The Effect of Bitter Almond Oil on The Some Alterations in Liver Tissue of Experimental Diabetic Rats

Abstract

The present study was designed to evaluate the impact of bitter almond oil on malondialdehyde, reduced glutathione, total protein, fatty acid composition, A, D, E and K vitamins, cholesterol and some sterols parameters in liver tissue of experimental diabetes in rats. The rats were divided into three groups: control (C) streptozotocin (STZ), streptozotocin+bitter almond oil (STZ+BAO) groups. Diabetes induced in rats by a single intraperitoneal injection of streptozotocin (45 mg/kg). 1 ml/kg the dose bitter almond oil was intraperitoneally injected twice in a week to the streptozotocin+bitter almond oil (STZ+BAO), and additionally 2 g/500 ml dose of bitter almond seed powder was added to the drinking water of these rats. The experiment continued for 8 weeks. It was observed that MDA and total protein levels were significantly increased (p<0.001), GSH level was significantly decreased (p<0.001), palmitic, palmitoleic and arachidonic acid (p<0.05) levels were significantly decreased (p<0.001), stearic and linoleic acid levels were significantly increased (p<0.001), α-linolenic and oleic and docosahexaenoic acid levels were not changed, δ-tocopherol and vitamin D2levels were significantly decreased (p<0.001), vitamins K2, vitamin D3 (p<0.05), α-tocopherol, retinol, vitamin K1, cholesterol, stigmasterol and β-sitosterol levels were significantly increased (p<0.001) in the liver tissue of STZ group when compared to the control group. It was detected that MDA, GSH and total protein levels were significantly decreased (p<0.001), palmitoleic, linoleic, arachidonic (p<0.05) and α-linolenic acid levels were significantly decreased (p<0.001), stearic, oleic and docosahexaenoic acid levels were significantly increased (p<0.001), palmitic level was not changed, Vitamin K2, δ-tocopherol, vitamin D2, α-tocopherol, vitamin K1 (p<0.01), β-sitosterol levels were significantly increased (p<0.001), vitamin D3 level was significantly decreased (p<0.001), retinol, cholesterol and stigmasterol levels were not changed in the liver tissue of STZ+BAO group when compared to the STZ group. It was determined that the applied of bitter almonds oil was limited against the metabolic disorders of GSH, total protein, some fatty acid composition and A, D, E and K vitamins in the liver tissue of experimental diabetic rats

Keywords

• Vitamin, Cholesterol, Experimental Diabetes, Oxidative Stress, Sterols

Deneysel Diyabetin Sıçan Karaciğer Dokusunda Oluşturduğu Bazı Değişiklikler Üzerine Acı Badem Yağının Etkisi

Abstract

Bu çalışma, deneysel diyabet oluşturulan sıçanlarda acı badem yağının karaciğer dokusunda yağ asidi bileşimi, malondialdehit, indirgenmiş glutatyon, total protein, A, D, E ve K vitaminleri, kolesterol ve bazı sterol parametreleri üzerinde etkisinin araştırılması için tasarlandı. Sıçanlar kontrol (K), streptozotosin (STZ) ve streptozotosin+acı badem yağı (STZ+ABY) olmak üzere üç grubu ayrıldı. STZ gruplarına intraperitoneal enjeksiyonla streptozotosin (45 mg/kg) verilerek diyabet oluşturuldu. Acı badem yağı grubundaki sıçanlara haftada iki gün 1ml/kg dozunda intraperitoneal enjeksiyonla acı badem yağı ve ayrıca deney boyunca toz haline getirilmiş 2 gr acı badem çekirdeği, 500 ml içme suyuna eklenerek verildi. Bu uygulamalar 8 hafta boyunca sürdü. Kontrol grubuna göre, STZ grubunun karaciğer dokusunda MDA ve total protein düzeyinin anlamlı bir şekilde (p

Keywords

• Vitamin, Deneysel diyabet, Kolesterol, Oksidatif stres, Sterol

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