were prepared according to the necessary tissue processing protocols, they were examined using light (LM) and scanning electron microscopy (SEM). Histological examination revealed that the dorsal surface of the tongue was covered with stratified squamous keratinized epithelium, and the underlying layers contained lamina propria, submucosa, well-developed skeletal muscle bundles running in various directions, and serous/seromucous glands. SEM examinations revealed that the filiform papilla consisted of a main body and a pair of secondary papillae located on either side. Two types of lentiform papilla, pyramidal and irregularly shaped, were distinguished. Two types of taste papillae were identified: fungiform papilla and circumvallate papilla. It was observed that the fungiform papillae were typically mushroom-shaped and contained taste pores on their surfaces. It was determined that the circumvallate papillae were surrounded by a trench and contained taste pores. Although the tongue papilla structure of the Kangal sheep generally shows similarities with other ruminant species, specific morphological differences were identified, such as the consistent presence of a single pair of secondary extensions in the filiform papilla. These findings provide descriptive data for Kangal sheep and may serve as a basis for future comparative and functional studies.

Keywords

• Kangal sheep
• Light Microscopy
• Papilla
• Scanning Electron Microscopy
• Tongue

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