Diz Osteoartritli Sıçanların Tedavisinde Kullanılan Borik Asitin Böbrek Dokuları Üzerindeki Etkilerinin Histopatolojik ve İmmünohistokimyasal Olarak Araştırılması

The original article was published on November 28, 2024. https://dergipark.org.tr/en/pub/vanvetj/article/1477410

Erratum Note

Şeymanur Yılmaz Taşcı adlı yazarın kurum bilgisi, yazar tarafından sehven hatalı bildirilmiştir. Düzeltilmiş kurum bilgisi: Şeymanur Yılmaz Taşcı, University of Health Sciences, Hamidiye International School of Medicine, Department of Physiology, Istanbul, Türkiye.

Abstract

Osteoartrit (OA) sıklıkla diz eklemlerinde ortaya çıkan ve kıkırdak homeostazının bozulması ile karakterize bir hastalıktır. Yarattığı sistemik enflamasyon nedeniyle sadece eklem bölgesini değil, birçok doku ve organı da etkilemektedir. Bu çalışmada, OA'ya bağlı sistemik enflamatuar reaksiyonların böbrek dokusunda oluşturduğu hasar ve borik asidin koruyucu etkisi araştırılmıştır. Çalışmada Wistar albino sıçanlar kullanıldı. Sıçanlarda monosodyum iyodoasetatın (MIA) intraartiküler enjeksiyonu ile deneysel diz OA modeli oluşturuldu. Çalışma; Kontrol, OA, OA + 4 mg Borik Asit, OA + 10 mg Borik Asit olmak üzere 4 gruptan oluşturuldu. Çalışma sonunda sıçanlardan böbrek dokuları alınarak histopatolojik ve immünohistokimyasal yöntemlerle TNF-α, IL-1β, NOS2 ve MMP13 analizleri yapıldı. Histopatolojik incelemelerde OA gruplarında tübüler epitel hücrelerinde şiddetli dejeneratif ve nekrotik değişiklikler görülmüş, bu değişiklikler borik asit uygulanan grupta doza bağlı olarak azalmıştır. İmmünohistokimyasal analizlerde ise OA uygulamasında ortaya çıkan sistemik enflamatuar reaksiyonların borik asit uygulaması ile doza bağımlı bir şekilde azaldığı tespit edildi. Sonuç olarak; OA'li sıçanlarda sistemik enflamatuar reaksiyonlar nedeniyle böbrek dokularının hasar gördüğü ve borik asidin bu hasara karşı koruyucu etkisi olduğu tespit edildi.

Keywords

• Borik asit
• Histopatoloji
• İmmünohistokimya
• Böbrek
• Osteoartrit.

Histopathological and Immunohistochemical Investigation on Effects of Boric Acid Used in Treatment of Rats with Knee Osteoartritis on Kidney Tissues

The original article was published on November 28, 2024. https://dergipark.org.tr/en/pub/vanvetj/article/1477410

Erratum Note

The institutional affiliation of the author Şeymanur Yılmaz Taşcı was inadvertently reported incorrectly by the authors. Corrected Affiliation: Şeymanur Yılmaz Taşcı, University of Health Sciences, Hamidiye International School of Medicine, Department of Physiology, Istanbul, Türkiye.

Abstract

Osteoarthritis (OA) is a disease that often occurs in the knee joints and is characterized by disruption of cartilage homeostasis. Due to the systemic inflammation it creates, it affects not only the joint area, but also many tissues and organs. In this study, the damage caused by systemic inflammatory reactions due to OA in kidney tissue and the protective effect of boric acid were investigated. Wistar albino rats were used in the study. An experimental knee OA model was created by intraarticular injection of monosodium iodoacetate (MIA) in rats. It was formed from 4 groups as Control, OA, OA + 4 mg Boric Acid, OA + 10 mg Boric Acid to work. At the end of the study, kidney tissues were taken from the rats and TNF-α, IL-1β, NOS2 and MMP13 analyzes were performed by histopathological and immunohistochemical methods. Histopathological examinations revealed severe degenerative and necrotic changes in tubular epithelial cells in the OA groups, and these changes decreased in the boric acid-administered group depending on the dose. In immunohistochemical analyzes, it was determined that systemic inflammatory reactions occurring in OA application decreased in a dose-dependent manner with boric acid application. In conclusion; It was determined that kidney tissues were damaged due to systemic inflammatory reactions in rats with OA and boric acid had a protective effect against this damage.

Keywords

• Boric acid
• Histopathology
• Immunohistochemistry
• Kidney
• Osteoarthritis

References

1. Allen KD, Thoma LM, Golightly YM (2022). Epidemiology of osteoarthritis. Osteoarthr Cartil, 30 (2), 184-195.
2. Armstrong TA, Spears JW, Lloyd KE (2001). Inflammatory response, growth, and thyroid hormone concentrations are affected by long-term boron supplementation in gilts. J Anim Sci, 79 (6), 1549-1556.
3. Bilge A, Ulusoy RG, Üstebay S, Öztürk Ö (2018). Osteoartrit. Kafkas Med Sci, 8 (1), 133-142.
4. Bodur H (2011). Türkiye’de Osteoartrite Güncel Bakış; Epidemiyoloji ve Sosyoekonomik Boyut. Turk Geriatri Derg, 7-14.
5. Bolat İ, Kapakin KAT, Kirman EM et al. (2023). Investigation of the Effects of Boric Acid Used in the Treatment of Rat Models with Knee Osteoarthritis Induced by Monosodium Iodoacetate on Liver Tissue. Harran Üniv Vet Fak Derg, 12 (2), 202-208.
6. Chien TY, Huang SKH, Lee CJ, Tsai PW, Wang CC (2016). Antinociceptive and anti-İnflammatory effects of zerumbone against mono-iodoacetate-induced arthritis. Int J Mol Sci, 17 (2), 249.
7. Dessordi R, Spirlandeli AL, Zamarioli A, Volpon JB, Navarro AM (2017). Boron supplementation improves bone health of non-obese diabetic mice. J Trace Elem Med Biol, 39, 169-175.
8. Di Francesco M, Fragassi A, Pannuzzo M et al. (2022). Management of osteoarthritis: From drug molecules to nano/micromedicines. Wiley Interdiscip Rev Nanomed Nanobiotechnol, 14 (3), e1780.

9. Dilmani, SA, Koç S, Çakır D, Gümüşderelioğlu M (2023). Organomodified nanoclay with boron compounds is improving structural and antibacterial properties of nanofibrous matrices. Eur J Pharm Biopharm, 184, 125-138.
10. Durick KA, Tomita M, Hunt C, Bradley D (2005). Evidence that boron down-regulates inflammation through the NF-KB pathway. Faseb J, 19 (5), 1705.
11. Estevez-Fregoso E, Kilic A, Rodríguez-Vera D et al. (2023). Effects of Boron-Containing Compounds on Liposoluble Hormone Functions. Inorg, 11 (2), 84.
12. Fonsi M, El Amrani A, Gervais F, Vincent P (2019). Intra-Articular Hyaluronic Acid and Chondroitin Sulfate: Pharmacokinetic Investigation in Osteoarthritic Rat Models. CTR, 9, 100573.
13. Goldring SR, Goldring MB (2004). The role of cytokines in cartilage matrix degeneration in osteoarthritis. Clin Orthop Relat Res, 427,27-36.
14. Gundogdu K, Gundogdu G, Demirkaya Miloglu F et al. (2024). Anti-inflammatory effects of boric acid in treating knee osteoarthritis: biochemical and histopathological evaluation in rat model. Biol Trace Elem Res, 202, 2744-2754.
15. Güneş A, Gezgin S, Kalınbacak K, Özcan H, Çakmak I (2017). Bor elementinin bitkiler için önemi. Bor Derg, 2 (3), 168-174.
16. Güney TG, Çalışkan A, Fatih KAR, Gündoğdu AÇ, Özbayer C (2022). Sıçan Böbrek Dokusunda Etanolün Akut Toksisitesi ve borik asitin koruyucu rolü. Biy Çeş ve Kor, 15 (1), 107-113.
17. Haveric A, Durmic-Pasic A, Alic A et al. (2020). Biochemical and histomorphological findings in Swiss Wistar rats treated with potential boron-containing therapeutic-K2 [B3O3F4OH]. J Trace Elem Med Biol, 62, 126642.
18. Ince S, Kucukkurt I, Demirel HH et al. (2014). Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats. Chemosphere, 108, 197-204.
19. Kapakin KAT, Gümüş R, Halit İ, Kapakin S, Sağlam YS (2012). Effects Of Ascorbic And Α-Lipoic Acid On Secretion Of Hsp-70 And Apoptosis İn Liver And Kidneys Of Broilers Exposed To Heat Stress. Ank Univ Vet Fak Derg, 59 (4), 279-287.
20. Kar F, Hacioglu C, Senturk C, Donmez DB, Kanbak G (2020). The role of oxidative stress, renal inflammation, and apoptosis in post ischemic reperfusion injury of kidney tissue: the protective effect of dose-dependent boric acid administration. Biol Trace Elem Res, 195 (1), 150-158.
21. Khaliq H, Juming Z, Ke-Mei P (2018). The physiological role of boron on health. Biol Trace Elem Res, 186, 31-51.
22. King AM, Glass KA, Milkowski AL, Sindelar JJ (2015). Impact of clean-label antimicrobials and nitrite derived from natural sources on the outgrowth of Clostridium perfringens during cooling of deli-style turkey breast. J Food Prot, 78 (5), 946-953.
23. Kuru R, Yarat A (2017). Bor ve sağlığımıza olan etkilerine güncel bir bakış. Clin Exp Health Sci, 7, 107-14.
24. Kuyinu EL, Narayanan G, Nair LS, Laurencin CT (2016). Animal models of osteoarthritis: classification, update, and measurement of outcomes. J Orthop Surg Res, 11 (1), 1-27.
25. Long H, Liu Q, Yin H et al. (2022). Prevalence trends of site‐specific osteoarthritis from 1990 to 2019: findings from the Global Burden of Disease Study 2019. Arthritis Rheumatol, 74 (7), 1172-1183.
26. Malfait AM (2016). Osteoarthritis year in review 2015: biology. Osteo and Car, 24 (1), 21-26.
27. Mongkhon JM, Thach M, Shi Q et al. (2014). Sorbitol-modified hyaluronic acid reduces oxidative stress, apoptosis and mediators of inflammation and catabolism in human osteoarthritic chondrocytes. J Inflamm Res, 63 (8), 691–701.
28. Ni R, Guo XE, Yan C, Wen C (2022). Hemodynamic stress shapes subchondral bone in osteoarthritis: An emerging hypothesis. J Orthop Translat, 32, 85–90.
29. Pawa S, Ali S (2006). Boron ameliorates fulminant hepatic failure by counteracting the changes associated with the oxidative stress. Chem Biol Interact, 160 (2), 89-98.
30. Perez-Rodriguez M, Garcia-Mendoza E, Farfan-Garcia ED et al. (2017). Not all boronic acids with a five-membered cycle induce tremor, neuronal damage and decreased dopamine. Neurotoxicology, 62, 92–99.
31. Selig DJ, Kress AT, Horton IM et al. (2022). safety and efficacy of intra‐articular non‐steroidal anti‐inflammatory drug injections for the treatment of osteoarthritis: A narrative review. J Clin Pharm Ther, 47 (8), 1122-1133.
32. Sogut I, Paltun SO, Tuncdemir M, Ersoz M, Hurdag C (2018). The antioxidant and antiapoptotic effect of boric acid on hepatoxicity in chronic alcohol fed rats. J Physiol Pharmacol, 96 (4), 404-411.
33. Söğüt Ö, Acar O (2020). Bor ve sağlık. Ecz Bil Derg, 9 (1), 11-7.
34. Terim KK, Imik H, Gumus R, Kapakin S, Sağlam Y (2013). Effect Of Vit E On Secretion Of Hsp-70 in Testes Of Broilers Exposed To Heat Stress. Kafkas Univ Vet Fak Derg, 19 (2).
35. Yildirim C, Cevik S, Yamaner H et al. (2022). Boric acid improves the behavioral, electrophysiological and histological parameters of cisplatin-induced peripheral neuropathy in rats. J Trace Elem Med Biol, 70, 126917.
36. Zhao K, Wen LB (2018). DMF attenuates cisplatin-induced kidney injury via activating Nrf2 signaling pathway and inhibiting NF-kB signaling pathway. Eur Rev Med Pharmacol, 22 (24), 8924–8931.