The Effect of Second Prostaglandin F2α Application with the Presynchronization Protocol on Fertility in Simmental Cows

Abstract

The objective of the study was to evaluate the effect of an additional PGF2α injection 24 hours after the first PGF2α in the timed AI Ovsynch protocol on the pregnancy/artificial insemination (P/AI) rate of Simmental cows presynchronized with PG-3-G. The study was conducted on a commercial farm with 103, lactating Simmental cows. Animals were randomly divided into two groups (1) PG-3-G Group (n=56): PGF2α on day 0; GnRH on day 3; Ovsynch initiation after on day 10. (2) PG-3-PG Group (n=47): Second PGF2αaddition 24 hours apart before timed AI to PG-3-G protocol. Parity, milk yield, and days in milk (DIM) were compared between the synchronization protocols. The pregnancy rates in the postpartum first and ≥2 TAI were similar between groups (p=0.462 and p=0.566, respectively). There were no statistical differences in the P/AI rates between groups 51.78% (29/56) and 51.06% (27/47), respectively. Multiparous cows revealed numerically higher pregnancy rates than the primiparous cows. However, P/AI results did not differ between groups (p=0.942). The mean preovulatory follicle diameter was 1.71±0.15 cm in the PG-3-G group, and 1.79±0.15 cm in the PG-3-PG group and the difference between groups was significant (p=0.047). In conclusion, administration of a second PGF2α injection to the protocol improved the P/AI numerically in multiparous cows, and preovulatory follicle size. Nevertheless, those findings need to be evaluated by conductinga larger study with a larger sample size.

Keywords

• Fertility
• GnRH
• Presynchronization
• Second PGF2α

Supporting Institution

This study did not receive any funding.

Ethical Statement

All animal experimental procedures were approved by the Experimental Animal Local Ethics Committee of Afyon Kocatepe University (approval number: 2023/49533702/94).

Thanks

The authors express their gratitude to Ballıpınar Farm for his valuable support in this study.

Simental İneklerde Presenkronizasyon Protokolünde İkinci Prostaglandin F2α Uygulamasının Fertiliteye Etkisi

Abstract

Bu çalışmanın amacı PG-3-G protokolü ile presenkronizasyon protokolü uygulanan Simental ırkı ineklerde izleyen Ovsynch protokolünde suni tohumlama öncesi uygulanan PGF2α’dan 24 saat sonra ikinci PGF2α enjeksiyonunun gebelik/suni tohumlama (G/ST) oranına etkisinin değerlendirilmesidir. Çalışma ticari bir çiftlikte yetiştirilen 103 adet sağmal Simental inekte gerçekleştirildi. Hayvanlar rastlantısal olarak iki gruba ayrıldı (1) PG-3-G Grubu(n=56): 0. gün PGF2α; 3. gün GnRH; 10. gün standart Ovsynch protokolü başlangıcı. (2) PG-3-PG Grubu(n=47): PG-3-G grubuna ilave olarak suni tohumlamadan önceki PGF2α’dan 24 saat sonra 2. PGF2α enjeksiyonu. Senkronizasyon grupları arasında parite, süt verimi ve sağılan gün sayısı (SGS) da karşılaştırıldı. Postpartum ilk ve ≥2. ST’da gruplar arası gebelik oranları bakımından fark bulunmadı (p=0.462 and p=0.566, respectively). Gruplar arasında G/ST oranları açısından istatistiksel fark tespit edilmedi, sırasıyla %51.78 (29/56) ve %51.06 (27/47). Multipar ineklerde gebelik oranları primipar ineklere göre daha yüksek olma eğilimi saptandı, ancak gruplar arasında fark önemsizdi (p=0.942). PG-3-G grubunda ortalama preovulatör folikül çapı 1.71±0.15 cm bulunurken, PG-3-PG grubunda 1.79±0.15 tespit edildi; gruplar arasındaki fark anlamlı bulundu (p=0.047). Sonuç olarak, PG-3-G protokolüne ikinci PGF2α enjeksiyonunun eklenmesinin multipar ineklerde G/ST oranının ve preovulatör folikül büyüklüğünün artırılmasına katkısı olduğu belirlendi. Bununla birlikte, çalışmada ortaya konan bulguların daha büyük örnek sayısı ile ayrıntılı olarak araştırılması gerektiği kanaatine varıldı.

Keywords

• Fertilite
• GnRH
• İkinci PGF2α
• Presenkronizasyon

Supporting Institution

Bu çalışma herhangi bir fon desteği almadı.

Ethical Statement

Tüm hayvan deney prosedürleri, Afyon Kocatepe Üniversitesi Yerel Deneysel Hayvan Etik Kurulu tarafından onaylanmıştır (onay numarası: 2023/49533702/94).

Thanks

Yazarlar, bu çalışmadaki değerli desteklerinden dolayı Ballıpınar Çiftliği'ne teşekkürlerini sunarlar.

References

1. Ambarcioglu P, Mavridis D, Yazlik MO et al. (2023). Comparison of synchronization protocols on pregnancy rate in dairy cows and heifers: A systematic review and network meta-analysis. J Hellenic Vet Med Soc, 74(2), 5657-5666.
2. Barletta RV, Carvalho PD, Santos VG et al. (2018). Effect of dose and timing of prostaglandin F2α treatments during a Resynch protocol on luteal regression and fertility to timed artificial insemination in lactating Holstein cows. J Dairy Sci, 101(2), 1730-1736.
3. Bello NM, Steibel JP, Pursley JR (2006). Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of Ovsynch in lactating dairy cows. J Dairy Sci, 89(9), 3413-3424.
4. Berean DI, Bogdan LM, Cimpean R (2025). Comparative evaluation of Ovsynch and Double Ovsynch Protocols with single and double insemination in Holstein Dairy Cows: reproductive performance and cost analysis. Animals, 15(16), 2380.
5. Bewley JM, Schutz MM (2008). An interdisciplinary review of body condition scoring for dairy cattle. The Professional Animal Scientist, 24(6), 507-529.
6. Borchardt S, Haimerl P, Pohl A, Heuwieser W (2017). Evaluation of prostaglandin F2α versus prostaglandin F2α plus gonadotropin-releasing hormone as Presynch methods preceding an Ovsynch in lactating dairy cows: A meta-analysis. J Dairy Sci, 100(5), 4065-4077.
7. Borchardt S, Pohl A, Carvalho PD, Fricke PM, Heuwieser W (2018). Effect of adding a second prostaglandin F2α injection during the Ovsynch protocol on luteal regression and fertility in lactating dairy cows: A meta-analysis. J Dairy Sci, 101(9), 8566-8571.
8. Brusveen DJ, Souza AH, Wiltbank MC (2009). Effects of additional prostaglandin F2α and estradiol-17β during Ovsynch in lactating dairy cows. J Dairy Sci, 92(4), 1412-1422.

9. Carvalho PD, Santos VG, Giordano JO et al. (2018). Development of fertility programs to achieve high 21-day pregnancy rates in high-producing dairy cows. Theriogenology, 114, 165-172.
10. Chabanenko DV, Zhelavskyi MM, Skliarov PМ et al. (2024). Postpartum complications in cows: diagnosis, treatment, prevention. Ukr J Vet Agric Sci, 7(1), 60–67.
11. Colazo MG, Helguera IL, Behrouzi A, Ambrose DJ, Mapletoft RJ (2017). Relationship between circulating progesterone at timed-AI and fertility in dairy cows subjected to GnRH-based protocols. Theriogenology, 94, 15-20.
12. Ginther OJ, Araujo RR, Palhao MP, Rodrigues BL, Beg MA (2009). Necessity of sequential pulses of prostaglandin F2alpha for complete physiologic luteolysis in cattle. Biol Reprod, 80(4), 641-648.
13. Heidari F, Dirandeh E, Pirsaraei ZA, Colazo MG (2017). Modifications of the G6G timed-AI protocol improved pregnancy per AI and reduced pregnancy loss in lactating dairy cows. Animal, 11(11), 2002-2009.
14. McCracken JA, Custer EE, Lamsa JC (1999). Luteolysis: a neuroendocrine-mediated event. Physiological Reviews, 79(2), 263-323.
15. NRC (National Research Council) (2001). Nutrient Requirements of Dairy Cattle.7th Revised Edition. National Academy Press,Washington, D.C.
16. Pursley JR, Wiltbank MC, Stevenson JS et al. (1997). Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci, 80(2), 295-300.
17. Rethmeier J, Wenzlau M, Wagner M, Wiedemann S, Bachmann L (2019). Fertility parameters in German dairy herds: associations with milk yield and herd size. Czech J Anim Sci, 64, 459-464.
18. Rezende EV, Campos CC, Moraes GF, Santos RM (2020). Factors related to uterine score and its influence on pregnancy per artificial insemination in cross bred dairy cows. Liv Sci, 241: 104231.
19. Sakaguchi M (2011). Practical aspects of the fertility of dairy cattle. J Reprod Dev, 57(1), 17-33.
20. Souza AH, Ayres H, Ferreira RM, Wiltbank MC (2008). A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology, 70(2), 208-215.
21. Stevenson JS, Pulley SL, Mellieon Jr HI (2012). Prostaglandin F2α and gonadotropin-releasing hormone administration improve progesterone status, luteal number, and proportion of ovular and anovular dairy cows with corpora lutea before a timed artificial insemination program. J Dairy Sci, 95(4), 1831-1844.
22. Stevenson JS (2016). Synchronization and artificial insemination strategies in dairy herds. Vet Clin North Am Food Anim Pract, 32, 349-364.
23. Stevenson JS, Sauls JA, Mendonça LGD, Voelz BE (2018). Dose frequency of prostaglandin F2α administration to dairy cows exposed to presynchronization and either 5-or 7-day Ovsynch program durations: Ovulatory and luteolytic risks. J Dairy Sci, 101(10), 9575-9590.
24. Tippenhauer CM, Steinmetz I, Heuwieser W et al. (2021). Effect of dose and timing of prostaglandin F2α treatments during a 7-d Ovsynch protocol on progesterone concentration at the end of the protocol and pregnancy outcomes in lactating Holstein cows. Theriogenology, 162, 49-58.
25. Young CD, Schrick FN, Pohler KG et al. (2017). A reproductive tract scoring system to manage fertility in lactating dairy cows. J Dairy Sci, 100(7), 5922-5927.
26. Wiltbank MC, Baez GM, Vasconcelos JLM (2014). The physiology and impact on fertility of the period of proestrus in lactating dairy cows. Anim Reprod, 11, 225–236.
27. Wiltbank MC, Baez GM, Cochrane F et al. (2015). Effect of a second treatment with prostaglandin F2α during the Ovsynch protocol on luteolysis and pregnancy in dairy cows. J Dairy Sci, 98(12), 8644-8654.